|本期目录/Table of Contents|

[1]张玥,胡雲飞,王树茂,等.茶园年限对根际土壤真菌群落结构及多样性的影响[J].应用与环境生物学报,2018,24(05):972-977.[doi:10.19675/j.cnki.1006-687x.2018.04011]
　ZHANG Yue,HU Yunfei,WANG Shumao,et al.The structure and diversity of the fungal community in rhizosphere soil from tea gardens of different ages[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):972-977.[doi:10.19675/j.cnki.1006-687x.2018.04011]
点击复制

茶园年限对根际土壤真菌群落结构及多样性的影响
分享到：

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年05期
页码:
972-977
栏目:
土壤微生物资源与生态专栏
出版日期:
2018-10-25

文章信息/Info

Title:
The structure and diversity of the fungal community in rhizosphere soil from tea gardens of different ages
作者:
张玥; 胡雲飞; 王树茂; 柯子星; 高水练; 林金科
福建农林大学安溪茶学院 福州 350002
Author(s):
ZHANG Yue;  HU Yunfei;  WANG Shumao;  KO Tzuhsing;  GAO Shuilian & LIN Jinke**
Anxi College of Tea Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
茶树; 根际土壤; 真菌群落; 高通量测序; 多样性
Keywords:
Camellia sinensis L.;  rhizosphere soil;  fungal community;  high-throughput sequencing;  Alpha diversity
分类号:
S571.1
DOI:
10.19675/j.cnki.1006-687x.2018.04011
摘要:
为探明不同年限茶园根际土壤真菌菌群结构及多样性的差别，采用MiSeq高通量测序方法分析5年、10年、25年和40年茶园根际土壤真菌的菌落组成、Alpha多样性、Beta多样性和组间差异性. 结果显示，随着茶园年限增大，根际土壤pH值显著性降低；而土壤养分含量呈现先增后减趋势，其中25年茶园最高. 不同年限茶园根际土壤真菌群落α多样性无显著差异，β多样性则5年与10年茶园相近，而25年和40年茶园相似. 5年和40年茶园土壤中的优势菌目存在明显差异，其中粪壳菌目占5年茶园总菌群的20.93%，糙孢孔目占40年茶园总菌群的14.97%. 此外，不同年限茶园根际土壤中差异性显著的菌种主要分布在粪壳菌纲和银耳菌纲. 本研究表明，不同年限茶园根际土壤真菌物种组成丰富、结构差异明显；随着茶园年限的增加，茶树根际土壤肥力增加和真菌群落数量增加，有利于茶树生长代谢的真菌种群增多. （图4 表2 参36）
Abstract:
To explore differences in fungal community structure and diversity in the rhizosphere soil of tea plants in tea gardens among different ages, the composition, alpha diversity, beta diversity, and group variability of fungal communities in the rhizosphere soil of 5-, 10-, 25-, and 40-year-old tea gardens (Y5, Y20, Y25, Y20) were studied using the Miseq platform. Results showed that the pH of the rhizosphere soil decreased as the age of the tea garden increased, while the soil nutrient content increased with age, except that this began to decrease in the 40-year-old tea garden. No significant difference in the fungal community’s alpha diversity was found in the rhizosphere soil among different tea garden ages. The structure and beta diversity of the fungal community in the rhizosphere soil of the 5- and 10-year-old tea gardens were similar to each other, as were those of the 25- and 40-year-old tea gardens. Certain dominant fungal species were most notably abundant in the rhizosphere soil of 5- and 40-year-old tea gardens. The order Sordariales accounted for 20.93% of fungi in the 5-year-old tea garden, and species of Trechisporales accounted for 14.97% of those in the 40-year-old tea garden. In addition, the rhizosphere soil of the tea gardens of different ages mainly contained fungi distributed among the Sordariales and Tremellomycetes orders. Overall, the fungal community in the rhizosphere soil of tea gardens of different ages was abundant overall and showed obvious differences in community structure among ages. The rhizosphere soil fertility and the amount of species in the fungal community increased with the age of the tea garden, favoring the increases in the populations of fungi that facilitate the growth and metabolism of tea plants.

