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[1]范益军 淳泽 梅娟娟 王刚 蒲尚饶 胡亚东 赵若茜 郑世刚 罗傲雪**.镧在UV-B应力下对迭鞘石斛生理及药用成分的影响[J].应用与环境生物学报,2020,26(04):1-10.[doi:10.19675/j.cnki.1006-687x.2019.10003]
 FAN Yijun,CHUN Ze,MEI Juanjuan,et al.Effects of lanthanum on physiology and medicinal components of Dendrobium aurantiacum Rchb. f. var. denneanum (kerr) under UV-B stress[J].Chinese Journal of Applied & Environmental Biology,2020,26(04):1-10.[doi:10.19675/j.cnki.1006-687x.2019.10003]
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镧在UV-B应力下对迭鞘石斛生理及药用成分的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年04期
页码:
1-10
栏目:
研究论文
出版日期:
2020-08-25

文章信息/Info

Title:
Effects of lanthanum on physiology and medicinal components of Dendrobium aurantiacum Rchb. f. var. denneanum (kerr) under UV-B stress
作者:
范益军1 淳泽2 梅娟娟1 王刚3 蒲尚饶1 胡亚东2 赵若茜2 郑世刚2 罗傲雪1**
1 四川农业大学风景园林学院 成都 610030
2中国科学院成都生物研究所 成都 610041
3四川农业大学林学院 成都 610030
Author(s):
FAN Yijun1 CHUN Ze2 MEI Juanjuan1 WANG Gang 3 PU Shangrao1 HU Yadong2 ZHAO Ruoxi2 ZHENG Shigang2& LUO Aoxue1*
1 College of Landscape Architecture, Sichuan Agricultural University,Chengdu 610030, China
2 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
3 Forestry College, Sichuan Agricultural University,Chengdu 610030, China
关键词:
UV-B辐射抗氧化酶多糖多酚迭鞘石斛
Keywords:
lanthanum UV-B radiation antioxidant enzymes polysaccharide po lyphenols DendrobiumaurantiacumRchb. f. var. denneanum ( kerr)
DOI:
10.19675/j.cnki.1006-687x.2019.10003
摘要:
为考察镧对UV-B应力下的迭鞘石斛(DendrobiumaurantiacumRchb. f. var. denneanum(kerr))生理及药用成分的影响,本研究对UV-B辐射下的迭鞘石斛叶面喷施硝酸镧溶液,筛选出最适合迭鞘石斛生长的镧溶液浓度,并考察在此浓度作用下迭鞘石斛的生理、抗氧化酶活性及药用成分的变化。UV-B辐射按强度分为T1(1.8 mmol m -2 s -1)、T2(3.6 mmol m -2 s -1)、T3(5.4 mmol m -2 s -1),辐射时间共12天,每3天检测一次。结果表明,与其他浓度的镧溶液相比,20mg/L镧溶液能促进迭鞘石斛的光合色素含量显著增加,因此最适宜迭鞘石斛生长。当迭鞘石斛接受低剂量UV-B(1.8 mmol m -2 s -1)辐射时,处理9天时,镧元素仍然能显著促进光合作用,但当辐射强度较大(5.4 mmol m -2 s -1)时,镧元素能显著促进光合作用的时间则降低为6 d 。当迭鞘石斛接受5.4 mmol m -2 s -1的UV-B辐射12天时,同时喷施镧溶液能显著增加可溶性糖和可溶性蛋白的含量, 且显著增强过氧化氢酶(CAT)的活性,有利于缓解辐射造成的氧化伤害。当迭鞘石斛接受3.6 mmol m -2 s -1UV-B辐射3天时,喷施镧溶液能显著促进叶片中超氧化物歧化酶(SOD)活性,增强SOD清除超氧阴离子的能力。当迭鞘石斛接受低剂量(1.8 mmol m -2 s -1)UV-B辐射6 d ,同时喷施镧溶液时,会显著促进多糖和多酚的积累,提升迭鞘石斛药用价值。因此,镧元素能有效保护UV-B辐射下的迭鞘石斛,并增加迭鞘石斛的药用价值。(图10 参25)
Abstract:
Objectives: To investigate the effects of Lanthanum on the physiological and medicinal components of DendrobiumaurantiacumRchb. f. var. denneanum ( kerr) under UV-B stress. Methods: In this study, lanthanum nitrate solution was sprayed on the leaves of D.denneanum under UV-B radiation to found the most suitable concentration of lanthanum solution for the growth of D. denneanum. At the same time, the changes of some physiological indexes, antioxidant enzymes and active components of D.denneanum were tested . The experiment set three radiation intensities: low (1.8 mmol m -2 s -1), medium (3.6 mmol m -2 s -1) and high (5.4 mmol m -2 s -1). The radiation and lanthanum treatment time were 12 days, and samples were tested every three days. Results: Compared with other concentrations of lanthanum solution, 20 mg/L lanthanum solution can increase the total chlorophyll and carotenoidsn of leaves significantly increased (p < 0.05), so it is the most suitable for the growth of D. denneanum. It was found in this experiment that when D. denneanum was irradiated by the UV-B for 9 days ( the radiation was 1.8 mmol m-2 s -1), the effect of the spraying lanthanum solution group of radiation on photosynthesis was reduced, and the synthesis of photosynthetic pigment was increased. But when the radiation intensity is increased to 5.4 mmol m -2 s -1, the period that lanthanum can significantly promote photosynthesis is reduced to 6 days. When D. denneanum was irradiated by the UV-B for 12 days (the radiation was 5.4 mmol m-2 s -1), the content of soluble sugar,protein and the activity of catalase (CAT) of the spraying lanthanum group were significantly increase,which is conducive to alleviate the oxidative damage caused by radiation. While when D. denneanum w as irradiated with 3.6 mmol m-2 s -1 UV-B for 3 days, spraying lanthanum solution could significantly promote the activity of superoxide dismutase (SOD) and enhance the ability of SOD to scavenge superoxide anion. Furthermore, w hen D. denneanum w as irradiated with 1.8 mmol m-2 s -1 UV-B for 6 days, and sprayed with lanthanum solution at the same time, the polysaccharides and polyphenols production w ere significantly promoted . Conclusions: Lanthanum can effectively protect D. denneanum under UV-B radiation, and increase the medicinal value of it.

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-10-03 接受日期 Accepted: 2019-11-21
*国家自然科学基金(项目编号:31700302)、四川省科技计划国际合作项目(项目编号:2017HH0095)、四川省科技计划重点研发项目(项目编号:2017SZ0022)资助
**通讯作者 Corresponding author (E-mail: aoxueluo@sina.com)
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