|本期目录/Table of Contents|

[1]邹婷,刘唤唤,刘永胜,等.拟南芥新基因SI（stress insensitive）参与非生物胁迫应答[J].应用与环境生物学报,2018,24(02):328-334.[doi:10.19675/j.cnki.1006-687x.2017.05043]
　ZOU Ting,LIU Huanhuan,LIU Yongsheng,et al.A novel gene SI (stress insensitive) is involved in the response to abiotic stress in Arabidopsis[J].Chinese Journal of Applied & Environmental Biology,2018,24(02):328-334.[doi:10.19675/j.cnki.1006-687x.2017.05043]
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拟南芥新基因SI（stress insensitive）参与非生物胁迫应答()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年02期
页码:
328-334
栏目:
研究论文
出版日期:
2018-04-25

文章信息/Info

Title:
A novel gene SI (stress insensitive) is involved in the response to abiotic stress in Arabidopsis
作者:
邹婷; 刘唤唤; 刘永胜; 汪松虎
1 四川大学生命科学学院生物资源与生态环境教育部重点实验室，水利学与山区河流工程国家重点实验室 成都 6100642 中国科学院成都生物研究所 成都 6100413 中国科学院东南亚生物多样性研究中心 缅甸内比都 05282
Author(s):
ZOU Ting;  LIU Huanhuan ;  LIU Yongsheng ;  WANG Songhu
1Ministry of Education Key Laboratory for Bio-resource and Eco-environment, College of Life Sciences, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China2 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China3 Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences, Nay Pyi Taw 05282, Myanmar
关键词:
拟南芥; DUF1336; 瞬时转化; 脱落酸（ABA）; 非生物胁迫
Keywords:
Arabidopsis;  DUF1336;  transient expression;  abscisic acid (ABA);  abiotic stress
分类号:
Q945.78 : Q786
DOI:
10.19675/j.cnki.1006-687x.2017.05043
摘要:
非生物胁迫是影响农作物产量的主要因素，植物对非生物胁迫的应答机制一直是植物学研究的热点之一. 在拟南芥中克隆和鉴定了一个参与非生物胁应答的新基因SI（stress insensitive），该基因编码一个未知功能结构域的蛋白DUF1336，该家族蛋白结构域具有许多未知功能的假设植物蛋白C末端（约250个残基）. 在单子叶植物和双子叶植物中，SI蛋白质是保守的. 通过瞬时转化实验证明，该蛋白定位于细胞质膜. 实时定量PCR分析结果显示，SI基因在各种组织中组成型表达. 在花、果荚和种子中表达量相对较高. 非生物胁迫和脱落酸（ABA）可以增加SI基因的表达，SI基因在ABA、NaCl和冷处理后分别上调3.5倍、2.1倍和4.7倍. SI基因的T-DNA插入突变体（SALK_021811C）植株表现出对冷胁迫和高盐胁迫的抗性. 种子的萌发实验也表明，突变体种子可以在高盐胁迫条件下萌发，对ABA的抑制作用也不敏感. 本研究表明SI基因是植物抗逆性的负调控因子，可为提高农作物的遗传改良提供新的候选基因. （图5 参35）
Abstract:
Abiotic stresses are a major cause of loss of crop yield. The adaptation of plants to abiotic stress has been always a hotspot of botanical research. In this study, we cloned and characterized a novel gene SI (stress insensitive) involved in the response to abiotic stress in Arabidopsis. The SI protein has a new domain called DUF1336, and this family represents the C-terminus (approximately 250 residues) of a number of hypothetical plant proteins of unknown function. Furthermore, the SI protein is conserved among monocots and dicots. The subcellular localization analysis showed that the SI protein is localized in the plasma membrane. The quantitative RT-PCR analysis showed that the SI gene is constitutively expressed in various tissues. The expression of SI was relatively high in flowers, siliques, and seeds. Abiotic stress and abscisic acid (ABA) treatment can enhance the expression of SI gene, and SI transcripts were upregulated under ABA, NaCl, and cold treatments by 3.5, 2.1, and 4.7 folds, respectively. The T-DNA insertion mutant of SI gene (SALK 021811C) showed increased tolerance to cold and salt stresses. In addition, the seed germination experiments indicated that the seeds of SI mutant were insensitive to salt stress and ABA treatment. In conclusion, the results demonstrate that the SI gene plays a negative role in the tolerance of plants to abiotic stress and is a good candidate gene for genetic engineering to increase the tolerance of crop to abiotic stress.

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更新日期/Last Update: 2018-04-25