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[1]申乃坤,赵海,甘明哲,等.鲜甘薯原料的运动发酵单胞菌快速乙醇发酵条件[J].应用与环境生物学报,2009,15(03):405-409.[doi:10.3724/SP.J.1145.2009.00405]
 SHEN Naikun,ZHAO Hai,GAN Mingzhe,et al.Rapid Ethanol Production from Fresh Sweet Potato by Zymomonas mobilis[J].Chinese Journal of Applied & Environmental Biology,2009,15(03):405-409.[doi:10.3724/SP.J.1145.2009.00405]
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鲜甘薯原料的运动发酵单胞菌快速乙醇发酵条件()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年03期
页码:
405-409
栏目:
生物质能源专辑
出版日期:
2009-05-15

文章信息/Info

Title:
Rapid Ethanol Production from Fresh Sweet Potato by Zymomonas mobilis
作者:
申乃坤赵海甘明哲靳艳玲周玲玲戚天胜
1中国科学院成都生物研究所 成都 610041
2中国科学院研究生院 北京 100049
3成都固生科技发展有限公司 成都 610041
Author(s):
SHEN NaikunZHAO HaiGAN MingzheJIN YanlingZHOU LinglingQI Tiansheng
1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
2Graduate University of Chinese Academy of Sciences, Beijing 100049, China
3Chengdu Gusheng Sci & Tech Development Co., Ltd., Chengdu 610041, China
关键词:
鲜甘薯运动发酵单胞菌同步糖化发酵乙醇发酵
Keywords:
fresh sweet potato Zymomonas mobilis simultaneous saccharification and fermentation (SSF) ethanol fermentation
分类号:
TK6 : S531.099 : TQ920.6
DOI:
10.3724/SP.J.1145.2009.00405
文献标志码:
A
摘要:
摘 要 对运动发酵单胞菌232B同步糖化发酵(SSF)鲜甘薯快速生产燃料乙醇的条件进行了研究. 通过单因素试验和正交试验获得了乙醇发酵的最佳参数为:初始pH值6.0~7.0,硫酸铵5.0 g/kg,糖化酶量1.6 AUG/kg淀粉,初始总糖浓度200 g/kg,接种量φ=12.5%. 经过21 h发酵,乙醇浓度为95.15 g/kg,发酵效率可达94%. 同时对不灭菌发酵也进行了研究,发酵效率可达92%. 残糖的HPLC分析结果说明,发酵液中已没有葡萄糖存在,经酸水解后又出现了葡萄糖、半乳糖、甘露糖等成分,说明发酵结束后的残糖是多种低聚糖. 图4 表4 参19
Abstract:
Abstract The ethanol production with simultaneous saccharification and fermentation (SSF) by Zymomonas mobilis 232B using fresh sweet potato as raw material was studied. The effects of various factors on the yield of ethanol were investigated with the single factor and the orthogonal experiments. As a result, the optimal technical conditions were obtained from those experiments: Initial pH value 6.0~7.0, nitride 5.0 g/kg (NH4)2SO4, glucoamylase 1.6 AUG/kg starch and inoculums φ=12.5%. The ethanol yield by Z. mobilis 232B reached 95.15 g/kg, with 94% of the theoretical yield, after 21 h fermentation. The non-sterilized fermentation was also studied and its yield of ethanol reached 92% g/kg. The analysis of residual sugars by HPLC showed that there was no glucose existing in the fermentation liquor. The glucose, galactose and mannose appeared after being hydrolyzed by acid. It is indicated that the residual sugars in the final fermentation liquor were oligosaccharides. Fig 4, Tab 4, Ref 19

参考文献/References:

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备注/Memo

备注/Memo:
*国家科技支撑计划项目(No. 2007BAD78B04)和四川省科技攻关项目(No. 027. X028-037)资助 Supported by the National Key Sci & Tech Project of the 11th 5-year Plan of China (No. 2007BAD78B04) and the Provincial Key Sci & Tech Project of Sichuan, China (No. 027. X028-037)
**通讯作者 Corresponding author ( E-mail: zhaohai@cib.ac.cn)
更新日期/Last Update: 2009-07-03