参考文献/References:

1. Li SY, Wu X, Xue H, Gu BJ, Cheng H, Zeng JM, Peng CH, Ge Y, Chang J. Quantifying carbon storage for tea plantations in China [J]. Agric Ecos Environ, 2011, 141: 390-398
2. 伍丽, 余有本, 周天山, 李冬花, 冯雪, 蒋堃. 茶树根际土壤因子对根际微生物数量的影响[J]. 西北农业学报, 2011, 20 (4): 159-163 [Wu L, Yu YB, Zhou TS, LI DH, Feng X, Jiang K. Effects of soil factors on the distributions of tea rhizosphere microorganisms [J]. Acta Agric Bor-occids Sin, 2011, 20 (4): 159-163]
3. Mohammad N, Samar M, Alireza I. Levels of Cu, Zn, Pb and Cd in the leaves of the tea plant (Camellia sinensis) and in the soil of Gilan and Mazandaran farms of Iran [J]. Food Meas, 2014, 8: 277-282
4. Singh S, Pandey A, Chaurasia B, Palni LMS. Diversity of arbuscular mycorrhizal fungi associated with rhizosphere of tea growing in ‘natural’ and ‘cultivated’ ecosites [J]. Biol Fertil Soil, 2008, 44: 491-500
5. 余继忠, 徐家明, 黄海涛, 杨亚军. 重修剪、台刈和改植换种三种茶园改造方式的比较[J]. 茶叶科学, 2008, 28 (3): 221-227 [Yu JZ, Xu JM, Huang HT, Yang YJ. Comparison on the different rehabilitation methods of heavy pruning, collar pruning and replanting [J]. J Tea Sci, 2008, 28 (3): 221-227]
6. Han WY, Kemmitt SJ, Brookes PC. Soil microbial biomass and activity in chinese tea gardens of varying stand age and productivity [J]. Soil Biol Biochem, 2007, 39 (7): 1468-1478
7. 李玮, 郑子成, 李廷轩. 不同植茶年限土壤团聚体碳氮磷生态化学计量学特征[J]. 应用生态学报, 2015, 26 (1): 9-16 [Li W, Zheng ZC, Li TX, Liu MY. Ecolgical stoichiometry of soil carbon, nitrogen and phosphorus within soil aggregates in tea plantations with different ages [J]. Chin J Appl Ecol, 2015, 26 (1): 9-16]
8. Yao H, He Z, Wilson MJ, Campbell CD. Microbial biomass and community structure in a sequence of soils with increasing fertility and changing land use [J]. Microb Ecol, 2000, 40 ( 3): 223-237
9. 邢会琴, 肖占文, 闫吉智, 马建仓, 孟嫣. 玉米连作对土壤微生物和土壤主要养分的影响[J]. 草业科学, 2011, 28 (10): 1777-1780 [Xing HQ, Xiao ZW, Yan JZ, Ma JC, Meng Y. Effects of continuous cropping of maize on soil microbes and main soil nutrient [J]. Prata Sci, 2011, 28 (10): 1777-1780]
10. David C, Mark D, John H. Soil respiration from four aggrading forested watersheds measured over a quarter century [J]. For Ecol Manage, 2002, 157: 247-253
11. 王海斌, 叶江华, 陈晓婷, 贾小丽, 孔祥海. 连作茶树根际土壤酸度对土壤微生物的影响[J]. 应用与环境生物学报, 2016, 22 (3): 480-485 [Wang HB, Ye JH, Chen XT, Jia XL, Kong XH. Effect on soil microbes of the rhizospheric soil acidity of tea tree continuous cropping [J]. Chin J Appl Environ Biol, 2016, 22 (3): 480-485]
12. 贾辉, 陈秀蓉, 芦光新, 孔雅丽, 杨成德. 纤维素降解细菌的筛选、生物学特性及降解效果[J]. 草业学报, 2016, 25 (3): 60-66 [Jia H, Chen XR, Lu GX, Kong YL, Yang CD. Isolation of cellulose-degrading bacteria and determination of their degradation activity [J]. Acta Prata Sin, 2016, 25 (3): 60-66]
13. Marschner P, Crowley D, Yang CH. Development of specific rhizosphere bacterial communities in relation to plant species, nutrition and soil type [J]. Plant Soil, 2004, 261: 199-208
14. 胡雲飞, 张玥, 张彩丽, 杨路成, 陈暄, 庄静, 黎星辉. 茶树根系内生真菌与根际土壤真菌的季节多样性分析[J]. 南京农业大学学报, 2013, 36 (3): 41-46 [Hu YF, Zhang Y, Zhang CL, Yang LC, Chen X, Zhuang J, Li XH. Diversity analysis of the endophytic fungi in root and fungi in rhizosphere soil of tea plant in different seasons [J]. J Nanjing Agric Univ, 2013, 36 (3): 41-46]
15. 吴丽莎, 王玉, 赵青华, 李敏, 刘润进. 丛枝菌根真菌对实生茶树叶片光合性能的影响[J]. 青岛农业大学学报(自然科学版), 2011, 28: 13-15 [Wu LS, Wang Y, Zhao QH, Li M, Liu RJ. Effects of arbuscular mycorrhizal fungi on photosynthetic characteristics of tea seedlings [J]. J Qingdao Agric Univ (Nat Sci Ed), 2011, 28: 13-15]
16. 成泽艳. 茶树根际微生物区系分析及生物菌肥的初步研制[D]. 雅安: 四川农业大学, 2004 [Cheng ZY. Study on rhizosphere microflora and biofertilizer of tea [D]. Ya’an: Sichuan Agricultural University, 2004]
17. 谢光新, 张荣先, 黄雪飞, 王先华. 不同生长年限茶树根际微生物分布的差异[J]. 湖北农业科学, 2012, 51: 3177-3179 [Xie GX, Zhang RX, Huang XF, Wang XH. Distribution of root and rhizosphere microorganism in tea tree at dfferent ages [J]. Hubei Agric Sci, 2012, 51: 3177-3179]
