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[1]陈一华,张超良,王泽立,等.玉米种子的DNA指纹计算机化鉴定[J].应用与环境生物学报,2000,6(03):223-226.
 CHEN Yihua,et al..Computerized identification of dna fingerprinting of maize seed[J].Chinese Journal of Applied & Environmental Biology,2000,6(03):223-226.
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玉米种子的DNA指纹计算机化鉴定()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
6卷
期数:
2000年03期
页码:
223-226
栏目:
综述
出版日期:
2000-06-25

文章信息/Info

Title:
Computerized identification of dna fingerprinting of maize seed
作者:
陈一华张超良王泽立贾建航孙致良金德敏王斌
(中国科学院遗传研究所北京100101) (1 山东农业大学泰安271018) (2莱阳农学院莱阳265200)
Author(s):
CHEN Yihuaet al.
(Institute of Genetics, Chinese Academy of Sciences, Beijing100101) (1 Maize Breeding Division, Shandong Agricultural University, Taian271018) (2 Laiyang Agricultural College, Laiyang265200)
关键词:
关键词 计算机应用 DNA指纹 种子鉴定 玉米 RAPD
Keywords:
Keywords computer utilization DNA fingerprinting seed identification maize RAPD
摘要:
摘要 对12个优良玉米自交系的DNA进行了RAPD分析,共筛选了250个Operon引物,其中12个引物共扩增出59个多态性产物,根据这59个多态性RAPD产物,对12个自交系进行了聚类分析,把它们分为3个群,结果与根据系谱法的聚类结果一致. 经过优选,用OPN-11扩增出的11条DNA带, 建立了这12个自交系的RAPD-DNA指纹图谱. 在该图谱中每个自交系都具有特异的DNA指纹, 可以与其它自交系相区别. 然后,用1和0分别表示各条DNA带的出现和缺失, 建立了这12个自交系的计算机化的DNA指纹, 可用于玉米自交系的真伪及纯度鉴定. 图4 表1 参6
Abstract:
Abstract 12 elite maize inbred lines were examined by means of RAPD analysis. Among the 250 Operon primers screened, 12 gave reproducible, polymorphic DNA amplification patterns. 11 bands amplified by primer OPN11 were used in the development of RAPDDNA fingerprinting of the 12 inbred lines. Each inbred line has its unique fingerprinting and can be easily distinguished from each other. The RAPD results were scored based on the presence or absence of each band and then converted to computer language with two digitals, 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of each band, respectively. The RAPDDNA fingerprinting analysis developed by this research can be used in practical seed identification of the 12 elite maize inbred lines, for both true and false test as well as purity determination. Fig 4, Tab 1, Ref 6
更新日期/Last Update: 2002-11-04