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[1]罗建平,曹磊,潘利华,等.稀土元素对怀槐悬浮培养细胞异黄酮合成及氧化还原态的影响[J].应用与环境生物学报,2008,14(03):362-365.
 LUO Jianping,et al..Effects of Rare Earth Elements on Isoflavone Biosynthesis and Redox Status of Maackia amurensis Cells in Suspension Cultures[J].Chinese Journal of Applied & Environmental Biology,2008,14(03):362-365.
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稀土元素对怀槐悬浮培养细胞异黄酮合成及氧化还原态的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
14卷
期数:
2008年03期
页码:
362-365
栏目:
综述
出版日期:
2008-03-25

文章信息/Info

Title:
Effects of Rare Earth Elements on Isoflavone Biosynthesis and Redox Status of Maackia amurensis Cells in Suspension Cultures
作者:
罗建平曹磊潘利华查学强
Author(s):
LUO Jianping et al.
(1School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China) (2Institute for Agricultural Application of Atomic Energy, Anhui Academy of Agricultural Sciences, Hefei 230031,China)
关键词:
关键词 稀土元素异黄酮怀槐氧化还原态
Keywords:
Keywords rare earth element cerium lanthanun isoflavone Maackia amurensis redox status
Abstract:
Abstract To interpret the effects and mechamism of rare earth elements on isoflavone biosynthesis, the changes in isoflavone concentration, phenylalanine-ammonia lyase activity, and GSH and GSSG concentrations in the suspension cultures of Maackia amurensis were studied after Ce(NO3)3 and La(NO3)3 were added into the liquid medium. The rusults showed that the optimum concentrations of Ce(NO3)3 and La(NO3)3 for isoflavone biosynthesis were 20 mmol L-1 and 10 mmol L-1, respectively. During the culture period, the change in isoflavone biosynthesis caused by the elicitation of Ce(NO3)3 or La(NO3)3 was consistent with the activity of PAL. The highest accumulation of isoflavone genistein occurred on day 4 for the elicitation of Ce(NO3)3 and day 3 for La(NO3)3, and was 271.42 μg g-1 (DW) and 279.32 μg g-1 (DW), respectively. The analysis of cellular redox status showed that the treatment of Ce(NO3)3 and La(NO3)3 changed the levels of GSH and GSSG in the cultured cells. The ratio of GSH/GSSG reached a peak value on day 3 for Ce(NO3)3 and day 2 for La(NO3)3, and it was enhanced by 0.46 and 0.47 times as compared to the control. The obtained results suggested an increase in isoflavone biosynthesis by the rare earth elements could be related to the change in cell redox status. Fig 3, Ref 16

相似文献/References:

[1]胡勤海,叶兆杰,王寿祥,等.La在模拟水生态系统中的动力学行为[J].应用与环境生物学报,1998,4(03):267.
 HU Qinhai,et al..Kinetic behaviour of La in the simulated aquatic ecosystem[J].Chinese Journal of Applied & Environmental Biology,1998,4(03):267.

更新日期/Last Update: 2008-07-01