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[1]冯殿宝,王维红,王燕杉,等.以黏土为载体的好氧颗粒污泥培养及其对番茄废水的处理[J].应用与环境生物学报,2019,25(01):199-205.[doi:10.19675/j.cnki.1006-687x.2018.03033]
　FENG Dianbao,WANG Weihong**,WANG Yanshan & SU Kuizu.Clay-cultured aerobic granular sludge and its use in the treatment of tomato-paste processing wastewater[J].Chinese Journal of Applied & Environmental Biology,2019,25(01):199-205.[doi:10.19675/j.cnki.1006-687x.2018.03033]
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以黏土为载体的好氧颗粒污泥培养及其对番茄废水的处理()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年01期
页码:
199-205
栏目:
研究论文
出版日期:
2019-02-25

文章信息/Info

Title:
Clay-cultured aerobic granular sludge and its use in the treatment of tomato-paste processing wastewater
作者:
冯殿宝;  王维红;  王燕杉;  苏馈足
1新疆农业大学水利与土木工程学院 乌鲁木齐 830052 2合肥工业大学土木与水利工程学院 合肥 230009
Author(s):
FENG Dianbao1;  WANG Weihong1**;  WANG Yanshan1 & SU Kuizu 2
1 College of Hydraulic and Civil Engineering, Xinjiang Agricultural University, Urumqi 830052, China 2 School of Civil Engineering, Hefei University of Technology, Hefei 230009, China
关键词:
番茄酱加工废水; 好氧颗粒污泥; 黏土; 高通量测序技术
Keywords:
tomato paste processing wastewater;  aerobic granular sludge;  clay;  high-throughput sequencing
分类号:
X703
DOI:
10.19675/j.cnki.1006-687x.2018.03033
摘要:
针对新疆番茄酱加工废水排放量大、有机物浓度高等问题，采用好氧颗粒污泥技术处理. 为了解黏土对于污泥好氧颗粒化进程的影响，在SBR反应器中接种絮体污泥并投加黏土，以人工合成番茄酱加工废水为基质培养好氧颗粒污泥，并采用扫描电镜、激光共聚焦（CLSM）、死活细菌染色以及高通量测序等技术表征活性污泥的颗粒化过程. 结果显示，在反应器中投加黏土运行20 d时获得平均粒径0.54 mm左右的好氧颗粒污泥. 颗粒污泥成熟后，污泥沉降性能较好，COD、NH4+-N、PO43--P的平均去除率分别达到90%、85%、40%以上. 扫描电镜下，颗粒污泥轮廓清晰，结构密实. CLSM显示，α-多糖、β-多糖和蛋白质作为胞外聚合物（EPS）的组成部分在颗粒中分布广泛，贯穿整个颗粒截面. 添加黏土培养的颗粒污泥死活细菌染色显示其死细菌数量积累程度更高，活细菌多位于外层并包裹着死细菌. 高通量测序结果显示，黏土投加后微生物多样性提高，Shannon指数由4.50增至4.79，动胶菌属和产黄菌属的相对丰度分别为37.87%和8.79%. 本研究表明投加黏土可促进好氧颗粒污泥形成，维持体系稳定运行及有机物降解效果主要源于微生物群落的共同作用. （图8 表1 参30）
Abstract:
The aerobic granular sludge technology was adopted to treat tomato-paste processing wastewater in Xinjiang because it receives high amount of discharge products and high concentration of organic matter. A sequencing batch reactor (SBR) was operated to investigate the effect of clay augmentation on aerobic granulation. Flocculent sludge and clay were fed into the synthetic tomato-paste processing wastewater contained in the SBR. The combined bio-analytical techniques of scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), live/dead staining, and high-throughput sequencing were used to characterize the granulation process of activated sludge. The results showed that the average particle size of the granules in the clay-added reactor was approximately 0.54 mm in 20 days. After maturation, the granular sludge had a good settling ability, and the average removal rate of COD, NH4+-N, and PO43--P reached 90%, 85%, and 40%, respectively. The SEM results showed that the granules had a clear outline and compacting structure. The CLSM results revealed that α-D-glucopyranose polysaccharide, β-D-glucopyranose polysaccharide, and protein, as components of extracellular polymeric substances (EPS), were widely distributed throughout the granule. The live/dead bacterial staining images of the clay-added granular sludge showed a high accumulation of dead bacteria. The live bacteria were distributed more closely towards the edge of the granule, while the dead bacteria were trapped inside. The high-throughput sequencing results showed that the addition of clay led to an increase in microbial diversity, with the Shannon index increasing from 4.50 to 4.79. The relative abundances of the aerobic bacterial genera Zoogloea and Flavobacterium were 37.87% and 8.79%, respectively. The study demonstrated that the addition of clay could accelerate the formation of aerobic granular sludge, and the maintenance of the stability of the system and degradation of organic matter mainly resulted from the combined action of microbial communities.

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更新日期/Last Update: 2019-02-25