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[1]梅 泽 许科伟 汤玉平 顾 磊 杨 帆 殷 波 刘 和 **.人工微宇宙环境下油气指示微生物的异常特征?[J].应用与环境生物学报,2016,22(03):415-422.[doi:10.3724/SP.J.1145.2015.09008]
 MEI Ze,XU Kewei,TANG Yuping,et al.The abnormal characteristics of oil and gas indicator microorganisms under the artificial environment of micro universe*[J].Chinese Journal of Applied & Environmental Biology,2016,22(03):415-422.[doi:10.3724/SP.J.1145.2015.09008]
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人工微宇宙环境下油气指示微生物的异常特征?()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年03期
页码:
415-422
栏目:
研究论文
出版日期:
2016-06-25

文章信息/Info

Title:
The abnormal characteristics of oil and gas indicator microorganisms under the artificial environment of micro universe*
作者:
梅 泽1 许科伟2 汤玉平2 顾 磊2 杨 帆2 殷 波2 刘 和 **
1江南大学环境与土木工程学院 无锡 214122 2中国石化石油勘探开发研究院无锡石油地质研究所 无锡 214151
Author(s):
MEI Ze 1 XU Kewei2 TANG Yuping2 GU Lei2 YANG Fan2 YIN Bo2 & LIU He1**
1School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China 2Wuxi Research Institute of Petroleum Geology, Exploration and Production Research Institute, SINOPEC, Wuxi 214151, China
关键词:
微生物勘探微宇宙16S rRNA高通量测序气态烃
Keywords:
microbial prospecting microcosm 16S rRNA high-throughout sequencing gaseous hydrocarbon
分类号:
Q939.99 : TE357.9
DOI:
10.3724/SP.J.1145.2015.09008
摘要:
为了解烃类对微生物的作用机制,通过人工微宇宙装置进行实验室水平模拟,结合培养法和分子生物学检测技术,以典型含油气区块土壤样品中的油气指示微生物为研究对象,探讨在模拟微渗漏条件下,一定驯化周期内不同轻烃组分驯化下油气指示微生物的数量变化和群落结构演替特征. 结果显示,在微量轻烃(1 × 10-8)持续驯化下,土壤中油气指示微生物数量发生了不同程度的增长,甲烷驯化组中甲烷氧化菌的菌落数量增长了76.5%,pmoA基因丰度增幅达180.0%;丁烷驯化组中丁烷氧化菌的菌落数量增长了212.0%,bmoX基因丰度增幅达285.0%. 16S rRNA高通量测序进一步显示,在模拟微渗漏(1 × 10-8)的条件下,油气指示微生物属于低丰度种群,其中Methylococcaceae(甲基球菌)、Methylophilaceae(嗜甲基菌)、Methylocystis(甲基孢囊菌)等微生物与甲烷正相关,Rhodosprillaceae(红螺菌)、Pseudonocardia(伪诺卡式菌)等微生物与丁烷正相关,其他的一些硝化微生物Nitrospira(硝化螺旋菌)、Xanthomonas(黄单胞菌)等则与烃类负相关. 本研究深化了关于气态烃对油气微生物作用机制的认识,可为油气微生物勘探技术提供新思路. (图6 表2 参33)
Abstract:
Microorganisms have been an important branch of surface oil and gas geochemical exploration. However, little is known about the function mechanism of hydrogen to microorganisms. This study adopted the incubation method and molecular biology to study the changes in the quantity and community structure of oil and gas hydrocarbon indicator microorganisms. The soil samples were taken from typical block and acclimated in micro-seepage (1 × 10-8) condition. The results showed that the quantity of oil and gas indicators all increased to different degrees. In groups acclimated by methane, the colonies of methane oxidizing bacteria increased by 76.5%, and the abundance of pmoA increased by 180.0%. In groups acclimated by butane, the colonies of butane oxidizing bacteria increased by 212.0%, and the abundance of bmoX increased by 285.0%. High-throughput sequencing further revealed that the oil and gas indicator microorganisms were low abundant population, with Methylococcaceae, Methylophilaceae, and Methylocystis (gas indicator bacteria) positively correlated with methane, Rhodospirillaceae, Pseudonocardia, and Methylobacter (oil indicator bacteria) positively correlated with butane, and other Nitrospira and Xanthomonas negatively correlated with hydrogen. This study intensified the understanding of function mechanisms about gaseous hydrocarbon to oil and gas microorganisms, to provide new ideas for oil and gas microorganisms exploration technology.

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更新日期/Last Update: 2016-06-25