18. 杨扬, 刘炳君, 房江育, 胡长玉, 程东华, 王世强. 不同植茶年龄茶树根际与非根际土壤微生物及酶活性特征研究[J]. 中国农学通报, 2011, 27 (27): 118-121 [Yang Y, Liu BJ, Fang JY, Hu CY, Cheng DH, Wang SQ. The study on characteristics of microbes and enzyme activity in rhizosphere and out-rhizosphere soil of tea garden at various ages [J]. Chin Agric Sci Bull, 2011, 27 (27): 118-121]
19. Wang Y, Guo LD. A comparative study of endophytic fungi in needles, bark, and xylem of Pinus tabulaeformis [J]. Can J Bot, 2007, 85 (10): 911-917
20. Gilbert JA, Field D, Huang Y, Edwards R, Li WZ, Gilna P, Joint I. Detection of large numbers of novel sequences in the metatranscriptomes of complex marine microbial communities [J]. PLoS ONE, 2008, 3 (8): e3042
21. Teixeira LCRS, Peixoto RS, Cury JC, Sul WJ, Pellizari VH, Tiedje J, Rosado AS. Bacterial diversity in rhizosphere soil from Antarctic vascular plants of Admiralty Bay, maritime Antarctica [J]. ISME J, 2010, 4 (8): 989-1001
22. 鲍士旦. 土壤农化学分析[M]. 3版. 北京: 中国农业出版社, 2005 [Bao SD. Soil and Agricultural Chemistry Analysis [M]. 3rd ed. Beijing: China agriculture press, 2005]
23. Gobat JM, Aragno M, Matthey W. The Living Soil: Fundamentals of Soil Science and Soil Biology [M]. New York: Science Publishers, 2004: 622
24. Banning NC, Gleeson DB, Grigg AH, Grant CD, Andersen GL, Brodie EL, Murphy DV. Soil microbial community successional patterns during forest ecosystem restoration [J]. Appl Environ Microbiol, 2011, 77 (17) : 6158-6164
25. Harris J. Soil microbial communities and restoration ecology: facilitators or followers? [J]. Science, 2009, 325 (5940): 573-574
26. Xue D, Yao H, Huang C. Microbial biomass, N mineralization and nitrification, enzyme activities, and microbial community diversity in tea orchard soils [J]. Plant Soil, 2006, 288: 319-331
27. Shanthiyaa V, Saravanakumar D, Rajendran L, Karthikeyan G, Prabakar K, Raguchander T. Use of chaetomium globosum for biocontrol of potato late blight disease [J]. Crop Prot, 2013, 25: 33-38
28. Daynes CM, Mcgee PA, Midgley DJ. Utilisation of plant cell-wall polysaccharides and organic phosphorus substrates by isolates of Aspergillus and Penicillium isolated from soil [J]. Fung Ecol, 2008, 1 (2-3): 94-98
29. Zhu XJ, Hu YF, Chen X, Wang YH, Fang WP, Li XH. Endophytic fungi from camellia sinensis show an antimicrobial activity against the rice blast pathogen Magnaporthe grisea [J]. Phyt-Int J Exp Bot, 2014, 83: 57-63
30. 葛德永, 姚槐应, 黄昌勇. 茶园土壤耐酸铝微生物的分离鉴定及其耐铝特性研究[J]. 浙江大学学报: 农业与生命科学版, 2007, 33 (6): 626-632 [Ge DY, Yao HY, Huang CY. Isolation and characterization of acid- and Al-tolerant microorganisms [J]. J Zhejiang Univ (Agric Life Sci), 2007, 33 (6) : 626-632]
31. Yang HX, Guo SX, Liu RJ. Characteristics of arbuscular mycorrhizal fungal diversity and functions in saline-alkali land [J]. Chin J Appl Ecol, 2015, 26 (1): 311-320
32. Shu B, Li WC, Liu LQ, Wei YZ, Shi SY. Transcriptomes of arbuscular mycorrhizal fungi and litchi host interaction after tree girdling [J]. Front Microbiol, 2016, 7: 408
33. Singh S, Pandey A, Kumar B, Palni LMS. Enhancement in growth and quality parameters of tea [Camellia sinensis (L.) O. Kuntze] through inoculation with arbuscular mycorrhizal fungi in an acid soil [J]. Biol Fertil Soils, 2010, 46: 427-433
34. 许平辉, 王飞权, 齐玉岗, 张墦, 杨乔, 肖斌. 丛枝菌根真菌对茶树抗旱性的影响[J]. 西北农业学报, 2017, 26 (7): 1033-1040 [Xu PH, Wang FQ, Qi YG, Zhang F, Yang Q, Xiao B. Effect of arbuscular mycorrhiza fungi on drought resistance in tea plant (Camellia sinensis) [J]. Acta Agric Bor-occids Sin, 2017, 26 (7): 1033-1040]
35. Barendsen RL, Pieterse CM, Bakker PA. The rhizosphere microbiome and plant health [J]. Trends Plant Sci, 2012, 17 (8) : 478-486
36. Pandey A, Palni LMS. The rhizosphere effect of tea on soil microbes in a Himalayan monsoonal location [J]. Biol Fertil Soils, 1996, 21: 131-137
37.

