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[1]陶美,贺玉龙,王林,等.四环素降解菌的筛选及其降解特性[J].应用与环境生物学报,2018,24(02):384-389.[doi: 10.19675/j.cnki.1006-687x.2017.06040]
 TAO Mei,HE Yulong,WANG Lin,et al.Screening and degradation characteristics of a tetracycline-degrading bacterial strain[J].Chinese Journal of Applied & Environmental Biology,2018,24(02):384-389.[doi: 10.19675/j.cnki.1006-687x.2017.06040]
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四环素降解菌的筛选及其降解特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年02期
页码:
384-389
栏目:
研究简报
出版日期:
2018-04-25

文章信息/Info

Title:
Screening and degradation characteristics of a tetracycline-degrading bacterial strain
作者:
陶美贺玉龙王林王臣董世阳谭周亮李旭东
1西南交通大学地球科学与环境工程学院 成都 610031 2中国科学院环境与应用微生物重点实验室 成都 610041; 3中国科学院成都生物研究所,环境微生物四川省重点实验室 成都 610041
Author(s):
TAO Mei HE Yulong WANG Lin WANG Chen DONG Shiyang TAN Zhouliang LI Xudong
1 Southwest Jiaotong University, Faculty of Geosciences and Environmental Engineering, Chengdu 610031, China 2 Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 3 Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
关键词:
四环素克雷伯氏菌菌株筛选生物降解降解特性响应面法
Keywords:
tetracycline Klebsiella pneumoniae strain screening biodegradation degradation characteristic response surface methodology
分类号:
X523 : Q939.97
DOI:
10.19675/j.cnki.1006-687x.2017.06040
摘要:
四环素类抗生素在畜禽养殖业中的大量使用给生态环境带来了严重危害. 从活性污泥中筛选到一株对四环素具有良好降解能力的高效降解菌株,命名为TTC-1. 形态观察、革兰氏染色及16S rRNA序列分析表明该菌株为革兰氏阴性菌,并鉴定为克雷伯氏菌(Klebsiella pneumoniae). 采用单因素试验分别研究温度、初始pH、接种量、金属盐种类等因素对该菌株四环素降解效率的影响. 基于单因素试验结果,通过响应面优化该菌的四环素降解条件,拟合得到二次多项回归模型,确定了降解四环素的最佳条件为温度34.4 ℃,pH 7.22,MnSO4浓度0.32 g/L. 在最优条件下,预测四环素降解率为93.77%,验证值为94.26%,说明建立的模型具有较高的精度. 本研究表明TTC-1对四环素具有良好降解效果,有望为该菌在含四环素的实际废水生物强化处理过程中提供理论参考. (图4 表3 参24)
Abstract:
Tetracycline is widely used in livestock and poultry breeding industry, which can cause serious problems to the environment. Antibiotic pollution has become an important environmental issue. This study aimed to isolate and identify a well-functioning tetracycline-degrading bacteria strain from activated sludge and to investigate its optimum degradation conditions. The strain was identified through morphological features, Gram staining, and the sequence analysis of 16S rRNA. Furthermore, the temperature, initial pH of the medium, inoculation amount, and type of metallic salt were analyzed to investigate the tetracycline degradation performance of the isolated strain. Based on the single factor test, the method of response surface analysis was adopted to optimize the degradation condition. The strain was named TTC-1 and identified as Klebsiella pneumoniae. The optimum condition for tetracycline degradation was determined as follows: temperature of 34.4 °C, pH of 7.22, and MnSO4 concentration of 0.32 g/L. Under this optimum condition, the predicted tetracycline degradation rate was 93.77%, whereas the observed value was 94.26%. The experimental results showed that the proposed model had high accuracy. TTC-1 showed a good performance in degrading tetracycline, which can provide reference for the bacteria during the biological treatment of tetracycline containing wastewater.

