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[1]黄婷,冉永红,罗世惠,等.红千层精油的抗氧化活性及其对中华按蚊幼虫的杀虫活性测定[J].应用与环境生物学报,2015,21(06):1086-1089.[doi:10.3724/SP.J.1145.2015.04058]
　HUANG Ting,RAN Yonghong,LUO Shihui,et al.Antioxidant capacity and larvicidal activity of Callistemon rigidus essential oil[J].Chinese Journal of Applied & Environmental Biology,2015,21(06):1086-1089.[doi:10.3724/SP.J.1145.2015.04058]
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红千层精油的抗氧化活性及其对中华按蚊幼虫的杀虫活性测定()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年06期
页码:
1086-1089
栏目:
研究论文
出版日期:
2015-12-25

文章信息/Info

Title:
Antioxidant capacity and larvicidal activity of Callistemon rigidus essential oil
作者:
黄婷;  冉永红;  罗世惠;  司风玲;  闫振天;  陈斌;  何正波
重庆师范大学昆虫与分子生物学研究所 重庆 401331
Author(s):
HUANG Ting;  RAN Yonghong;  LUO Shihui;  SI Fengling;  YAN Zhentian;  CHEN Bin;  HE Zhengbo
Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China
关键词:
红千层; 精油; 抗氧化能力; 中华按蚊; 杀幼虫活性
Keywords:
Callistemon rigidus;  essential oil;  antioxidant capacity;  Anopheles sinensis;  larvicidal activity
分类号:
Q946.85
DOI:
10.3724/SP.J.1145.2015.04058
文献标志码:
A
摘要:
为充分开发利用红千层（Callistemon rigidus R.Br.）植物资源，采用水蒸汽蒸馏法提取了红千层叶的精油（CrEO），测定了CrEO的总抗氧化能力；以人工合成抗氧化剂二丁基羟基甲苯（BHT）和丁基羟基茴香醚（BHA）作为阳性对照，测定了其对羟基（OH）自由基、1,1-二苯基-2-三硝基苯肼（DPPH）自由基的清除能力；并采用浸液法测试了CrEO对中华按蚊四龄幼虫的毒杀效果. 结果显示，CrEO的总抗氧化活性强于BHT，但弱于BHA，总抗氧化活性随浓度升高呈现显著增强的趋势. CrEO对DPPH自由基的清除率达到50%时的质量浓度（IC50）为6.452 mg/mL，对羟基自由基的IC50值小于1.0 × 10-5 mg/mL. 在毒杀实验中，CrEO对中华按蚊四龄幼虫的24 h毒杀LC50（半致死浓度）值为293.31 mg/L. 本研究结果表明，CrEO具有较强的抗氧化活性，对中华按蚊幼虫具有一定的毒杀活性，具备进一步开发成为天然的抗氧化剂和植物源杀虫剂的潜力.
Abstract:
In order to fully exploit and utilize the plant resources of Callistemon rigidus, we extracted essential oil from its leaves (CrEO) by steam distillation. The total antioxidant capacity, hydroxyl free radicals and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals scavenging of CrEO were measured using butylated hydroxytoluene (BHT) and butyl hydroxyanisole (BHA) as positive controls. Meanwhile, the larvicidal activity of CrEO against the larvae of Anopheles sinensis was evaluated by immersion method. The results indicated that CrEO had strong antioxidant ability. The total antioxidant capacity of CrEO was higher than that of BHT but lower than BHA, with the total antioxidant activity increased with the concentration. In addition, CrEO had good free radical scavenging activity. The half maximal inhibitory concentration (IC50) against DPPH radicals was 6.452 mg/mL, and the IC50 value against OH radical was less than 1.0 × 10-5 mg/mL. In addition, results of the 24 h mosquito larvicidal assay showed that CrEO had certain toxicity against larvae of A. sinensis with LC50 value of 293.310 ?g/mL. These findings suggested that CrEO can be a promising natural antioxidant and botanical mosquito control agent.

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相似文献/References:

[1]蒲自连,梁健.淡黄杜鹃植物挥发油化学成分的研究[J].应用与环境生物学报,1999,5(04):371.
　PU Zilian,et al..Chemical constituents of the essential oil from Rhododendron flavidum[J].Chinese Journal of Applied & Environmental Biology,1999,5(06):371.

备注/Memo

备注/Memo:
国家自然科学基金项目（31572332）、重庆市优秀人才支持计划、“两江学者”计划专项经费资助 Supported by the National Natural Science Foundation of China (31572332), the Program for Chongqing Excellent Talents in University, and the Par-Eu Scholars Program
更新日期/Last Update: 2016-01-05