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[1]王文秀,马靖宇,朱祎捷,等.一株海洋锰氧化真菌的分离、鉴定及其对Mn(II)的氧化特性[J].应用与环境生物学报,2015,21(06):1032-1036.[doi:10.3724/SP.J.1145.2015.07001]
 WANG Wenxiu,MA Jingyu,ZHU Yijie,et al.Identification of a manganese-oxidizing fungus isolated from marine sediment and its Mn(II) oxidation characteristics[J].Chinese Journal of Applied & Environmental Biology,2015,21(06):1032-1036.[doi:10.3724/SP.J.1145.2015.07001]
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一株海洋锰氧化真菌的分离、鉴定及其对Mn(II)的氧化特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年06期
页码:
1032-1036
栏目:
环境污染生态及生物治理和修复专栏
出版日期:
2015-12-25

文章信息/Info

Title:
Identification of a manganese-oxidizing fungus isolated from marine sediment and its Mn(II) oxidation characteristics
作者:
王文秀 马靖宇 朱祎捷 崔鸿鹏 魏士平
1中国地质大学(北京)海洋学院 北京 100083 2Faculty of Science, University of Waterloo, Waterloo N2L 3G1, Canada
Author(s):
WANG Wenxiu MA Jingyu ZHU Yijie CUI Hongpeng WEI Shiping
1School of Marine Sciences, China University of Geosciences, Beijing, 10083, China 2Faculty of Science, University of Waterloo, Waterloo, N2L 3G1, Canada
关键词:
锰氧化真菌Ascomycete锰氧化锰去除锰氧化物
Keywords:
manganese-oxidizing fungus Ascomycete Mn oxidation Mn removal manganese oxides
分类号:
X172
DOI:
10.3724/SP.J.1145.2015.07001
文献标志码:
A
摘要:
从北戴河海洋沉积物中共分离出120株真菌,经筛选得到一株锰氧化能力较强的菌株PSA-107h. 经ITS测序并比较,该菌株与Ascomycete中的菌株有较高的同源性. 随着Mn2+浓度的增高,其对锰的氧化能力逐渐增强,但在高浓度Mn2+时,其生长会受到一定的抑制. 另外,发现PSA-107h在pH 5.0-9.0范围内都能对锰进行氧化,且在pH 6.0时对锰氧化的程度较高. 通过显微镜观察,发现真菌对锰的氧化主要在胞间进行,且随着菌丝生长时期的延长,锰氧化程度逐渐升高. PSA-107h在7 d内对液体中1 mmol/L的Mn2+的去除效率可达88.6%. 本研究为微生物除锰提供了良好的菌种资源.
Abstract:
Microbial organisms play an important role in oxidation of Mn2+ in the natural environment. In this research we isolated a total of 120 fungal strains from the sediment collected from Beidaihe and identified out of them a manganese-oxidizing strain PSA-107h. ITS sequence analysis showed that it was closely affiliated to Ascomycota sp. Experiment of its growth on media containing different manganese concentrations showed that the strain PSA-107h had greater manganese oxidation in higher manganese concentration, but very high manganese concentration would inhibit its growth. Manganese oxidation by PSA-107h could occur in a wide range of pH from 5.0 to 9.0, with the optimal pH of 6.0. Microscopy analysis revealed that the manganese oxidation was intercellular and becoming stronger with older mycelium. In liquid medium, the manganese removal of PSA-107h could reach 88.6%. This strain has great potential in industrial scale manganese removal from contaminated water.

参考文献/References:

