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[1]伍思宇 周志如 尤 青 张文飞 徐 达 李特伦 姚 琦 张起畅 吴红萍 王锐萍?.海南东寨港红树林聚磷菌的筛选及其特性?[J].应用与环境生物学报,2016,22(03):397-401.[doi:10.3724/SP.J.1145.2015.11026]
　WU Siyu,ZHOU Zhiru,YOU Qing,et al.Screening and characterization of soil phosphate accumulating bacteria from Dongzhaigang mangrove wetlands in Hainan, China*[J].Chinese Journal of Applied & Environmental Biology,2016,22(03):397-401.[doi:10.3724/SP.J.1145.2015.11026]
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海南东寨港红树林聚磷菌的筛选及其特性?()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年03期
页码:
397-401
栏目:
研究论文
出版日期:
2016-06-25

文章信息/Info

Title:
Screening and characterization of soil phosphate accumulating bacteria from Dongzhaigang mangrove wetlands in Hainan, China*
作者:
伍思宇 周志如 尤 青 张文飞 徐 达 李特伦 姚 琦 张起畅 吴红萍 王锐萍?
热带动植物生态学省部共建教育部重点实验室，海南师范大学生命科学学院 海口 571158
Author(s):
WU Siyu;  ZHOU Zhiru;  YOU Qing;  ZHANG Wenfei;  XU Da;  LI Telun;  YAO Qi;  ZHANG Qichang;  WU Hongping & WANG Ruiping**
Ministry of Education Key Laboratory for Tropical Animal and Plant Ecology, College of Life Sciences, Hainan Normal University, Haikou 571158, China
关键词:
水体富营养化; 红树林; 聚磷菌; 菌株筛选; 不动杆菌属
Keywords:
water eutrophication;  mangrove;  phosphorus accumulating organisms;  screening;  Acinetobacter
分类号:
X172
DOI:
10.3724/SP.J.1145.2015.11026
摘要:
海南东寨港红树林自然保护区生态环境与聚磷菌的聚磷过程好氧-厌氧交替环境高度一致. 为获得高效聚磷菌，本研究随机采集该区域134份淤泥样品，通过BCIP蓝白斑筛选法、异染颗粒和PHB颗粒染色以及测定聚磷能力相结合的方法进行筛选. 总共获得185株初筛菌株，其中42株菌的聚磷能力达20%以上，最高可达69.5%，有效聚磷菌株分离率为22.7%. 利用Biolog微生物鉴定系统和16S rRNA基因序列分析鉴定菌株，发现所筛聚磷菌多为不动杆菌属（Acinetobacter），也有芽孢杆菌属（Bacillus）、寡养单胞菌属（Stenotrophomonas）和假单孢菌属（Pseudomonas），并且表现出一定的差异性. 本研究表明东寨港红树林具有丰富的聚磷微生物资源，可为修复磷富营养化水体恢复生态平衡提供有效的候选菌种. （图3 表3 参28）
Abstract:
Dongzhaigang Mangrove Forest Nature Reserve, located in northeastern Hainan Island, is the major mangrove wetlands in China. The mangroves are evergreen trees that grow along the tropical or sub-tropical tidal shores or river estuaries. At high tide, they are totally submerged or only their tops are visible; when the tide recedes, they show themselves as dense thickets. The mangroves are periodically submerged by seawater, which have the same eco-environment of accumulation of large quantities of polyphosphate within their cells and the removal of phosphorus by Phosphorus Accumulating Organisms (PAOs). Thus, PAOs are selectively enriched in the bacterial community within the activated sludge of mangrove wetlands. In the present study, we screened 134 sludge samples isolated from the mangrove wetlands in Dongzhaigang and obtained 185 isolates of PAOs through the methods of BCIP blue-and white-colored screening, Albert staining and Nile Blue staining, which determined if the polyP or polyhydroxybutyrate (PHB) is on accumulation. The results showed a phosphorus removal rate of 42 PAOs as higher than 20%, and the maximum removal efficiency for phosphorus higher than 80%. Biolog phenotype microarrays and 16S rDNA sequences analysis were conducted to identify the isolates of PAOs. The result showed the populations of PAOs constituted by the bacterial genus of Acinetobacter sp., Bacillus sp., Stenotrophomonas sp., Pseudomonas sp. genera etc., with Acinetobacter being dominant in the entire communities. In our study, Dongzhaigang Mangrove Forest Nature Reserve is rich in PAO strains resources with good diversity. In this study, PAOs isolated from Dongzhaigang Mangrove Forest Nature Reserve are important in enhanced biological phosphorus removal and can be a candidate strain for preventing eutrophication in water bodies.

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更新日期/Last Update: 2016-06-25