|本期目录/Table of Contents|

 LI Yi,LI Qinfen,et al.Isolation, identification, and the biodegradation characteristics of a benzoylurea insecticides-degrading strain[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):928-933.[doi:10.19675/j.cnki.1006-687x.2017.09012]





Isolation, identification, and the biodegradation characteristics of a benzoylurea insecticides-degrading strain
1中国热带农业科学院环境与植物保护研究所 海口 5711012农业部儋州农业环境科学观测实验站 儋州 571737
LI Yi1 2 LI Qinfen1 2 DENG Xiao1 2 WU Dongming1 2 & WU Chunyuan1 2**
1 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China2 Experiment Station of Agricultural Environment Scientific Observation in Danzhou, Ministry of Agriculture, Danzhou 571737, China
benzoylurea insecticide isolation and identification of a degrading-strain biodegradation characteristics cloning of degrading gene
从农药厂活性污泥中分离筛选到一株可降解灭幼脲、除虫脲、氟铃脲的菌株,命名为M6. 经生理生化特征和16S rRNA基因序列分析,将其鉴定为无色杆菌属(Achromobacter sp.). 菌株M6可在48 h内降解91%以上初始浓度为100 mg/L的灭幼脲、除虫脲、氟铃脲;且可在不添加其他碳源的情况下,以这3种杀虫剂为唯一碳源生长. 选取菌株降解效果较好的灭幼脲为底物,研究其降解特性. 菌株M6降解灭幼脲时,对温度、pH值等培养条件适应范围较宽,降解灭幼脲的最适温度为30 ℃,最适pH为7.0;可耐受400 mg/L的灭幼脲. 通过对乙酰氨基酚变色和芳基酰胺酶基因克隆试验,初步确定菌株M6通过水解酰胺键降解灭幼脲、除虫脲、氟铃脲. 本研究得到了苯甲酰脲类杀虫剂的高效降解菌,可为其污染修复的开展提供资源和理论基础. (图8 参31)
A chlorbenzuron, diflubenzuron, and hexaflumuron-degrading bacterium strain M6, was isolated from the activated sludge of an insecticide factory. The strain was identified as Achromobacter sp. according to an analysis on the 16S rRNA gene sequences, morphological, and physiological characteristics. Strain M6 could degrade more than 91% of 100 mg/L chlorbenzuron, diflubenzuron, and hexaflumuron within 48 hours, which could act as the sole carbon source. Strain M6 showed more chlorbenzuron degradation at a temperature range between 25 and 40 ℃ and a pH range between 6.0 and 8.0. The optimal temperature and the initial pH of medium for chlorbenzuron degradation by strain M6 were 30 ℃ and 7.0, respectively; the maximum chlorbenzuron tolerated concentration of strain M6 was as high as 400 mg/L. Strain M6 hydrolyzed 4-acetaminophenol into a purple-red product. Moreover, an approximately 1.4 kb DNA fragment, which could be expressed into an amidase to degrade amide pesticides, was amplified from the genomic DNA of strain M6. The results preliminarily proved that 3 benzoylurea insecticides could be degraded because of strain M6 hydrolyzing their amide bonds. This study obtained a highly efficient degrading strain and provided new resources and valuable information on benzoylurea insecticide degradation.


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更新日期/Last Update: 2018-08-25