参考文献/References:

1. 秦松岩, 李杭, 山丹, 张芹, 解永磊. 四环素类抗生素生产废水处理现状与研究进展[J]. 天津理工大学学报, 2016, 32 (2): 50-54 [Qin SY, Li H, Shan D, Zhang Q, Xie YL. The present situation and research progress in the treatment of tetracycline antibiotic manufacturing wastewater [J]. J Tianjin Univ Technol, 2016, 32 (2): 50-54]
2. Chee-Sanford JC, Mackie RI, Koike S, Krapac IG, Lin YF, Yannarell AC, Maxwell S, Aminov RI. Fate and transport of antibiotic residues and antibiotic resistance genes following land application of manure waste [J]. J Environ Qual, 2009, 38 (2): 1086-1108
3. 廖丹. 兽用抗生素在水环境中的残留特征及有害效应[J]. 热带农业工程, 2013, 37 (2): 11-14 [Liao D. Residue characteristics and harmful effects of typical veterinary antibiotics in aquatic environment [J]. Trop Agric Eng, 2013, 37 (2): 11-14]
4. 沈怡雯, 黄智婷, 谢冰. 抗生素及其抗性基因在环境中的污染、降解和去除研究进展[J]. 应用与环境生物学报, 2015, 21 (2): 181-187 [Shen YW, Huang ZT, Xie B. Advances in research of pollution, degradation and removal of antibiotics and antibiotic resistance genes in the environment antibiotic resistance genes in the environment [J]. Chin J Appl Environ Biol, 2015, 21 (2): 181-187]
5. Yang WB, Zheng FF, Lu YP, Xue XX, Li N. Adsorption interaction of tetracyclines with porous synthetic resins [J]. Ind Eng Chem Res, 2016, 50 (24): 13892-13898
6. Zhao C, Deng HP, Yuan L, Liu ZZ. Photodegradation of oxytetracycline in aqueous by 5A and 13X loaded with TiO2 under UV irradiation [J]. J Hazard Mater, 2010, 176 (1): 884-892
7. Dalmázio I, Almeida MO, Augusti R, Alves TMA. Monitoring the degradation of tetracycline by ozone in aqueous medium via atmospheric pressure ionization mass spectrometry [J]. J Am Soc Mass Spectr, 2007, 18 (4): 679-687
8. Zhang J, Giorno L, Drioli E. Study of a hybrid process combining PACs and membrane operations for antibiotic wastewater treatment [J]. Desalination, 2006, 194 (1-3): 101-107
9. 许晓玲, 李卫芬, 雷剑, 王敏奇. 四环素降解菌的选育、鉴定及其降解特性[J]. 农业生物技术学报, 2011, 19 (3): 549-556 [Xu XL, Li WF, Lei J, Wang MQ. Screening, identification and degradation characteristics of tetracycline-degrading strains [J]. Chin J Agric Biotechol, 2011, 19 (3): 549-556]
10. 马志强, 马玉龙, 谢丽, 张作义, 王敏. 微生物法降解药渣中残留四环素的试验研究[J]. 环境科学与技术, 2012, 35 (1): 46-49 [Ma ZQ, Ma YL, Xie L, Zhang ZY, Wang M. Experimental study on microbial degradation of tetracycline residues in antibiotic waste [J]. Environ Sci Technol, 2012, 35 (1): 46-49]
11. 冯福鑫, 许旭萍, 程群星, 韩永和. 四环素高效降解酵母菌Trichosporon mycotoxinivorans XPY-10降解特性[J]. 环境工程学报, 2013, 7 (12): 4779-4785 [Feng FX, Xu XP, Cheng QX, Han YH. Degradation characteristics of tetracycline hydrochloride by Trichosporon mycotoxinivorans XPY-10 [J]. Chin J Environ Eng, 2013, 7 (12): 4779-4785]
12. 张欣阳, 蔡婷静, 许旭萍. 一株高效四环素降解菌的分离鉴定及其降解性能研究[J]. 生物技术通报, 2015, 31 (1): 173-180 [Zhang XY, Cai TJ, Xu XP. Isolation and identification of a tetracycline-degrading bacterium and optimizing condition for tetracycline degradation [J]. Biotechnol Bull, 2015, 31 (1): 173-180]
