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[1]李勇昊 张晓月 赵心清** 白凤武.青霉菌发酵生产β-葡萄糖苷酶培养基优化及纤维素酶可溶性诱导物制备[J].应用与环境生物学报,2016,22(03):377-381.[doi:10.3724/SP.J.1145.2015.09015]
 LI Yonghao,ZHANG Xiaoyue,ZHAO Xinqing** & BAI Fengwu.Medium optimization for β-glucosidase production from Penicillium sp. and preparation of soluble inducer*[J].Chinese Journal of Applied & Environmental Biology,2016,22(03):377-381.[doi:10.3724/SP.J.1145.2015.09015]
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青霉菌发酵生产β-葡萄糖苷酶培养基优化及纤维素酶可溶性诱导物制备()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年03期
页码:
377-381
栏目:
研究论文
出版日期:
2016-06-25

文章信息/Info

Title:
Medium optimization for β-glucosidase production from Penicillium sp. and preparation of soluble inducer*
作者:
李勇昊1 张晓月1 赵心清2** 白凤武1
1大连理工大学生命科学与技术学院 大连 116024 2上海交通大学生命科学技术学院 上海 200240
Author(s):
LI Yonghao1 ZHANG Xiaoyue1 ZHAO Xinqing2** & BAI Fengwu12
1School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China 2School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
关键词:
β-葡萄糖苷酶青霉响应面优化转糖苷反应可溶性诱导物槐糖纤维素酶
Keywords:
β-glucosidase Penicillium response surface optimization transglycosylation reaction soluble inducer sophorose cellulase
分类号:
TQ353 : TQ925
DOI:
10.3724/SP.J.1145.2015.09015
摘要:
在纤维素酶生产过程中,常用的固体诱导物存在着诱导效率低、传质阻力大和不易于流加培养等局限性,所以利用β-葡萄糖苷酶催化葡萄糖生产槐糖等可溶性诱导物进行纤维素酶发酵具有重要意义. 为实现β-葡萄糖苷酶的低成本高效生产,首先利用本实验室分离的β-葡萄糖苷酶生产菌Penicillium sp. YH02为产酶菌株,利用响应面法对麸皮、麦草和微晶纤维素3个参数浓度进行优化,优化后β-葡萄糖苷酶酶活提高15.03%. 其次,利用菌株YH02所产的β-葡萄糖苷酶,以高浓度葡萄糖为底物进行转糖苷反应,合成诱导里氏木霉(Trichodema reesei)产纤维素酶的可溶性诱导物. 结果表明,以10 g/L该可溶诱导物为碳源时,里氏木霉 Rut-C30在48 h时滤纸酶活比未进行催化反应的葡萄糖对照高24.9倍. 离子色谱分析结果表明,高浓度葡萄糖经过菌株YH02分泌的β-葡萄糖苷酶催化后产生具有诱导能力的槐糖、龙胆二糖和纤维二糖. 本研究实现了β-葡萄糖苷酶的高效生产并成功制备了可溶性诱导物,为降低纤维素酶生产成本提供了参考. (图4 表1 参22)
Abstract:
Production of biofuels and bio-based chemicals from lignocellulosic materials have received worldwide attention. However, high cost cellulase production is one of the major bottlenecks for commercial production. Compared with insoluble cellulose materials, employment of soluble inducer could avoid the high mass transfer barrier in the bioreactor and difficulty in feeding. Therefore, development of efficient and low cost inducer is of great importance for low cost cellulase production. Low cost soluble inducer can be prepared by transglycosylation reaction using β-glucosidase. To achieve low cost and efficient β-glucosidase production, Penicillium sp. YH02 isolated by our lab was used as a producer strain, and wheat bran, barley grass and microcrystalline cellulose in the fermentation medium were further optimized for β-glucosidase production by response surface methodology. The β-glucosidase production was increased by 15.03% after optimization. Moreover, glucose-disaccharide mixture (GDM) was prepared using β-glucosidase produced by Penicillium sp. YH02, which was used as carbon source and inducer for cellulase production by Trichoderma reesei Rut C30. Filter Paper Activity (FPA) reached 1.12 IU/mL when GDM was supplied at the concentration of 10 g/L, which was 24.9 times higher compared with that using glucose. Sophorose, gentiobiose and cellobiose were detected in GDM analyzed by ion chromatography, among which sophorose is known as the most efficient soluble inducer for cellulase production of T. reesei. The results in this study benefit further development of low cost inducer for reducing the production cost of cellulase.

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更新日期/Last Update: 2016-06-25