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[1]何海燕,张丹** 徐露,高嘉宁,等.镉胁迫下双孢蘑菇发酵液中低分子量有机酸分泌量与镉含量变化*[J].应用与环境生物学报,2020,26(01):1-15.[doi:10.19675/j.cnki.1006-687x.2019.03042]
　HE Haiyan,ZHANG Dan**,et al.Changes of low molecular weight organic acids secretion and cadmium content in the fermentation broth of Agaricus bisporus under cadmium stress*[J].Chinese Journal of Applied & Environmental Biology,2020,26(01):1-15.[doi:10.19675/j.cnki.1006-687x.2019.03042]
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镉胁迫下双孢蘑菇发酵液中低分子量有机酸分泌量与镉含量变化*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年01期
页码:
1-15
栏目:
研究论文
出版日期:
2020-01-25

文章信息/Info

Title:
Changes of low molecular weight organic acids secretion and cadmium content in the fermentation broth of Agaricus bisporus under cadmium stress*
作者:
何海燕1;  2 张丹1** 徐露1;  2 高嘉宁1;  2 向宇国1;  2 王波3 吴毅1 曹龙武4
1中国科学院、水利部成都山地灾害与环境研究所 成都 610041
2中国科学院大学 北京 100049 3四川省农业科学院土壤肥料研究所 成都 610066
4四川省甘孜藏族自治州海螺沟景区管理局 泸定 626100
Author(s):
HE Haiyan1;  2;  ZHANG Dan1**;  XU Lu1;  2;  GAO Jianing1;  2;  XIANG Yuguo 1;  2 ;  WANG Bo3;  WU Yi 1& CAO LW4
1 nstitute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China
2 niversity of Chinese Academy of Sciences, Beijing 100049, China
3 oil and Fertilizer InstituteSichuan Academy of Agricultural SciencesChengdu 610066, China
4 Hailuogou Scenic Area Administration Bureau of Ganzi Tibetan Autonomous Prefecture , Luding 626100, China
关键词:
镉胁迫; 双孢蘑菇; 有机酸代谢
Keywords:
Cadmium stress;  Agaricus bisporus;  LOWOA
DOI:
10.19675/j.cnki.1006-687x.2019.03042
摘要:
为探讨镉（Cd）胁迫下对发酵液中双孢蘑菇菌丝低分子量有机酸分泌量和Cd含量的影响，以双孢蘑菇A15为材料，通过摇瓶实验，分析不同Cd胁迫时间和胁迫浓度下发酵液中甲酸、乙酸、柠檬酸、苹果酸、草酸、酒石酸、琥珀酸和富马酸等8种低分子量有机酸、Cd含量及其相关性。结果显示：随胁迫时间和浓度增加，发酵液中有机酸种类和含量均差异显著（P < 0.05）。随着胁迫时间延长，无Cd和有Cd胁迫下，发酵液中甲酸、苹果酸、琥珀酸和乙酸含量变化趋势一致，甲酸基本呈增加的趋势，苹果酸和琥珀酸呈先增后减趋势，乙酸呈“双峰”模式；草酸、酒石酸、富马酸和柠檬酸含量变化趋势存在差异，无Cd胁迫下，草酸与酒石酸基本呈增加趋势，柠檬酸和富马酸呈减少趋势，Cd胁迫下，草酸和酒石酸呈“双峰”模式，柠檬酸和富马酸呈先增后减的趋势。双孢蘑菇Cd富集量随胁迫浓度的增大而增加，随胁迫时间延长先增后减。相关分析表明：胁迫前期（2 h-1 d），发酵液中Cd含量与富马酸、柠檬酸、苹果酸和乙酸呈显著负相关，与琥珀酸呈显著正相关；胁迫后期（3 d-7 d），与草酸呈显著负相关，与苹果酸呈显著正相关；整个胁迫过程中，与酒石酸呈显著正相关。低Cd胁迫下（0.50和1.00 mg/L），发酵液中Cd含量与草酸、酒石酸、苹果酸、乙酸、柠檬酸、富马酸和琥珀酸呈显著负相关；高Cd胁迫下（2.00、6.00和15.00 mg/L），与草酸、酒石酸、甲酸、乙酸、琥珀酸和总酸含量呈显著正相关，与柠檬酸和富马酸呈显著负相关。Cd胁迫能够显著地影响双孢蘑菇菌丝分泌的有机酸的种类和含量，有机酸在双孢蘑菇在抵抗Cd起着重要作用。（图4 表4 参50）
Abstract:
In order to find the effects of cadmium (Cd) stress on the secretion of low molecular weight organic acids (LMWOA) by Agaricus bisporus mycelia and the contents of Cd in the fermentation broth. The contents of Cd and 8 kinds of LMWOA, including formic acid, acetic acid, citric acid, malic acid, oxalic acid, tartaric acid, succinic acid and fumaric acid , in fermentation broth and their correlation under different Cd stress time and Cd stress concentration were analyzed by shaking flask experiment using Agaricus bisporus A15 as material. The results found: The types and contents of LMWOA in fermentation broth were significantly different with the increase of stress time and concentration (P < 0.05 ). With the prolongation of stress time, the change trends of formic acid, malic acid, succinic acid and acetic acid in the fermentation broth were the consistent. Formic acid increased basically, malic acid and succinic acid increased first and then decreased, acetic acid showed a "double peak" pattern. The change trends of oxalic acid, tartaric acid, fumaric acid and citric acid were different . Without Cd stress, oxalic acid and tartaric acid increased, citric acid and fumaric acid decreased. Under Cd stress, oxalic acid and tartaric acid showed a "double peak" pattern, while citric acid and fumaric acid increased first and then decreased. Cd concentration of Agaricus bisporus increased with the increase of Cd concentration, and increased first and then decreased with the extension of Cd stress time. The correlation analysis showed that: At 2h, 8h and 1d of Cd stress, the content of Cd in fermentation broth was negatively correlated with fumaric acid, citric acid, malic acid and acetic acid, positively correlated with succinic acid. At 3d, 5d and 7d of Cd stress, the content of Cd was negatively correlated with oxalic acid and positively correlated with malic acid. During the whole process, the content of Cd was positively correlated with tartaric acid. Under 0.50 and 1.00 mg/ Cd stress, the content of Cd was negatively correlated with oxalic acid, tartaric acid, malic acid, acetic acid, citric acid, fumaric acid and succinic acid . Under 2.00、6.00 and 15.00 mg/L Cd stress , the content of Cd in culture medium was positively correlated with oxalic acid, tartaric acid, formic acid, acetic acid, succinic acid and total acid, negatively correlated with citric acid and fumaric acid. Obviously, Cd stress could significantly affect the kinds and contents of LOWOA secreted by Agaricus bisporus mycelia , which play an important role in Cd resistance of Agaricus bisporus.

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-03-20 接受日期 Accepted: 2019-05-29
*国家自然科学基金项目（4157012180）资助
**通讯作者 Corresponding author (E-mail: daniezhang@imde.ac.cn)
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更新日期/Last Update: 2019-06-19