1 叶伟. 环境中锰污染对居民健康的影响[J]. 环境与健康杂志, 1989, 6 (4): 43-46.
2 孟佑婷, 郑袁明, 张丽梅, 贺纪正.环境中生物氧化锰的形成机制及其与重金属离子的相互作用[J]. 环境科学, 2009, 30 (2): 574-582 [Meng YT, Zheng YM, Zhang LM, He JZ. Formation and reactions of biogenic manganese oxides with heavy metals in environment [J]. Environ Sci, 2009, 30 (2): 574-582]
3 张林静, 张琼, 郑袁明, 郑明兰. 环境修复中锰氧化物与变价重金属交互作用的研究进展[J]. 环境科学学报, 2013, 33 (6): 1519-1526 [Zhang LJ, Zhang Q, Zheng YM, Zheng ML, Interaction of manganese oxides with multiple valences heavy metals in environmental remediation [J]. Acta Sci Circumst, 2013, 33 (6): 1519-1526]
4 张震, 李林, 刘凡. 细菌氧化锰的分子机制[J]. 微生物学报, 2011, 51 (2): 170-177 [Zhang Z, Li L, Liu F. Molecular mechanism of bacterial manganese (II) oxidation-a review [J]. Acta Microbiol Sin, 2011, 51 (2): 170-177]
5 Gadd GM, Rhee YJ, Stephenson K, Wei Z. Geomycology: metals, actinides and biominerals [J]. Environ Microbiol Rep, 2012, 4 (3): 270-296
6 梁金松, 柏耀辉, 胡承志, Jefferson W, 曲久辉. 锰生物氧化的研究进展及在水处理中的应用[J]. 应用与环境生物学报, 2013, 19 (1): 11-19 [Liang JS, Bai SH, Hu CZ, Jefferson W, Qu JH. Research progress of biogenic manganese oxides and application potential in water treatment process [J]. Chin J Appl Environ Biol, 2013, 19 (1): 11-19]
7 张杰, 李冬, 陈立学, 杨宏. 地下水除铁除锰机理与技术的变革[M]. 自然科学进展, 2005, 15 (4): 433-438
8 杨伟红, 张震, 李林, 张忠明, 刘凡. 铁锰结核土壤锰氧化细菌多样性及新菌属分析[J]. 土壤, 2012, 44 (4): 606-612 [Yang WH, Zhang Z, Li L, Zhang ZM, Liu F. Identification of Mn (II)-oxidizing bacteria and novel genera from stratified Fe-Mn nodule forming soils [J]. Soils, 2012, 44 (4): 606-612]
9 张璐, 李婷婷, 王芳, 许旭萍. 锰氧化细菌的分离鉴定及其锰氧化特性的分析[J]. 微生物学通报, 2011, 38 (3): 328-332 [Zhang L, Li TT, Wang F, Xu XP. Isolation and identification of manganese-oxidizing bacterium and its manganese oxidation characteristics [J]. Microbiol China, 2011, 38 (3): 328-332]
10 秦松岩, 马放, 黄鹏. 自然水体中铁-锰氧化细菌的研究[J]. 环境科学学报, 2008, 29 (6): 1649-1654 [Qin SY, Ma F, Huang P. Ferrous-Manganese oxidizing bacteria from the nature water [J]. Environ Sci, 2008, 29 (6): 1649-1654]
11 Ehrlich HL. Bacteriology of manganese nodules II. Manganese oxidation by cell-free extract from a manganese nodule bacterium [J]. Appl Microbiol, 1968, 16 (2): 197-202
12 Emerson S, Kalhorn S, Jacobs L, Tebo BM, Nealson KH, Rosson RA. Environmental oxidation rate of manganese (II): bacterial catalysis [J]. Geochim Cosmochim Acta, 1982, 46 (6): 1073-1079
13 金圣圣, 贺纪正, 郑袁明, 孟佑婷, 张丽梅. 生物氧化锰矿物对几种重金属的吸附作用[J]. 环境科学学报, 2009, 29 (1): 132-139 [Jin SS, He JZ, Zheng YM, Meng YT, Zhang LM. Adsorption of heavy metals by biogenic manganese oxides [J]. Acta Sci Circumst, 2009, 29 (1): 132-139]
14 齐巍, 王向东, 蒋文举, 刘小燕, 李霞. 铁、锰氧化细菌在环境污染治理中的应用[J]. 环境污染治理技术与设备, 2004, 5 (6): 76-79 [Qi W, Wang XD, Jiang WJ, Liu XY, Li X. The application of ferromanganese-oxidizing bacteria in environmental pollution control [J]. Techn Equip for Environ Pollut Control, 2004, 5 (6): 76-79]
15 Tipping E, Thompson DW, Davison W. Oxidation products of Mn (II) in lake waters [J]. Chem Geol, 1984, 44 (4): 359-383