相似文献/References:

[1]刘慧芬,王卫卫,曹桂林,等.氢气对刺槐根际土壤微生物种群和土壤酶活性的影响[J].应用与环境生物学报,2010,16(04):515.[doi:10.3724/SP.J.1145.2010.00515]
　LIU Huifen,WANG Weiwei,CAO Guilin,et al.Effect of Hydrogen on Microbial Population and Enzyme Activity in Robinia pseudoacacia Rhizosphere Soil[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):515.[doi:10.3724/SP.J.1145.2010.00515]
[2]贾夏,董岁明,周春娟.低含量Pb对Cd处理下冬小麦根际土壤氧化还原酶活性、BIF及C/N的影响[J].应用与环境生物学报,2012,18(06):917.[doi:10.3724/SP.J.1145.2012.00917]
　JIA Xia,DONG Suiming,ZHOU Chunjuan.Effects of Low Doses of Pb on Rhizosphere Soil Oxidoreductase Activities, BIF, and C : N Ratio of Winter Wheat Seedlings Under Cd[J].Chinese Journal of Applied & Environmental Biology,2012,18(05):917.[doi:10.3724/SP.J.1145.2012.00917]
[3]王海斌** 叶江华 陈晓婷 贾小丽 孔祥海.连作茶树根际土壤酸度对土壤微生物的影响[J].应用与环境生物学报,2016,22(03):480.[doi:10.3724/SP.J.1145.2015.09019]
　WANG Haibin**,YE Jianghua,CHEN Xiaoting,et al.Effect on soil microbes of the rhizospheric soil acidity of tea tree continuous cropping*[J].Chinese Journal of Applied & Environmental Biology,2016,22(05):480.[doi:10.3724/SP.J.1145.2015.09019]
[4]张奇,王海斌,李立,等.田间直播下化感水稻抑草作用及其根际土壤微生物生理特性动态[J].应用与环境生物学报,2018,24(03):478.[doi:10.19675/j.cnki.1006-687x.2017.07002]
　ZHANG Qi,WANG Haibin,et al.Field tests to study the dynamics of weed inhibition of allelopathic rice and microbial physiological traits of rice rhizospheric soils[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):478.[doi:10.19675/j.cnki.1006-687x.2017.07002]
[5]王海斌,陈晓婷,丁力,等.不同树龄茶树根际土壤细菌多样性的T-RFLP分析[J].应用与环境生物学报,2018,24(04):775.[doi:10.19675/j.cnki.1006-687x.2017.10003]
　WANG Haibin,**,CHEN Xiaoting,et al.Using T-RFLP technology to analyze bacterial diversity in the rhizospheric soils of tea tree at different ages[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):775.[doi:10.19675/j.cnki.1006-687x.2017.10003]

更新日期/Last Update: 2018-10-25