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[1]秦澎,李津,辜运富,等.香菇生长发育过程中漆酶基因家族的转录表达[J].应用与环境生物学报,2018,24(02):379-383.[doi: 10.19675/j.cnki.1006-687x.2017.03010]
　QIN Peng,LI Jin,GU Yunfu,et al.Transcriptional expression profiles of the laccase gene family in different development stages of Lentinus edodes[J].Chinese Journal of Applied & Environmental Biology,2018,24(02):379-383.[doi: 10.19675/j.cnki.1006-687x.2017.03010]
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香菇生长发育过程中漆酶基因家族的转录表达()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年02期
页码:
379-383
栏目:
研究简报
出版日期:
2018-04-25

文章信息/Info

Title:
Transcriptional expression profiles of the laccase gene family in different development stages of Lentinus edodes
作者:
秦澎; 李津; 辜运富; 曾先富; 向泉桔
1四川农业大学资源学院微生物系 成都 611130 2成都市农林科学院园艺研究所 成都 611130
Author(s):
QIN Peng;  LI Jin;  GU Yunfu;  ZENG Xianfu ;  XIANG Quanju
1 Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu 611130, China 2 Institute of Horticulture, Chengdu Academy of Agricultural and Forestry Sciences, Chengdu 611130, China
关键词:
香菇; 漆酶; 菌丝体; 原基; 子实体; RT-qPCR
Keywords:
Lentinus edodes;  laccase;  mycelium;  primordium;  fruiting body;  RT-qPCR
分类号:
S646.120.1 : Q786
DOI:
10.19675/j.cnki.1006-687x.2017.03010
摘要:
香菇可分泌胞外漆酶降解木质素作为自身营养物质. 为探究不同漆酶基因在香菇生长发育过程中的不同作用，采用RT-qPCR方法分析10个漆酶基因在香菇4个不同发育阶段（菌丝体、原基、幼小子实体和成熟子实体）的转录表达情况. 结果显示，5个漆酶基因（Lelac1、Lelac3、Lelac5、Lelac8和Lelac9）的转录表达表现出差异性和发育阶段特异性，另外5个漆酶基因（Lelac2、Lelac4、Lelac7、Lelac10和Lelac11）只表现出差异性. Lelac2和Lelac3随着香菇的生长，其转录表达量持续上升，可能与成菇过程有关；Lelac1和Lelac7在菌丝体阶段大量表达，可能参与菌丝体的生长代谢；Lelac8在原基时期表达量显著高于其他时期，与原基分化有关. 本研究表明漆酶基因在香菇不同发育阶段的转录表达存在差异，结果可为进一步阐明漆酶在香菇生长发育阶段的作用奠定基础. （图3 表1 参19）
Abstract:
Laccase plays an important role in the nutritional metabolism and growth of Lentinus edodes. To explore the role of the laccase gene family in the growth and development of L. edodes, the transcriptional expression profiles of laccase genes in different developmental stages of this species were investigated. Transcriptional expression profiles of 10 laccase genes in four different developmental stages, mycelium, primordium, young fruiting body, and mature fruiting body, were analyzed using RT-qPCR. The results showed that different laccase genes exhibited specific or differential expression profiles across development stages. Five laccase genes (Lelac1, Lelac3, Lelac5, Lelac8, and Lelac9) showed specific or differential expression profiles, whereas the other five laccase genes (Lelac2, Lelac4, Lelac7, Lelac10, and Lelac11) showed differential expression profiles. The relative expression levels of Lelac2 and Lelac3 increased with L. edodes growth, implying their participation in fruiting processes. Lelac1 and Lelac7 were highly expressed in the mycelium stage, suggesting their function in mycelial growth and metabolism. Lelac8 displayed higher expression levels in the primordium stage than in other developmental stages, indicating it might be related to primordium differentiation. In conclusion, laccase genes showed differential transcriptional expression across developmental stages, and these results have provided a foundation for further research of laccase function in L. edodes.

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更新日期/Last Update: 2018-04-25