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 YANG Zhengzhong,WU Guang,JIN Wen,et al.Isolation, identification and characterization of an iprodione-degrading strain YJN-G[J].Chinese Journal of Applied & Environmental Biology,2017,23(01):164-168.[doi:10.3724/SP.J.1145.2016.03040]





Isolation, identification and characterization of an iprodione-degrading strain YJN-G
1南京农业大学生命科学学院 南京 210095 2河西学院农业与生物技术学院 张掖 734000
YANG Zhengzhong1 WU Guang1 JIN Wen1 CAO Li1 2 YAN Xin1 & HONG Qing1**
1College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China 2College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, China
iprodione biodegradation Microbacterium sp. metabolic pathway
为去除环境中异菌脲残留,从某农药厂废水处理系统的活性污泥中分离到一株异菌脲降解菌YJN-G,对其进行鉴定和降解特性分析. 通过形态特征、生理生化特性和16S rRNA基因序列相似性分析,将其初步鉴定为微杆菌属(Microbacterium sp.). 当接种量为5%时,菌株YJN-G在24 h内能够降解100 mg/L的异菌脲. 菌株YJN-G降解异菌脲的最适pH是7.0,最适温度为30-37 ℃. 通过对其降解异菌脲产物的质谱分析,确定其代谢产物为N-(3,5-二氯苯基)-2,4-二氧代咪唑烷和异丙基氨基甲酸;菌株能够利用异丙基氨基甲酸生长,但是不能进一步降解N-(3,5-二氯苯基)-2,4-二氧代咪唑烷. 菌株降解异菌脲的水解酶属于胞内酶. 本研究结果可为异菌脲污染环境的生物修复提供菌株资源和理论依据. (图7 表1 参18)
To clean up iprodione residues in the environment, a bacterial strain YJN-G capable of degrading iprodione was isolated from the activated sludge in the wastewater treatment system of a pesticide factory. Based on its physiological & biochemical characters and the similarity analysis of 16S rRNA gene sequence, strain YJN-G was primarily identified as Microbacterium sp. With the inoculation amount of 5%, strain YJN-G was able to degrade 100 mg L-1 iprodione within 24 hours, with optimum temperature of 30-37 °C and pH of 7.0. The metabolites were analyzed by mass spectrometry and identified as N-(3,5-dichlorophenyl)-2,4-dioxo-imidazolidin and isopropyl carbamic acid. Strain YJN-G could use isopropyl carbamic acid as the carbon source for growth, but could not further degrade N-(3,5-dichlorophenyl)-2,4-dioxo-imidazolidin. The hydrolase responsible for the degradation of iprodione was an endoenzyme. The result of this study provides strain resource and theoretical basis for bioremediation of iprodione-contaminated environment.


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更新日期/Last Update: 2017-02-25