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[1]嵇少泽 勾长龙 张喜庆 王羽 田佳琪 李晨 刘继军 高云航**.病死猪堆肥高效降解复合菌系的构建及应用效果评价*[J].应用与环境生物学报,2020,26(03):1-11.[doi:10.19675/j.cnki.1006-687x.2019.07007]
 JI Shaoze,GOU Changlong,ZHANG Xiqing,et al.Construction and application of a high efficient complex microbial system to degrade dead-pig carcass in compost and assessment of its effect*[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):1-11.[doi:10.19675/j.cnki.1006-687x.2019.07007]
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病死猪堆肥高效降解复合菌系的构建及应用效果评价*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年03期
页码:
1-11
栏目:
研究论文
出版日期:
2020-06-25

文章信息/Info

Title:
Construction and application of a high efficient complex microbial system to degrade dead-pig carcass in compost and assessment of its effect*
作者:
嵇少泽1 勾长龙1 张喜庆1 王羽1 田佳琪1 李晨1 刘继军2 高云航1**
1吉林农业大学动物科技学院 长春 130118
2中国农业大学动物科技学院 北京 100083
Author(s):
JI Shaoze1 GOU Changlong1 ZHANG Xiqing1 WANG Yu1 TIAN Jiaqi1 LI Chen1 LIU Jijun2 &GAO Yunhang 1**
1College of Animal Science and Tec hnology, Jilin Agricultural University, Changchun 130118, China
2College of Animal Science and Tec hnology, China Agricultural University, Beijing 100083, China
关键词:
病死猪降解菌蛋白酶脂肪酶复合菌系
Keywords:
dead -pig degrading bacteria proteinase lipase complex microbial system
DOI:
10.19675/j.cnki.1006-687x.2019.07007
摘要:
为了缩短堆肥时间、提高堆肥产品质量,从病死猪好氧堆肥样品中筛选能高效降解蛋白质和脂肪的安全菌株,经拮抗验证后构建出病死猪堆肥高效降解复合菌系并进行现场堆肥试验。结果显示:通过分离纯化共获得36株具有尸体降解能力的安全菌株,经酶活性测定复筛出10株具有较高蛋白酶及脂肪酶活性的菌株,对其进行菌种鉴定及菌株间拮抗研究后,混合构建为5组复合菌系:A?B?C?D和E。通过比较复合菌系的蛋白酶及脂肪酶活性大小,进一步选择优选复合菌系E进行现场堆肥试验,结果表明:堆肥期间对照组和接菌各组(2%和4%)最高温度分别达到约56.3 ℃?60.8 ℃和61.2 ℃;低剂量和高剂量接菌组堆肥温度达到55.0 ℃以上的高温天数均持续约7 d,显著高于对照组的3 d(P < 0. 01);堆肥结束时,对照组和接菌各组(2%和4%)降解率分别达到78.7%?97.7%和98.0%。因此,本试验构建的复合菌系E可有效提高堆肥温度并延长高温维持时间,加速病死猪的降解。(图5 表2 参30)
Abstract:
In order to shorten the composting time and improve the quality of compost products , the strains had been screened from dead-pigs composting and a cultured system was constructed by the strains of higher lipase activity and protease activity. Then, the isolates was mixed after antagonistic verification and inoculated in dead-pig and sawdust composting with different doses. The results showed that a total of 36 bacteria with high dead-pig carcass degrading capacity and ten of them with higher lipase activity and protease activity were selected finally. The selected isolates were identified by molecular biology. After studying the antagonism among the strains, the isolates was constructed into five cultured systems as A, B, C, D and E. Cultured system E with strong protein and lipase activity was further selected for cadaveric composting experiment, t he results showed that: During composting, the highest temperatures of the control group and the inoculated group ( 2% and 4%) were 56.3 ℃, 60.8 ℃ and 61.2 ℃; The composting temperature of the 2% and 4% inoculation group could reach 55.0 ℃ and maintain at the high temperature for about 7 d, which is significantly higher than control (3 d, P < 0.01) ; At the end of composting, the dead-pig degradation rate of the inoculation groups (0%, 2% and 4%) were 78.7%, 97.7% and 98.0%. Our results indicated that sufficient amount inoculation of cultured system E ( > 2%) could effectively increase composting temperature, maintain the high temperature phase for a longer period of time and accelerate the degradation of dead-pigs by composting.

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[1]武洪杰,谭周亮,刘庆华,等.一株高浓度苯胺、苯酚降解菌的分离鉴定及降解特性[J].应用与环境生物学报,2010,16(02):252.[doi:10.3724/SP.J.1145.2010.00252]
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备注/Memo

备注/Memo:
收稿日期 Received: 2019-07-05 接受日期 Accepted: 2019-08-29
*国家重点研发计划专项(2016YFD0501400)、国家重点研发计划专项(2018YFD0501800)、国家现代农业产业技术体系建设专项(CARS-37)、吉林省教育厅项目(JJKH20180643KJ)、自治区重点研发计划项目(2017BY082)、吉林省科技发展计划项目(20190201296JC)资助
**通讯作者 Corresponding author (E-mail:gaoyunhang@163.com)
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更新日期/Last Update: 2019-09-04