13. 赵永斌. 3种四环素类抗生素降解菌的筛选及降解特性的研究[D]. 晋中: 山西农业大学, 2015 [Zhao YB. The selection of 3 tetracycline degrading bacteria and the character of isolation and degrading [D]. Jinzhong: Shanxi Agricultural University, 2015]
14. 黄建凤, 张发宝, 逄玉万, 黄巧义, 唐拴虎. 1株四环素降解菌的分离鉴定及降解特性研究[J]. 微生物学杂志, 2017, 37 (01): 50-56 [Huang JF, Zhang FB, Pang YW, Huang QY, Tang SH. Isolation, identification and characteristics of a tetracycline-degradation bacterium [J]. J Microbiol, 2017, 37 (01): 50-56]
15. Patil PD, Gude VG, Mannarswamy A, Cooke P, Munson-McGee S, Nirmalakhandan N, Lammers P, Deng S. Optimization of microwave-assisted transesterification of dry algal biomass using RSM [J]. Bioresour Technol, 2010, 102 (2): 1399-1405
16. Li W, Zhao LC, Sun YS, Lei FJ, Wang Z, Gui XB, Wang H. Optimization of pressurized liquid extraction of three major acetophenones from Cynanchum bungei using a Box-Behnken Design [J]. Int J Mol Sci, 2012, 13 (11): 14533
17. 陈茂霞, 王欢, 周后珍, 李杨, 谭周亮, 李旭东. 异养硝化-好氧反硝化菌HN-02的筛选及其特性[J]. 应用与环境生物学报, 2013, 19 (4): 688-693 [Chen MX, Wang H, Zhou HZ, Li Y, Tan ZL, Li XD. Screening and characteristics of heterotrophic nitrification-aerobic denitrification strain HN-02 [J]. Chin J Appl Environ Biol, 2013, 19 (4): 688-693]
18. 周德庆. 微生物学试验教程[M]. 2版. 北京: 高等教育出版社, 2006: 21-32 [Zhou DQ. Microbiology Experiment Tutorial [M]. 2nd ed. Beijing: Higher Education Press, 2006: 21-32]
19. Halling-S?rensen B, Lykkeberg A, Ingerslev F, Blackwell P, Tj?rnelund J. Characterisation of the abiotic degradation pathways of oxytetracyclines in soil interstitial water using LC-MS-MS [J]. Chemosphere, 2003, 50 (10): 1331-42
20. 王辉. Fe(Ⅱ/Ⅲ)对四环素类抗生素降解的影响及机制研究[D]. 北京: 北京交通大学, 2016 [Wang H. Effects and mechanisms of Fe(Ⅱ/Ⅲ) on the degradation of tetracycline antibiotics in water [D]. Beijing: Beijing Jiaotong University, 2016]
21. Hu GZ, Hu H, Liu BG, Yuan L, Liu JH, Pan YS, Wu H, Chen YX. Characterization of blaCTX-M gene in one Klebsiella pneumoniae isolate from sick chickens in China [J]. J Integr Agric, 2012, 11 (10): 1714-1720
22. Leski TA, Bangura U, Jimmy DH, Ansumana R, Lizewski SE, Stenger DA, Taitt CR, Vora GJ. Multidrug-resistant tet(X)-containing hospital isolates in Sierra Leone [J]. Int J Antimicrob Agents, 2013, 42 (1): 83-86
23. Pal RR, Khardenavis AA, Purohit HJ. Identification and monitoring of nitrification and denitrification genes in Klebsiella pneumoniae, EGD-HP19-C for its ability to perform heterotrophic nitrification and aerobic denitrification [J]. Funct Integr Genomic, 2015, 15 (1): 63-76
24. 刘杰伟, 马俊伟, 刘彦忠, 杨娅, 岳东北, 王洪涛. 克雷伯氏菌生产絮凝剂M-C11的培养优化及其在污泥脱水中的应用[J]. 环境科学, 2014, 35 (3): 1183-1190 [Liu JW, Ma JW, Liu YZ, Yang Y, Yue DB, Wang HT. Optimized cultivation of a bioflocculant M-C11 produced by Klebsiella pneumoniae and its application in sludge dewatering [J]. Environ Sci, 2014, 35 (3): 1183-1190]
25.

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更新日期/Last Update: 2018-04-25