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[1]晋蕾 邓晴 李才武 邓雯文 杨盛智 周宇 曾文 冯莉 张和民 何永果** 邹立扣**.幼年大熊猫断奶前后肠道微生物与血清生化及代谢物的变化*[J].应用与环境生物学报,2020,26(03):1-14.[doi:10.19675/j.cnki.1006-687x.2019.07049]
 Jin Lei,De Qing,Li Caiwu,et al.The variation of gut microbio ta, biochemical character of serum and serum metabolites before and after cub giant panda weaning*[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):1-14.[doi:10.19675/j.cnki.1006-687x.2019.07049]
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幼年大熊猫断奶前后肠道微生物与血清生化及代谢物的变化*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年03期
页码:
1-14
栏目:
研究论文
出版日期:
2020-06-25

文章信息/Info

Title:
The variation of gut microbio ta, biochemical character of serum and serum metabolites before and after cub giant panda weaning*
作者:
晋蕾1 邓晴2 李才武3 邓雯文3 杨盛智1 周宇3 曾文3 冯莉3 张和民3 何永果3** 邹立扣1**
1四川农业大学资源学院 成都 611130
2 卧龙特别行政区 卧龙 6 23004
3中国大熊猫保护研究中心 都江堰 611800
Author(s):
Jin Lei1 De Qing 2 Li Caiwu3 Deng Wenwen3 Yang Shengzhi1 Zhou Yu3 Zeng Wen3 Feng Li3 Zhang Hemin3 He Yongguo 2** & Zou Likou1**
1 College of Resources, Sichuan Agricultural University, Chengdu 611130, China
2 Wolong special administrative region, Wolong 6 23004, China
3 China Conservation and Research Center for the Giant Panda, Dujiangyan 6 1130, China
关键词:
幼年大熊猫断奶肠道菌群血液生理指标血液代谢物质
Keywords:
cub giant panda weaning gut microbiome biochemical character of serum serum metabolites
DOI:
10.19675/j.cnki.1006-687x.2019.07049
摘要:
采集3只幼年大熊猫(Ailuropoda melanoleuca)在断奶前后15天的粪便(N = 24)和血清样品(N = 24),基于高通量测序技术、生化和液相色谱-质谱检测,对其肠道菌群、血清生化指标和代谢物质的组成变化进行研究。结果发现,断奶后大熊猫肠道菌群丰富度下降,肠道内细菌由Firmicutes变为Proteobacteria为主要门。在细菌属水平,Escherichia-Shigella、Streptococcus,Clostridium、Enterococcus 和Lactobacillus的相对丰度显著下降,但Pseudomonas、Leuconostoc 和Sphingobacterium则上升(LDA > 4)。肠道真菌由Ascomycota和Basidiomycota变为Basidiomycota和Zygomycota为主要门。在真菌属水平,Microidium的相对丰度显著下降,但Cystofilobasidium、Guehomyces和Cryptococcus显著上升(P < 0.05)。此外肌酸激酶和乳酸脱氢酶断奶后显著下降,而总胆红素则上升(P < 0.05)。断奶前后发现有54个(正模式)和 46 个(负模式)显著性差异代谢物(P < 0.05),如Heme和Allantoic acid 等,这些差异物质和肌酸激酶和乳酸脱氢酶有显著相关性(P < 0.05)。本研究结果表明断奶后大熊猫肠道中高丰度的纤维素或半纤维素降解菌种类增加,为研究大熊猫肠道微生物对竹子的利用提供数据支撑;肌酸激酶、乳酸脱氢酶和总胆红素可作为大熊猫断奶时健康状况的备选标志生理指标,有助于大熊猫断奶过程中健康状态的维护。(图6 表3 参43)
Abstract:
To investigate the variation of gut microbiomta, biochemical character of serum and serum metabolites in cub giant panda before and after weaning milk were analyzed by using high-throughput sequencing technology, biochemical?detection and liquid?chromatography-tandem?mass?spectrometry. We collected 24 fecal and 24 serum samples from 3 cub giant pandas before and after weaning milk. The results showed that the richness of cub giant panda’ gut microbiome decreased after weaning . For bacterial communities, Firmicutes was the most dominant phylum before weaning, while Proteobacteria was the most dominant phylum after weaning. Escherichia-Shigella , Streptococcus, Clostridium, Enterococcus and Lactobacillus significantly decreased after weaning ( LDA > 4), while Pseudomonas , Leuconostoc and Sphingobacterium increased (LDA > 4). For fungal communities, Ascomycota and Basidiomycota were the most dominant phyla before weaning, while Basidiomycota and Zygomycota were the most dominant phyla after weaning. Microidium significantly decreased after weaning (P < 0.05), while Cystofilobasidium, Guehomyces and Cryptococcus increased significantly. Besides, CK and LDH significantly decreased after weaning, while TB significantly increased (P < 0.05). Meanwhile, 54 and 46 metabolites significantly varied during weaning both under the normal and negative model, such as heme and allantoic acid etc. Interestingly, CK and LDH had significantly association with these metabolites (P < 0.05). Therefore, our results indicated that the microbes involved in cellulose or hemicellulose digest had high er abundance after weaning and it provided?evidenceto support giant panda utilizing bamboo via gut microbiome . Meanwhile, CK, LDH and TB may be used as markers for the identification of health after weaning, which could keep the healthof cub giant panda during weaning.

备注/Memo

备注/Memo:
收稿日期 Received: 2019-07-18 接受日期 Accepted: 2019-08-30
*国家自然科学基金项目(31870246,31560070)、甘肃省高等学校科研项目(2015A-007)、甘肃省重点研发计划项目(18YF1NA051)、甘肃省高校基本科研业务费和西北师范大学青年创新团队项目
**通讯作者 Corresponding author (E-mail: fenghanq@nwnu.edu.cn)
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更新日期/Last Update: 2019-09-04