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[1]王广林,邓辉,聂丽,等.卵形孢球托霉Gongronella butleri NL-15对石灰岩的侵蚀机制[J].应用与环境生物学报,2018,24(02):374-378.[doi:10.19675/j.cnki.1006-687x.2017.05018]
　WANG Guanglin,DENG Hui,NIE Li,et al.Mechanism of limestone corrosion by Gongronella butleri NL-15[J].Chinese Journal of Applied & Environmental Biology,2018,24(02):374-378.[doi:10.19675/j.cnki.1006-687x.2017.05018]
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卵形孢球托霉Gongronella butleri NL-15对石灰岩的侵蚀机制()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年02期
页码:
374-378
栏目:
研究论文
出版日期:
2018-04-25

文章信息/Info

Title:
Mechanism of limestone corrosion by Gongronella butleri NL-15
作者:
王广林; 邓辉; 聂丽; 吴雁雯; 张金池
1皖西学院分析测试中心 六安 237012 2南京林业大学林学院 南京 210037
Author(s):
WANG Guanglin;  DENG Hui;  NIE Li;  WU Yanwen ;  ZHNAG Jinchi
1 Analytical and Testing Center of West Anhui University, Liu’an 237012, China 2 College of Forestry, Nanjing Forestry University, Nanjing 210037, China
关键词:
卵形孢球托霉; 有机酸; 乳酸; 岩石侵蚀; 客土喷播
Keywords:
Gongronella butleri;  organic?acid ;  lactic acid;  rock corrosion;  soil spray-sowing
分类号:
Q939.99
DOI:
10.19675/j.cnki.1006-687x.2017.05018
摘要:
客土喷播技术的关键是高效岩石侵蚀微生物菌株的利用，岩石侵蚀机制研究可为筛选此类微生物菌株提供理论依据. 选择从岩石表面分离纯化出的一株优势、高效石灰岩侵蚀菌Gongronella butleri NL-15，采用岩石粉发酵培养，研究菌株发酵不同时期发酵液的pH、主要离子浓度、有机酸含量及岩石颗粒的表面形貌特征. 结果显示：菌株发酵液的pH值与钙（Ca）、镁（Mg）离子含量存在显著的负相关性，对照的pH值为6.87，而15 d发酵液的pH值降低到5.12；对照的Ca、Mg离子含量分别为38.96、10.85 mg/L，而15 d发酵液的Ca、Mg离子分别升高到367.56、76.16 mg/L. 总有机酸和乳酸的含量随着发酵时间增加而增多，2、5、9、15 d的发酵液中总有机酸含量分别为3.43、5.40、6.63、7.26 mg/mL，乳酸含量分别是1.79、2.85、5.16、5.04 mg/mL. 菌丝体包裹岩石颗粒，插入其空穴，崩解岩石颗粒. 上述结果表明，菌株产生有机酸特别是乳酸导致岩石疏松化以及菌丝体生长穿插入疏松化的岩石内部，是其侵蚀石灰岩释放出Ca、Mg等离子的主要因素. （图3 表2 参19）
Abstract:
The key to soil spray-sowing technology is the use of the highly-effective rock-corroding strains, and the mechanism of rock corrosion will provide theoretical?references to screen the strains. To investigate the corrosion mechanism of limestone by microorganisms, the dominant and highly-effective limestone-corroding strain Gongronella butleri NL-15 was isolated and purified from the microorganisms on the rock surface. The concentration of calcium (Ca), magnesium (Mg), potassium, and phosphorus was analyzed by ICP. The pH of the fermentation broth of the strain at different intervals was measured using a precise pH instrument. The?content of organic?acids (critic, succinic, lactic, fumaric, acetic, and propanoic acids) and the scanning?electron?microscope feature of rock particles in the fermentation broth at different intervals were also analyzed by HPLC. The results showed that the concentration of Ca2+ and Mg2+ in the fermentation broth correlated negatively with the pH value (P < 0.01). The pH of control was 6.87, and that of the fermentation broth after 15 days dropped to 5.12. The concentration of Ca2+ and Mg2+ was 38.96 and 10.85 mg/L respectively, whereas that of the fermentation broth after 15 days increased to 367.56 and 76.16 mg/L respectively. The content of the total organic?acids and lactic acid increased with increase in fermentation time. The content of the total organic?acids on days 2, 5, 9, and 15 in the fermentation broth was 3.43, 5.40, 6.63, and 7.26 mg/mL, respectively, and the content of lactic acid was 1.79, 2.85, 5.16, and 5.04 mg/mL, respectively. The mycelium of the G. butleri NL-15 adhered to the surface of rock particles and threaded into unconsolidated rocks. Thus, the?organic?acids, especially lactic acid produced by the fungi G. butleri NL-15, caused unconsolidation of rock and improved the growth of fungi mycelium into the unconsolidated rock. This was the primary cause for limestone corrosion, and the release of Ca2+ and Mg2+ from the rock and its disintegration.

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更新日期/Last Update: 2018-04-25