|本期目录/Table of Contents|

[1]杨海燕,施国新,徐勤松,等.Cd2+胁迫对竹叶眼子菜的毒理学效应分析[J].应用与环境生物学报,2008,14(03):366-370.
 YANG Haiyan,et al..Phytotoxicity of Cd2+ on Leaf Cells of Potamogeton malaianus[J].Chinese Journal of Applied & Environmental Biology,2008,14(03):366-370.
点击复制

Cd2+胁迫对竹叶眼子菜的毒理学效应分析()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
14卷
期数:
2008年03期
页码:
366-370
栏目:
综述
出版日期:
2008-03-25

文章信息/Info

Title:
Phytotoxicity of Cd2+ on Leaf Cells of Potamogeton malaianus
作者:
杨海燕施国新徐勤松计汪栋王红霞李阳
Author(s):
YANG Haiyan et al.
(Jiangsu Key Laboratory of Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China)
关键词:
关键词 竹叶眼子菜 Cd2+胁迫抗氧化系统超微结构
Keywords:
Keywords Potamogeton malaianus Cd2+ stress antioxidase system ultrastructure
Abstract:
Abstract The toxic effects of different gradient concentrations of Cd2+ (0, 2, 4, 6 and 8 mg L-1) on chlorophyll content, antioxidase system, MDA content, soluble sugar content, soluble protein content and ultrastructure of the cells of Potamogeton malaianus were studied. With Cd2+ increasing, the chlorophyll content, soluble protein content and the activity of superoxide dismutase (SOD) decreased drastically, while the MDA content and soluble sugar content increased. The activities of catalase (CAT) and peroxidase (POD) increased, and when reached at 2 mg L-1 they decreased then. Glutathione (GSH) and ascorbic acid (ASA) content increased, and decreased after 4 mg L-1. SDSPAGE revealed that Cd2+ reduced the amounts of four polypeptides with apparent molecular weights as 52.1×103, 42.9×103, 27.4×103 and 14.1×103, respectively, while it promoted the expression of one polypeptide with apparent molecular weight 18.1×103. Ultrastructural observation showed that the ultrastructural damage was much more serious at higher pollutant concentration. This resulted in swelling of chloroplast, disruption and disappearance of chloroplast membrane, disintegration of chloroplasts, dilatation of cristae, deformation and vacuolization of mitochondria, condensation of chromatin in nucleus, dispersion of nucleolus, and disruption of nuclear membrane and nucleoplasm flowing into cytoplasm. Generally, the response of SOD, chlorophyll and soluble protein was most sensitive to Cd2+ toxicity, which could be recommended as biomonitoring indicator of Cd2+ pollution in water body. The EC50 of Cd2+ to P. malaianus was 2.97 mg L-1 based on the experiment results. Fig 8, Ref 27
更新日期/Last Update: 2008-07-01