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[1]张明明 万青青 张克俞 熊 亮 白凤武,赵心清**.过表达分支酸歧化酶编码基因ARO7对酿酒酵母抑制物耐受性的影响*[J].应用与环境生物学报,2016,22(02):201-205.[doi:10.3724/SP.J.1145.2015.09013]
 ZHANG Mingming,WAN Qingqing,ZHANG Keyu,et al.Effect of overexpression of chorismate mutase encoding gene ARO7 on theinhibitor tolerance of Saccharomyces cerevisiae[J].Chinese Journal of Applied & Environmental Biology,2016,22(02):201-205.[doi:10.3724/SP.J.1145.2015.09013]
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过表达分支酸歧化酶编码基因ARO7对酿酒酵母抑制物耐受性的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年02期
页码:
201-205
栏目:
研究论文
出版日期:
2016-04-25

文章信息/Info

Title:
Effect of overexpression of chorismate mutase encoding gene ARO7 on theinhibitor tolerance of Saccharomyces cerevisiae
作者:
张明明1 万青青2 张克俞2 熊 亮1 白凤武1 2 赵心清2**
1大连理工大学生命科学与技术学院 大连 1160242上海交通大学生命科学技术学院 上海 200240
Author(s):
ZHANG Mingming1 WAN Qingqing2 ZHANG Keyu2 XIONG Liang1 BAI Fengwu1 2 & ZHAO Xinqing2**
1School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China2State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
关键词:
纤维素乙醇菊芋秸秆酿酒酵母芳香族氨基酸ARO7乙酸酵母菌环境胁迫耐受性
Keywords:
cellulosic ethanol Jerusalem artichoke stalks Saccharomyces cerevisiae aromatic amino acid ARO7 acetic acidyeast stress tolerance
分类号:
TQ920.1
DOI:
10.3724/SP.J.1145.2015.09013
摘要:
利用纤维素原料生产乙醇一直是国内外研究的热点,但纤维素预处理过程产生的弱酸、酚类和糠醛等抑制物对酿酒酵母细胞生长和乙醇发酵具有抑制作用,因此,提高酿酒酵母细胞的胁迫耐受性是提高纤维素乙醇发酵效率的重要手段之一. 对芳香族氨基酸代谢途径中分支酸歧化酶基因ARO7的过表达对酿酒酵母在胁迫条件下细胞生长和乙醇发酵性能的影响进行研究. 结果显示,过表达ARO7的重组菌株在含有5.0 g/L乙酸的平板中生长优于对照菌株;在含有5.0 g/L乙酸的发酵培养基中进行乙醇发酵,过表达ARO7的重组菌株的发酵效率高于对照菌株,在菊芋秸秆水解液中ARO7过表达重组酵母菌株乙醇得率由对照的0.44 g/g提高到0.47 g/g葡萄糖,乙醇生产强度提高了22.38%. 以上表明,ARO7的过表达可提高酿酒酵母在抑制物存在条件下纤维素乙醇的发酵效率. (图4 表3 参25)
Abstract:
Fuel ethanol production using lignocellulosic materials has attracted widespread attention. However, inhibitorycompounds (e.g. weak acid, phenol and furfural) released from the pretreatment process exert inhibition on yeast cell growthand ethanol fermentation. Therefore, improvement of inhibitors tolerance of Saccharomyces cerevisiae benefits efficientcellulosic ethanol production. In this work, chorismate mutase encoding gene ARO7 which participates in aromatic aminoacid biosynthesis was overexpressed in S. cerevisiae, the cell growth and ethanol production of the recombinant strain in thepresence of inhibitory compounds were evaluated. It was found that the ARO7 overexpressing strain grew better than thecontrol strain in the presence of 5.0 g/L acetic acid. Similar result was found when ethanol fermentation was performed with 5.0g/L acetic acid addition. In addition, ethanol yield of the ARO7 overexpressing strain was improved from 0.44 g/g to 0.47 g/gglucose, and the ethanol productivity increased 22.38% when compared with the control strain using Jerusalem artichoke stalkshydrolysate. This work demonstrated that overexpression of ARO7 would be a feasible strategy to increase cellulosic ethanolproduction efficiency in the presence of inhibitors.

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更新日期/Last Update: 2016-04-25