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[1]徐广飞,崔志松,栾晓,等.基于发光细菌法和鱼类暴露实验评价6种化学消油剂的遗传毒性[J].应用与环境生物学报,2017,23(01):146-151.[doi:10.3724/SP.J.1145.2016.01046]
 XU Guangfei,CUI Zhisong,**,et al.Genotoxicity evaluation of 6 chemical dispersants by luminescent bacteria test using Acinetobacter sp. RecA and fish exposure experiment using Oryzias melastigma[J].Chinese Journal of Applied & Environmental Biology,2017,23(01):146-151.[doi:10.3724/SP.J.1145.2016.01046]
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基于发光细菌法和鱼类暴露实验评价6种化学消油剂的遗传毒性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年01期
页码:
146-151
栏目:
研究论文
出版日期:
2017-02-25

文章信息/Info

Title:
Genotoxicity evaluation of 6 chemical dispersants by luminescent bacteria test using Acinetobacter sp. RecA and fish exposure experiment using Oryzias melastigma
作者:
徐广飞崔志松栾晓郑立
1青岛科技大学化工学院 青岛 266042 2国家海洋局第一海洋研究所海洋生态研究中心 青岛 266061 3青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室 青岛 266071
Author(s):
XU Guangfei1 CUI Zhisong2 3** LUAN Xiao2 & ZHENG Li2 3
1Chemical Engineering Institute of Qingdao University of Science & Technology, Qingdao 266042, China 2Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China 3Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China
关键词:
化学消油剂遗传毒性发光细菌海水青鳉彗星实验
Keywords:
chemical dispersant genotoxicity luminescent bacteria Oryzias melastigma comet assay
分类号:
X17 : X82
DOI:
10.3724/SP.J.1145.2016.01046
摘要:
为评价海洋溢油应急处置中所使用的消油剂的环境安全性,分别以发光细菌Acinetobacter sp. RecA和海水青鳉(Oryzias melastigma)幼鱼为受试生物,对6种化学消油剂的遗传毒性进行检测. 结果显示,采用发光细菌法仅检测到HLD-501(常规型)在浓度较高(500 mg/L和1 000 mg/L)时表现出一定水平的遗传毒性,其等效应的丝裂霉素C(MMC)浓度分别为0.039 mg/L和0.032 mg/L. 在彗星实验中,6种消油剂对海水青鳉幼鱼细胞的DNA损伤率从高到低依次为RS-II(浓缩型)、RS-I(浓缩型)、HLD-501(常规型)、富肯-2(常规型)、RS-I(常规型)和微普(常规型),然而HLD-501(常规型)处理的3级损伤率最高,其遗传毒性最大. 综上,采用两种不同营养级的受试生物对6种化学消油剂遗传毒性检测结果的相关度较好;本研究可为消油剂用于处理实际海洋溢油污染的环境安全性评价提供参考. (图3 表2 参28)
Abstract:
Chemical dispersants are widely used in emergency response of marine oil spills. However, their genotoxicity information is relatively scarce. This research aimed to evaluate the environmental safety of chemical dispersants used for rapid removal and cleaning up of marine oil spills. We used luminescent bacteria test (LBT) based on Acinetobacter sp. RecA combined with fish exposure experiment based on marine medaka (Oryzias melastigma) to detect the genotoxicity of 6 chemical dispersants. In LBT, the 500 mg/L and 1 000 mg/L of chemical dispersant HLD-501 exhibited genotoxicity of 0.039 mg/L and 0.032 mg/L of mitomycin C (MMC), respectively. In addition, the DNA damage ratio of O. melastigma by the 6 chemical dispersants in the comet assay was in the order of concentrate type RS-II > concentrate type RS-I > conventional type HLD-501 > conventional type Fuken-2 > conventional type RS-I > conventional type Weipu. However, HLD-501 resulted in the most serious DNA damage (level 3), being the most genotoxic among the 6 dispersants. The result of these two methods for genotoxicity detection fitted well with each other. This study could provide a useful reference for environmental safety evaluation of the chemical dispersants used in cleaning up of the marine oil spills.

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更新日期/Last Update: 2017-02-25