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[1]燕红梅 张欣钰 檀文君 陈卫民**.5种不同植物根际真菌群落结构与多样性*[J].应用与环境生物学报,2020,26(01):1-11.[doi:10.19675/j.cnki.1006-687x.2019.05010]
 YAN Hong mei,ZHANG Xin yu,et al.Biodiversity and composition of rhizosphere fungi community associated with five plant species*[J].Chinese Journal of Applied & Environmental Biology,2020,26(01):1-11.[doi:10.19675/j.cnki.1006-687x.2019.05010]
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5种不同植物根际真菌群落结构与多样性*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年01期
页码:
1-11
栏目:
研究论文
出版日期:
2020-01-25

文章信息/Info

Title:
Biodiversity and composition of rhizosphere fungi community associated with five plant species*
作者:
燕红梅12 张欣钰1 檀文君1 陈卫民12**
1陕西省农业与环境微生物重点实验室 杨凌 712100
2西北农林科技大学生命科学学院 杨凌 712100
Author(s):
YAN Hong mei1 2 ZHANG Xin yu1 TAN Wen jun1 & CHEN Wei min1 2**
1 Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, Yangling 712100, China
2 College of Life Sciences , Northwest A&F University, Yangling 7 12100, China
关键词:
根际真菌群落多样性功能
Keywords:
rhizosphere fungal community diversity function
DOI:
10.19675/j.cnki.1006-687x.2019.05010
摘要:
根际真菌在植物生长和健康方面具有非常重要的作用,然而由于分离和培养技术的限制,对根际真菌群落、多样性及功能仍缺乏认识。为明确不同植物根际真菌群落的结构与差异,揭示其潜在的生态功能,采用Illumina MiSeq技术对马蔺、葡萄、大豆、玉米等五种植物根际真菌ITS rRNA基因的ITS2区进行高通量测序,并利用FUNGuild对其功能进行预测分析。结果显示,5种植物根际中80%以上的真菌序列划分到17–45个OTUs中,优势菌门为子囊菌门,优势菌纲为座囊菌纲和粪壳菌纲等;这五种植物根际真菌群落各自聚群,其香浓指数(Shannon)和丰富度(Chao1)从大到小依次为木瓜>马蔺>葡萄>大豆>玉米;在获得的1290个OUTs中,49.76%的OTUs归属于8个功能类群,其中腐生菌约占总OTUs数的25.89%,是主要功能类群,其次是病原菌/腐生/共生过渡型、植物病原体等,葡萄、木瓜和马蔺根际中腐生菌显著高于大豆和玉米。本研究表明木瓜、马蔺和常见植物根际真菌的群落结构和多样性不同,一年生作物的根际真菌多样性小于多年生植物,进而也表明根际真菌群落及其生态功能受到植物的生活周期或类型的影响。
Abstract:
Plant rhizosphere fungi plays an important role in the plant growth promoting and health protection . However, due to the limitation of cultural technique, the difference of plant species on biodiversity and composition of rhizosphere fungal communities remains unknown. To reveal the fungal communities and functions from more plant species, we analysed the structure and composition of rhizosphere fungal communities from five plant species using Illumina sequencing of the internal transcribed spacer 2 (ITS2) region, and estimated the functions by FUNGuild. Our results showed that the most abundant fungal sequences assigned to Ascomycetes at the level of phylum, and assigned to Dothideomycetes and Sordariomycetes at the level of class . Eighty percent of the fungal sequences belonged to the 17–45 most dominant OTUs for each plant species. The Shannon diversity index and the richness (Chao1) showed the order was pawpaw > irirs > grape > soybean > maize. Principal component analysis of the relative abundances of fungal OTUs showed that samples of the same plant species typically clustered together, and fungal communities were similar among soybean, irirs and grape. Additional ly, functional assignments were made in FUNGuild, the results showed that 49.76% of total OTUs (1290 OTUs) belonged to eight trophic modes, of which saprotroph was the main group accounting for 25.89% of the total OT Us, followed by pathogens/saprophytic/symbiotic combined, plant pathogens, etc. Therefore, this study revealed that rhizosphere fungal diversity of crops is lower than perennial plants, which also showed that the rhizosphere fungal community and its potential ecological function could be affected by plant species or the different life cycle.

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-05-07 接受日期 Accepted: 2019-06-19
*国家自然科学基金面上项目(31570493)资助
**通讯作者 Corresponding author (E-mail: chenwm029@nwsuaf.edu.cn)
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更新日期/Last Update: 2019-07-05