|本期目录/Table of Contents|

[1]廖萍,刘茂柯,张瀚能,等.铬污染区糙野青茅内生耐铬细菌筛选及其促生能力[J].应用与环境生物学报,2015,21(06):1025-1031.[doi:10.3724/SP.J.1145.2015.07008]
 LIAO Ping,LIU Maoke,ZHANG Hanneng,et al.Chromium-resistant endophytic bacteria from Deyeuxia scabrescens (Griseb.) in Chromium contaminated area: isolation, screening and plant growth promoting[J].Chinese Journal of Applied & Environmental Biology,2015,21(06):1025-1031.[doi:10.3724/SP.J.1145.2015.07008]
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铬污染区糙野青茅内生耐铬细菌筛选及其促生能力()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年06期
页码:
1025-1031
栏目:
环境污染生态及生物治理和修复专栏
出版日期:
2015-12-25

文章信息/Info

Title:
Chromium-resistant endophytic bacteria from Deyeuxia scabrescens (Griseb.) in Chromium contaminated area: isolation, screening and plant growth promoting
作者:
廖萍 刘茂柯 张瀚能 赵翀 杨雅琳 张小平 张金羽 赵珂
1四川农业大学资源学院微生物系 成都 611130 2四川省农科院水稻高粱研究所 泸州 646000
Author(s):
LIAO Ping LIU Maoke ZHANG Hanneng ZHAO Chong YANG Yalin ZHANG Xiaoping ZHANG Jinyu ZHAO Ke
1Department of Microbiology, College of Resource and Environment, Sichuan Agricultural University, Chengdu 611130, China 2Rice and Sorghum Institute, Sichuan Academy of Agricultural Sciences, Luzhou 646000, China
关键词:
糙野青茅内生细菌耐铬促生
Keywords:
Deyeuxia scabrescens (Griseb.) endophytic bacteria chromium resistant plant growth promoting
分类号:
X172
DOI:
10.3724/SP.J.1145.2015.07008
文献标志码:
A
摘要:
以铬污染区糙野青茅[Deyeuxia scabrescens (Griseb.)]为研究对象,从中分离耐重金属铬的内生菌,获得对重金属铬有强耐受性和具有促生功能的细菌,为微生物与植物联合修复重金属污染提供菌种资源. 采用LB培养基,以涂布和撒接两种分离方法获得内生细菌,通过内生细菌在不同铬浓度培养基的生长状况来判断其对于重金属铬的耐受性,初筛耐受性强的菌株培养于含铬LB液体培养基培养后测定其OD值,以验证其对铬的耐受性. 将耐铬能力突出的菌株接种于含铬LB液体培养基培养后测定培养基中的残留铬含量,初探其去铬能力,并分析耐铬菌株多样性和产吲哚乙酸IAA、产铁载体、溶磷、降解纤维素等促生能力. 结果显示:从糙野青茅中共分离得到33株内生细菌,其中27株表现出较强的铬耐受性,5株细菌的铬耐受浓度高达1 000 μg/mL. 2株菌在铬浓度为300 μg/mL的处理下培养48 h后去铬率分别为79.55%和75.27%. 经16S rDNA序列测定,内生细菌属于Bacillus、Staphylococcus、Terribacillus、Ochrobacterium、Ignatzschineria、Alcaligenes 6个属. 24株菌(88.89%)具有产IAA能力,18株菌(66.67%)具有产铁载体能力,10株菌(37.04%)具有溶磷能力,13株菌(48.15%)具有降解纤维素能力. 本研究表明,铬污染区植物内生细菌在铬污染土壤的生物修复中具有很大潜力.
Abstract:
This research aimed to isolate the chromium-resistant bacterial endophyte strains and to evaluate their chromium resistance and plant growth promoting traits. LB medium and two isolate approaches were employed to isolate endophytic bacteria from Deyeuxia scabrescens (Griseb.) growing in chromium contaminated soil. In order to analyze the chromium resistance of the endophytic bacteria, the isolates were inoculated on medium of different chromium concentration. To verify the chromium stress resistance and removal ability, the isolates with strong chromium resistance in preliminary screening were measured for the OD value in LB liquid medium containing different concentration of chromium. The diversity and plant growth promoting activity of the endophytes were also analyzed. Out of the 33 endophytic bacteria isolated from Deyeuxia scabrescens (Griseb.), 27 strains could grow on media with chromium concentration greater than 500 ug/mL, with 5 isolates showing chromium stress resistance up to 1000 μg/mL. Additional experimental revealed the high chromium removal rate of SCAUE9002 (79.55%) and SCAUE9006 (75.27%) in LB medium (300 mg Cr6+/L, 48 h incubation). Phylogenetic analysis based on 16S rDNA sequences showed that these isolates belonged to 6 species, including Bacillus, Staphylococcus, Terribacillus, Ochrobacterium, Ignatzschineria and Alcaligenes. In terms of plant growth promoting ability, indole-3-acetic acid (IAA) production was detected in 24 strains (72.73%), Siderophore production was positive in 18 strains (54.54%), 10 strains (30.3%) of the test isolates showed ability to solubilize phosphate, and 13 strains produced cellulases (39.40%). Our research suggested that bacterial endophytes from chromium contaminated area are promising in bioremediation for chromium contaminated soils.

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备注/Memo

备注/Memo:
国家863计划项目(2013AA102802-05)资助 Supported by the National High-tech R&D Program (863 Program, 2013AA102802-05)
更新日期/Last Update: 2016-01-05