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[1]张 晶 孙照勇** 钟小忠 黄玉莲 谭 力 汤岳琴 木田建次.奶牛粪条垛式模拟堆肥腐熟度及微生物群落结构变化[J].应用与环境生物学报,2016,22(03):423-429.[doi:10.3724/SP.J.1145.2015.11030]
 ZHANG Jing,SUN Zhaoyong**,ZHONG Xiaozhong,et al.Maturity and microbial community structure dynamics during simulated windrow composting of solid fraction of dairy manure*[J].Chinese Journal of Applied & Environmental Biology,2016,22(03):423-429.[doi:10.3724/SP.J.1145.2015.11030]
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奶牛粪条垛式模拟堆肥腐熟度及微生物群落结构变化()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年03期
页码:
423-429
栏目:
研究论文
出版日期:
2016-06-25

文章信息/Info

Title:
Maturity and microbial community structure dynamics during simulated windrow composting of solid fraction of dairy manure*
作者:
张 晶 孙照勇** 钟小忠 黄玉莲 谭 力 汤岳琴 木田建次
四川大学建筑与环境学院 成都 610065
Author(s):
ZHANG Jing SUN Zhaoyong** ZHONG Xiaozhong HUANG Yulian TAN Li TANG Yueqin & KIDA Kenji
College of Architecture and Environment, Sichuan University, Chengdu 610065, China
关键词:
奶牛粪污压榨残渣条垛式堆肥翻堆微生物群落
Keywords:
Composting is an important technology for resource utilization of dairy manure. Composting with the solid fraction (SF) of manure slurry without adding bulking materials can reduce the processing volume and hereby reduce the composting cost. This is a par
分类号:
X713
DOI:
10.3724/SP.J.1145.2015.11030
摘要:
为降低堆肥成本、促进资源化利用和集约化养殖场奶牛粪污处理,采用小型堆肥反应器,研究利用奶牛粪污压榨残渣进行无辅料添加条垛式堆肥的可行性. 堆肥过程中监测物料温度和各种理化指标的变化,并跟踪解析微生物群落. 条垛式模拟堆肥共持续75 d,肥堆温度维持50 ℃以上达10 d,水分含量逐渐降低. pH在开始阶段略有增加后最终降低到约8.0. 有机物降解率在堆肥前30 d迅速增加,而后逐渐增加到57.8%,C/N随着有机物的降解降低到9.75. NH4+在堆肥进行20 d后显著降低,由于硝化作用NO3?逐渐增加,最终产品中未检测到NH4+,NO3?含量达到2 208.8 mg/kg(干重). 堆肥结束时物料的电导率为2 250.8 μS/cm,低于文献推荐限值3 000 μS/cm;成熟堆肥的种子发芽指数达到120.4%. 高通量测序结果显示,微生物群落结构在堆肥前期发生了显著变化,堆肥后期则保持相对稳定,氨氧化细菌Nitrosococcus和硝化细菌Nitrolancea、Nitrospira进行了NH4+向NO3?的转化,堆肥19 d后硝化细菌占总细菌的比例达2%以上. 综上,条垛式堆肥适合处理奶牛粪渣,可在无辅料添加情况下达到理想的堆肥效果,成熟堆肥质量优良. (图10 表1 参25)
Abstract:
Composting is an important technology for resource utilization of dairy manure. Composting with the solid fraction (SF) of manure slurry without adding bulking materials can reduce the processing volume and hereby reduce the composting cost. This is a particularly important technology for manure slurry management on intensive dairy farms. In this study, the feasibility of simulated windrow composting (non-aeration) of solid fraction of dairy manure without adding any bulking materials was evaluated in lab scale. Simulated windrow composting was conducted in a lab-scale reactor. During the composting process, turning was conducted once each 4 days at the first 4 weeks and thereafter once for every 10 days. The pile temperature, moisture, electrical conductivity, organic matter degradation efficiency, nitrification occurrence and germination index were investigated to evaluate the maturity and stability of material during the composing process. The microbial community dynamics was monitored by high-throughput sequencing. The simulated windrow composting lasted for 75 days. The pile temperature maintained above 50 °C up to 10 days and the moisture content decreased during the composting process. The pH increased at the first stage and then decreased to about 8.0. The organic matter degradation efficiency increased sharply during the first 30 days, and then increased gradually to 57.8% after 75 days of static composting. The C/N ratio first increased and then decreased to 9.75 after 30 days. NH4+ was not detected after 20 days and the NO3? content reached to 2208.8 mg/kg-dry matter in the end product. The final electrical conductivity and germination index was 2250.8 μS/cm and 120.4%, respectively. High-throughput sequencing results showed that the structure of microbial community changed significantly during the early stage, but kept stable at the later stage of composting process. NH4+ was oxidized to NO3? by ammonia-oxidizing bacterium Nitrosococcus and NO2?-oxidizing bacteria Nitrolancea and Nitrospira. The ratio of nitrification bacteria to total bacteria increased to >2% after 19 days. The results indicated that windrow composting is suitable for treating the solid fraction of dairy manure without adding bulking materials, and can achieve highly matured compost.

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更新日期/Last Update: 2016-06-25