16 Mandernack KW, Post J, Tebo BM. Manganese mineral formation by bacterial spores of the marine Bacillus, strain SG-1: evidence for the direct oxidation of Mn (II) to Mn (IV) [J]. Geochim Cosmochim Acta, 1995, 59 (21): 4393-4408
17 Tebo BM, Johnson HA, McCarthy JK, Templeton AS. Geomicrobiology of manganese (II) oxidation [J]. Trends Microbiol, 2005, 13 (9): 421-428
18 姚远, 周志华, 许旭萍. 铁锰氧化菌的筛选及其生物学特性研究[J]. 福建师范大学学报: 自然科学版, 2009, 25 (6): 100-104 [Yao Y, Zhou ZH, Xu XP, Heng L, Li M. The selection of Fe2+ removing and Mn2+ removing bacteria and the study of biological characteristics [J]. J Fujian Norm Univ: Nat Sci Ed, 2009, 25 (6): 100-104]
19 刘凡, 冯雄汉, 陈秀华, 邱国红, 谭文峰, 贺纪正. 氧化锰矿物的生物成因及其性质的研究进展[J]. 地学前缘, 2008, 15 (6): 66-73 [Liu F, Feng XH, Chen XH, Qiu GH, Tan WF, He JZ. Advances in the study of biological genesis of manganese oxide minerals and their characteristics [J]. Earth Sci Frontiers, 2008, 15 (6): 66-73]
20 王芳, 许旭萍, 郑晓丹, 黄海飞. 锰细菌氧化锰的影响因素研究[J]. 福建师范大学学报: 自然科学版, 2010, 26 (2): 111-114 [Wang F, Xu XP, Zheng XD, Huang HF. Influencing factor of manganese oxidation of manganese bacterium [J]. J Fujian Norm Univ: Nat Sci Ed, 2010, 26 (2): 111-114]
21 Timonin MI, Illman WI, Hartgerink T. Oxidation of manganous salts of manganese by soil fungi [J]. Can J Microbiol, 1972, 18 (6): 793-799
22 Thompson IA, Huber DM, Guest CA, Schulze DG. Fungal manganese oxidation in a reduced soil [J]. Environ Microbiol, 2005, 7 (9): 1480-1487
23 Santelli CM, Webb SM, Dohnalkova AC, Hansel CM. Diversity of Mn oxides produced by Mn (II)-oxidizing fungi [J]. Geochim Cosmochim Acta, 2011, 75 (10): 2762-2776
24 Tebo BM, Clement BG, Dick GJ. Biotransformations of manganese, manual of environmental microbiology [J]. Am Soc Microbiol, 2007 (3): 1223-1238
25 Hansel CM, Zeiner CA, Santelli CM, Webb SM. Mn (II) oxidation by an ascomycete fungus is linked to superoxide production during asexual reproduction [J]. Proc Nat Acad Sci, 2012, 109 (31): 12621-12625
26 Tang Y, Zeiner CA, Santelli CM, Hansel CM. Fungal oxidative dissolution of the Mn (II)‐bearing mineral rhodochrosite and the role of metabolites in manganese oxide formation [J]. Environ Microbiol, 2013, 15 (4): 1063-1077
27 Miyata N, Tani Y, Maruo K, Tsuno H, Sakata M, Iwahori K. Manganese (IV) oxide production by Acremonium sp. strain KR21-2 and extracellular Mn (II) oxidase activity [J]. App Environ Microbiol, 2006, 72 (10): 6467-6473
28 Sasaki K, Matsuda M, Hirajima T, Takano K, & Konno H. Immobilization of Mn (II) ions by a Mn-oxidizing fungus Paraconiothyrium sp.-like strain at neutral pHs [J]. Mat Trans, 2006, 47 (10): 2457-2461
29 王文明, 邵宗泽, 王革娇. 一株海洋锰氧化细菌对Mn (II) 的氧化去除研究[J]. 湖北农业科学, 2009, 48 (1): 85-87 [Wang WM, Shao ZZ, Wang GJ Mn (II) oxidation and removing by a manganese-oxidizing bacterium from deep sea [J]. Hubei Agric Sci, 2009, 48 (1): 85-87]

备注/Memo

备注/Memo:
大学生创新创业训练计划教学实验室开放项目(国家级)(201411415067)资助 Supported by the Open Project of Teaching Laboratory of National Training Program of Innovation and Entrepreneurship for Undergraduate students (201411415067)
更新日期/Last Update: 2016-01-05