|本期目录/Table of Contents|

[1]张华,李莹,刘静,等.基于定量蛋白质组学分析迭鞘石斛对肺癌细胞的抑制作用及机理[J].应用与环境生物学报,2017,23(01):100-104.[doi:10.3724/SP.J.1145.2016.02006]
 ZHANG Hua,LI Ying,et al.A quantitative proteomics study of the inhibitory effect of D. denneanum on lung tumor cells and its mechanism[J].Chinese Journal of Applied & Environmental Biology,2017,23(01):100-104.[doi:10.3724/SP.J.1145.2016.02006]
点击复制

基于定量蛋白质组学分析迭鞘石斛对肺癌细胞的抑制作用及机理()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年01期
页码:
100-104
栏目:
研究论文
出版日期:
2017-02-25

文章信息/Info

Title:
A quantitative proteomics study of the inhibitory effect of D. denneanum on lung tumor cells and its mechanism
作者:
张华李莹刘静何涛蒋伟淳泽胡亚东
1中国科学院成都生物研究所 成都 610041 2中国科学院大学 北京 100049
Author(s):
ZHANG Hua1 2 LI Ying1 2 LIU Jing1 HE Tao1 JIANG Wei1 CHUN Ze1** & HU Yadong1**
1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
迭鞘石斛肺癌细胞作用机理SILAC定量蛋白质组
Keywords:
D. denneanum lung cancer cells mechanism of anti-tumor SILAC quantitative proteomics
分类号:
R285.5
DOI:
10.3724/SP.J.1145.2016.02006
摘要:
以基于SILAC(Stable isotope labeling with amino acids)的定量蛋白质组学为主要研究方法,通过精确定量蛋白质含量并进行差异分析,从蛋白层面系统研究迭鞘石斛(Dendrobium denneanum)乙醇提取物对人肺癌细胞A549的影响. 结果发现迭鞘石斛乙醇提取物对A549细胞具有明显的抑制作用,其在降低细胞增殖速率的同时导致细胞发生死亡,24 h和48 h时的抑制率分别为35.05%和59.08%. 定量蛋白质组学实验发现迭鞘石斛乙醇提取物引起A549细胞中392个蛋白质的含量出现明显下调. 功能分析显示下调蛋白主要包括细胞物质和能量代谢相关蛋白,如RNA代谢与蛋白质合成、糖类代谢、脂类代谢、嘧啶与嘌呤代谢等. 同时大量与细胞抵抗内源及外界不利刺激和损伤相关的蛋白,如具有氧化还原、分子伴侣、DNA修复、蛋白降解与修复等功能的蛋白含量也出现明显的下调. 本研究通过定量蛋白质组学实验从全细胞蛋白层面系统解释迭鞘石斛的抑癌作用机理进行了,可促进石斛抗癌机理的进一步研究和石斛源抗癌新药的开发工作. (图5 表2 参18)
Abstract:
Dendrobium denneanum is shown to have significant anti-cancer effects, but their specific anti-cancer mechanism is still not understood in detail. Because of the complex composition of its extract, traditional research methods could not find out the anticancer mechanism and its targets systematically. To solve this problem, this research aimed to study the anticancer effect and mechanism of ethanol extract of D. denneanum based on quantitative proteomics. We used quantitative proteomics based on SILAC technique to investigate the inhibitory function of D. denneanum on lung tumor cell line A549 and the mechanism underlying such effect. The result showed that ethanol extract of D. denneanum not only significantly inhibited cell proliferation of A549 cell line, but also led to cell death. The inhibition rate reached 35.05% at 24 h and 59.08% at 48 h. Quantitative proteomics experiments found that 392 proteins of A549 cell line were down regulated by the ethanol extract of D. denneanum. Function analysis showed that the down-regulated proteins were mainly those involved in cellular material and energy metabolism, such as RNA metabolism and protein synthesis, carbohydrate metabolism, lipid metabolism, purine and pyrimidine metabolism; a large number of proteins with the function of resisting endogenous and external adverse stimuli and injuries including redox molecular chaperones, DNA repair, protein degradation and restoration, were also significantly down regulated. This research explains from the point of quantitative proteomics the cancer inhibition effect and its mechanism of D. denneanum, and lays foundation for future research and development of new anticancer drugs based on D. denneanum.

参考文献/References:

