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[1]秦汉,秦家亮,黄国印,等.常温氢氧化钠预处理芦苇酶解发酵产油[J].应用与环境生物学报,2016,22(01):120-126.[doi:10.3724/SP.J.1145.2015.05012]
　QIN Han,QIN Jialiang,HUANG Guoyin,et al.Cellulosic biofuels production from alkali-pretreated phragmites at room temperature[J].Chinese Journal of Applied & Environmental Biology,2016,22(01):120-126.[doi:10.3724/SP.J.1145.2015.05012]
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常温氢氧化钠预处理芦苇酶解发酵产油()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年01期
页码:
120-126
栏目:
研究论文
出版日期:
2016-02-25

文章信息/Info

Title:
Cellulosic biofuels production from alkali-pretreated phragmites at room temperature
作者:
秦汉;  秦家亮;  黄国印;  刘海霞;  刘博;  史红霞;  李宪玲;  乔代蓉;  曹毅
四川大学生命科学学院微生物与代谢工程四川省重点实验室 成都 610065
Author(s):
QIN Han;  QIN Jialiang;  HUANG Guoyin;  LIU Haixia;  LIU Bo;  SHI Hongxia;  LI Xianling;  QIAO Dairong & CAO Yi**
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610065, China
关键词:
芦苇; 氢氧化钠; 常温; 响应面; 微生物油脂
Keywords:
reed;  sodium hydroxide;  room temperature;  response surface method;  microbial oil
分类号:
TK63 : TQ920.1
DOI:
10.3724/SP.J.1145.2015.05012
摘要:
在室温（28 ℃）下对氢氧化钠预处理芦苇（Phragmites australis）条件进行单因素和响应面设计优化，对酶解的条件进行单因素优化，对酶解液成分进行HPLC分析，利用未经任何脱毒处理的酶解液发酵产油并与配制培养基对比，通过GC-MS对油脂脂肪酸组成进行分析. 结果表明：最佳预处理条件为氢氧化钠质量分数3.4%，预处理时间16 h，液固比20:1 mL/g，在初始酶解条件下酶解得到酶解液还原糖浓度为26.32 g/L；最佳酶解的条件为pH = 4.5，温度45 ℃，液固比10:1 mL/g，酶解时间48 h，纤维素酶和纤维二糖酶酶液添加量均为30 μL/g，得到酶解液还原糖浓度为40.01 g/L；经HPLC分析，酶解液中葡萄糖、木糖、阿拉伯糖和半乳糖比例分别为70.27%、19.83%、5.08%和4.82%；通过隐球酵母（Cryptococcus podzolicus）ZWY-2-3发酵产油，其生物量、油脂产量和油脂含量在4 d时达到最大，分别为8.63 g/L、2.56 g/L和29.68%，其与同等糖浓度的对照组配制作培养基相当. 本研究表明，芦苇是生产微生物油脂的潜在生物质原料，具有广阔的应用前景. （图4 表8 参26）
Abstract:
Phragmites australis are widely distributed in China and have a large biomass yield, which make it a kind of economical material for the production of biofuels. This paper aimed to study the possibility of biofuels production from phragmites pretreated by alkali at ambient temperature. RSM and single-factor method was used to optimize the pretreatment and enzymatic hydrolysis conditions of phragmites. HPLC was used to analyze the composition of the enzymatic hydrolysate. Fatty acid composition of fermented lipid with different culture medium types was analyzed by GC-MS. The results showed the best pretreatment conditions as NaOH mass fraction of 3.4%, the time for 16 h, the liquid-solid ratio of 20:1 with the maximum concentration of sugar under the conditions as 26.32 g/L. The optimum hydrolysis conditions were pH = 4.5, temperature 45 oC, liquid-solid ratio 10:1 mL/g, time 48 h, the addition amount of cellulase and cellobiase 30 μL/g. The maximum concentration of reducing sugar was 40.01 g/L under this condition. HPLC results showed that enzymatic hydrolysate contained glucose and xylose, arabinose and galactose, with a ratio of 70.27% : 19.83% : 5.08% : 4.82%. The fermentation with enzymatic liquids of biomass during the first 4 days got a maximum biomass of 8.403 g/L, oil yields of 2.463 g/L and oil content of 29.70% with little difference with the control group. The microbial lipids fermented by synthetic medium and enzymatic liquids were analyzed and the results showed that the grease was mainly oleic acid. The ingredients of oil fermented by the enzymatic liquids and the artificial medium are basically the same. These results indicate the feasibility of producing microbial oil from Phragmites australis.

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更新日期/Last Update: 2016-02-25