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[1]于丰源 蒋礼玲 ** 贾举庆 张真真 陈思佳 牛向丽 王明雪 黄胜雄**.中华猕猴桃bZIP家族成员的比较基因组学分析*[J].应用与环境生物学报,2020,26(02):1-14.[doi:10.19675/j.cnki.1006-687x.2019.06032]
 Fengyuan YU,Liling JIANG **,et al.Comparative genomics analyses of bZIP family members in Actinidia chinensis*[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):1-14.[doi:10.19675/j.cnki.1006-687x.2019.06032]
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中华猕猴桃bZIP家族成员的比较基因组学分析*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年02期
页码:
1-14
栏目:
研究论文
出版日期:
2020-03-20

文章信息/Info

Title:
Comparative genomics analyses of bZIP family members in Actinidia chinensis*
作者:
于丰源12 蒋礼玲1 ** 贾举庆3 张真真2 陈思佳2 牛向丽2 王明雪12 黄胜雄2**
1三江源生态与高原农牧业国家重点实验室/青海大学农林科学院作物育种栽培研究所/农业农村部作物基因资源与种质创新青海科学观测试验站/国家作物种质复份库 西宁 810016
2合肥工业大学食品与生物工程学院 合肥 230009
3山西农业大学农学院 太谷 030801
Author(s):
Fengyuan YU 1 2 Liling JIANG 1 ** Juqing JIA 3 Zhenzhen ZHANG 2 Sijia CHEN 2 Xiangli NIU 2 Mingxue WANG 1 2 & Shengxiong HUANG 2 **
1 State Key Laboratory of Plateau Ecology and Agriculture / Crop Breeding & Cultivation Research Institute, Qinghai Academy of Agricultural and Forestry Sciences / Qinghai Research Station of Crop Gene Resource & Germplasm Enhancement, Ministry of Agriculture and Rural affairs of People’s Republic of China, Qinghai Univ ersity / National Duplicate Genebank for Crops, Xining 810016, China
2 School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China
3 College of Agronomy, Shanxi Agricultural University, Taigu 030801, China
关键词:
bZIP中华猕猴桃系统进化比较基因组学分析基因表达模式
Keywords:
bZIP Actinidia chinensis phylogenetic evolution comparative genomics analyses gene expression pattern
DOI:
10.19675/j.cnki.1006-687x.2019.06032
摘要:
bZIP转录因子调控着植物的生长发育和逆境胁迫应答,是植物中一类重要的转录因子。基于系统的生物信息学方法,鉴定得到79个中华猕猴桃bZIP转录因子,进化分析结果揭示中华猕猴桃bZIP家族成员在J、K、L三个分组中出现了成员的缺失。中华猕猴桃bZIP家族成员的bZIP保守域主要由碱性结构域和亮氨酸拉链结构域组成,其亮氨酸拉链结构域的第4、5肽段的第7位的亮氨酸残基存在一定程度的变异。bZIP保守域之外,在中华猕猴桃的18个bZIP家族成员中,还发现包括bZIP_C保守域在内的8种其它类型的保守域,集中分布在A、C、D和G组bZIP成员中。通过与葡萄和番茄bZIP同源基因的比较,阐明中华猕猴桃基因组的两次三倍倍增是其bZIP基因数目增加的主要原因。中华猕猴桃不同发育时期果实的转录组,揭示了参与调控果实成熟的57个bZIP基因的表达水平。本研究阐明了中华猕猴桃bZIP家族成员的进化特征、保守域组成、家族成员数目扩增的主要原因,提供了涉及果实发育的优良候选基因。(图6 表2 参28)
Abstract:
The bZIP family members belong to an important kind of transcriptors, which regulate plant growth and development, and responses to biotic and abiotic stresses in plants. Based on systematic bioinformatics methods in this study, 79 bZIP genes were identified in Actinidia chinensis. The absence of bZIP members in Group J, K and L was revealed by phylogenetic analyses. Among kiwifruit bZIP family members, the bZIP domains consist of a basic region and a leucine zipper. There were some variants in the leucine residues of 7 th position in the fourth and fifth peptide of leucine zipper region. Besides bZIP domains, another 8 kinds domains, including bZIP_C, were identified in 18 kiwifruit bZIP family members, which were distributed in Group A, C, D and G. The comparisons of bZIP homologous genes between kiwifruit, grapevine and tomato demonstrated the bZIP genes’ members expansions in kiwifruit mainly resulted from the genome triplication. The RNA-Seq data of kiwifruit fruits from different developmental stages represented the expression profiles of 57 bZIP genes, which might regulate the fruit ripening. Our studies demonstrated the evolution characteristics, domain compositions and the reasons of family members expansions of kiwifruit bZIP family members, and could provide good gene candidates for future studies of fruit ripening in A. chinensis.

相似文献/References:

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 YANG Xiaocheng,et al..Physiological effect of mechanical vibration on callus of Actinidia chinensis planch[J].Chinese Journal of Applied & Environmental Biology,2002,8(02):36.
[2]阳小成,王伯初,叶梅.不同强度的声波对猕猴桃试管苗根系发育的影响[J].应用与环境生物学报,2004,10(03):274.
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[6]陈强,刘建,陈丹阳,等.中华猕猴桃EIN3/EIL转录因子家族的成员鉴定、系统进化和基因表达模式[J].应用与环境生物学报,2018,24(02):315.[doi:10.19675/j.cnki.1006-687x.2017.05006]
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备注/Memo

备注/Memo:
收稿日期 Received: 2019-06-27 接受日期 Accepted: 2019-08-01
*国家自然科学基金青年科学基金(31760383,31440028)、青海省科技厅科技平台建设项目(2017-ZJ-Y41)和物种资源保护项目(19190170)资助
**通讯作者 (E-mail: lilingjiang1015@126.com; huangshengxiong@163.com)
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更新日期/Last Update: 2019-09-04