1 Guo P, Huang ZL, Yu P. Trends in cancer mortality in China: an update [J]. Ann Oncol Offic JEur Soc Med Oncol, 2012, 23 (10): 2755-2762
2 Ng TB, Liu J, Wong JH. Review of research on Dendrobium, a prized folk medicine [J]. Appl Microbiol Biotechnol, 2012, 93 (5): 1795-1803
3 Jae In S, Yu Jin K, Hae-Young Yl. Denbinobin, a phenanthrene from Dendrobium nobile, inhibits invasion and induces apoptosis in SNU-484 human gastric cancer cells [J]. Oncol Rep, 2011, 27 (3): 813-818
4 An-Chi T, Shiow-Lin P, Cho-Hwa L. Moscatilin, a bibenzyl derivative from the India orchid Dendrobrium loddigesii, suppresses tumor angiogenesis and growth in vitro and in vivo [J]. Cancer Lett, 2010, 292 (2): 163-170
5 Prasad R, Koch B. Antitumor activity of ethanolic extract of Dendrobium formosum in T-cell lymphoma: an in vitro and in vivo study [J]. Biomed Res Intern, 2014, 2014 (6): 753451-753451
6 罗傲雪, 宋关斌, 范益军. 迭鞘石斛抗肿瘤作用动物实验研究[J]. 四川大学学报自然科学版, 2005, 42 (6): 1281-1283 [Luo AX, Song GB, Fan YJ. The research on the inhibiting tumor effect of D. Denneanum in vivo [J]. J Sichuan Univ (Nat Sci Ed ), 2005, 42 (6): 1281-1283]
7 罗傲雪, 宋关斌, 淳泽, 秦建, 罗傲霜, 范益军, 何涛. 迭鞘石斛抗肿瘤作用研究[J]. 应用与环境生物学报, 2007, 13 (2): 184-187 [Luo AX, Song GB, Chun Z, Qin J, Luo AS, Fan YJ, He T. Inhibiting effect of tumor by Dendrobium denneanum. Chin J Appl Environ Biol, 2007, 13 (2): 184-187]
8 Christofk HR, Heiden MGV, Harris MH. The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth [J]. Nature, 2008, 452 (7184): 230-233
9 Yan G, Wei J, Lu G. Mouse embryonic fibroblasts from CD38 knockout mice are resistant to oxidative stresses through inhibition of reactive oxygen species production and Ca (2+) overload [J]. BBRC, 2010, 399 (2): 167-172
10 Stuart JA, Mayard S, Hashiguchi K. Localization of mitochondrial DNA base excision repair to an inner membrane-associated particulate fraction [J]. Nucleic Acids Res, 2005, 33 (12): 3722-3732
11 Rossi MR, Seema S, Garrett SH. Expression of hsp 27, hsp 60, hsc 70, and hsp 70 stress response genes in cultured human urothelial cells (UROtsa) exposed to lethal and sublethal concentrations of sodium arsenite [J]. EnvironHealth Perspect, 2002, 110 (12): 1225-1232
12 Torkild V, Berit D, Henrik Sahlin P. Uracil in DNA and its processing by different DNA glycosylases [J]. Philos Trans Royal Soc Lond, 2009, 364 (1517): 563-568
13 Rhee SG, Chae HZ, Kim K. Peroxiredoxins: a historical overview and speculative preview of novel mechanisms and emerging concepts in cell signaling [J]. Free Radical Biol Med, 2005, 38 (12): 1543-1552
14 Hyun AeW, Hee YS, Hae SD. Inactivation of peroxiredoxin I by phosphorylation allows localized H(2)O(2) accumulation for cell signaling [J]. Cell, 2010, 140 (4): 517-528
15 Egler RA, Fernandes EK, Sereika S. Regulation of reactive oxygen species, DNA damage, and c-Myc function by peroxiredoxin 1 [J]. Oncogene, 2005, 24 (54): 8038-8050
16 Joo Young H, Yunghee K, Jaeho J. Peroxiredoxin 3 is a key molecule regulating adipocyte oxidative stress, mitochondrial biogenesis, and adipokine expression [J]. Antioxid Redox Signal, 2012, 16 (3): 229-243
17 Wasinger VC, Zeng M, Yau Y. Current Status and Advances in Quantitative Proteomic Mass Spectrometry [J]. Int J Proteomics, 2013, 2013 (2013): 180605-180605
18 Matthias M. Functional and quantitative proteomics using SILAC [J]. Nat Rev Mol Cell Biol, 2006, 7 (12): 952-958

相似文献/References:

[1]范益军,淳泽,罗傲雪,等.迭鞘石斛中性多糖DDP1-1的体内免疫活性[J].应用与环境生物学报,2010,16(03):376.[doi:10.3724/SP.J.1145.2010.00376]
 FAN Yijun,CHUN Ze,LUO Aoxue,et al.In Vivo Immunomodulatory Activities of Neutral Polysaccharide (DDP1-1) from Dendrobium denneanum[J].Chinese Journal of Applied & Environmental Biology,2010,16(01):376.[doi:10.3724/SP.J.1145.2010.00376]
[2]潘红玫,陈斌,李甫,等.迭鞘石斛的化学成分[J].应用与环境生物学报,2012,18(03):378.[doi:10.3724/SP.J.1145.2012.00378]
 PAN Hongmei,CHEN Bin,LI Fu,et al.Chemical Constituents of Dendrobium denneanum[J].Chinese Journal of Applied & Environmental Biology,2012,18(01):378.[doi:10.3724/SP.J.1145.2012.00378]
[3]潘红玫,陈斌,李甫,等.迭鞘石斛的化学成分(Ⅱ)[J].应用与环境生物学报,2013,19(06):952.[doi:10.3724/SP.J.1145.2013.00952]
 PAN Hongmei,CHEN Bin,LI Fu,et al.Chemical Constituents from the Stems of Dendrobium denneanum (Ⅱ)[J].Chinese Journal of Applied & Environmental Biology,2013,19(01):952.[doi:10.3724/SP.J.1145.2013.00952]
[4]李婷婷,淳泽,罗傲雪,等.不同LED光质对迭鞘石斛生理及品质的影响[J].应用与环境生物学报,2017,23(05):934.[doi: 10.3724/SP.J.1145.2016.11040]
 LI Tingting,CHUN Ze,LUO Aoxue,et al.Effects of LED light quality on physiology and quality of Dendrobium denneanum[J].Chinese Journal of Applied & Environmental Biology,2017,23(01):934.[doi: 10.3724/SP.J.1145.2016.11040]
[5]李莹,刘静,张雪琴,等.石斛不同极性成分的提取及其对肺癌细胞的抑制作用[J].应用与环境生物学报,2018,24(01):13.[doi:10.19675/j.cnki.1006-687x.2016.08033]
 LI Ying,LIU Jing,ZHANG Xueqin,et al.Extraction of components with different polarities from $Dendrobium$ and their inhibitory action on lung cancer cells[J].Chinese Journal of Applied & Environmental Biology,2018,24(01):13.[doi:10.19675/j.cnki.1006-687x.2016.08033]

更新日期/Last Update: 2017-02-25