|本期目录/Table of Contents|

[1]郭玉琼,王仲,朱晨,等.茶树CSD1基因及其启动子克隆与低温胁迫下的表达[J].应用与环境生物学报,2018,24(05):1122-1131.[doi:10.19675/j.cnki.1006-687x.2018.02021]
　GUO Yuqiong,WANG Zhong,ZHU Chen,et al.Cloning and expression of the copper/zinc-superoxide dismutase 1 gene and its promoter under low temperature stress in Camellia sinensis[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1122-1131.[doi:10.19675/j.cnki.1006-687x.2018.02021]
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茶树CSD1基因及其启动子克隆与低温胁迫下的表达
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年05期
页码:
1122-1131
栏目:
研究论文
出版日期:
2018-10-25

文章信息/Info

Title:
Cloning and expression of the copper/zinc-superoxide dismutase 1 gene and its promoter under low temperature stress in Camellia sinensis
作者:
郭玉琼; 王仲; 朱晨; 赵姗姗; 张舒婷; 常笑君; 李小桢; 傅海峰; 林玉玲; 赖钟雄
1福建农林大学园艺学院 福州 350002 2福建农林大学园艺植物生物工程研究所 福州 350002 3贺州学院食品与生物工程学院 贺州 542899
Author(s):
GUO Yuqiong1;  WANG Zhong1;  2;  ZHU Chen1;  2;  ZHAO Shanshan1;  ZHANG Shuting1;  2;  CHANG Xiaojun1;  2;  LI Xiaozhen1;  FU Haifeng1;  LIN Yuling1;  2 & LAI Zhongxiong1;  2**
1 College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2 Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China 3 College of Food and Biological Engineering, Hezhou University, Hezhou 542899, China
关键词:
茶树; CSD1基因; 低温胁迫; 表达分析
Keywords:
Camellia sinensis;  copper/zinc-superoxide dismutase 1 (CSD1) gene;  low-temperature stress;  expression analysis
分类号:
S571.1 : Q78
DOI:
10.19675/j.cnki.1006-687x.2018.02021
摘要:
低温寒害严重影响茶树的生长发育状况及成品茶品质，Copper/zinc-superoxide dismutase（CSD）基因在植物抗胁迫响应中起着关键作用. 为了解茶树CSD基因（CsCSD）响应低温胁迫的作用机制，以铁观音茶树叶片为供试材料，采用同源克隆结合RACE方法及染色体步移法，获得茶树CSD1基因的cDNA序列（GenBank登录号：KR078346）、gDNA序列及其启动子序列，并对其进行生物信息学分析，同时对低温胁迫处理下CsCSD1基因的表达模式也进行分析. 结果显示，茶树CsCSD1基因cDNA全长860 bp，完整的开放阅读框（ORF）长度为459 bp，共编码152个氨基酸；gDNA基因结构分析发现CsCSD1基因由6个外显子和5个内含子构成；CpG岛预测发现启动子区域内存在1个CpG岛，CpG长度为219 bp，GC含量为50.3%；CsCSD1启动子上还存在大量的顺式作用元件，包括光响应元件、激素响应元件、胁迫响应元件和其他响应元件；在低温胁迫下CsCSD1基因的表达模式显示，在低温胁迫前期，CsCSD1基因表达上调；随后CsCSD1基因的表达量不断下降. 本研究表明，CsCSD1基因能够响应低温胁迫并在胁迫的不同时期发挥不同的作用，推测与低温胁迫密切相关. （图7 表3 参51）
Abstract:
The growth and quality of tea are severely?affected by low temperature. Copper/zinc-superoxide dismutase (CSD) genes play crucial roles in the response of plants to stress. The objective of this study was to explore the response mechanism of CSD gene in tea (CsCSD) under low temperature stress. The leaves of Camellia sinensis “Tieguanyin” were used as raw material. The full-length cDNA (GenBank accession number: KR078346), gDNA, and promoter sequence of CsCSD1 were cloned using homology-based cloning, combined with RACE and chromosome walking. Moreover, the bioinformatic characteristics and expression patterns of CsCSD1 under low temperature stress were investigated. The results showed that the full-length cDNA of CsCSD1 was 860 bp long, containing a 459 bp open reading frame (ORF) which encodes a protein of 152 amino acids. The gDNA structural analysis found that the CsCSD1 gene was composed of 6 exons and 5 introns. In addition, the CpG island prediction found that there was only a CpG island in the promoter region. The length of the CpG island was 219 bp and the GC content was 50.3%. The CsCSD1 promoter also contained a large number of cis-acting elements, including light response elements, hormone response elements, stress response elements, and other response elements. Expression analysis revealed that the expression of CsCSD1 was up-regulated at the early stage of low temperature stress, and then the expression decreased continuously. This study showed that the CsCSD1 gene can respond to low temperature stress and play different roles in different periods of low temperature stress.

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更新日期/Last Update: 2018-10-25