|本期目录/Table of Contents|

[1]习海玲,刘昌财,问县芳,等.卤代烷烃脱卤酶及其对芥子气的降解研究进展[J].应用与环境生物学报,2015,21(05):842-847.[doi:10.3724/SP.J.1145.2015.04028]
 XI Hailing,LIU Changcai,WEN Xianfang,et al.Advances in research on haloalkane dehalogenases and its sulfur mustard degradation function[J].Chinese Journal of Applied & Environmental Biology,2015,21(05):842-847.[doi:10.3724/SP.J.1145.2015.04028]
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卤代烷烃脱卤酶及其对芥子气的降解研究进展()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年05期
页码:
842-847
栏目:
综述
出版日期:
2015-10-25

文章信息/Info

Title:
Advances in research on haloalkane dehalogenases and its sulfur mustard degradation function
作者:
习海玲 刘昌财 问县芳 陈立坤
国民核生化灾害防护国家重点实验室 北京 102205
Author(s):
XI Hailing LIU Changcai WEN Xianfang CHEN Likun
State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China
关键词:
卤代烷烃脱卤酶糜烂性毒剂催化降解洗消晶体结构催化水解机制
Keywords:
haloalkane dehalogenases blister agents catalytic hydrolysis decontamination crystal structure catalytic hydrolysis mechanism
分类号:
X172 : Q55
DOI:
10.3724/SP.J.1145.2015.04028
文献标志码:
A
摘要:
芥子气(2,2’-二氯乙基硫醚,HD)是一种糜烂性毒剂,具强烈的细胞毒作用,能使皮肤和各种组织起泡、糜烂和坏死. 近年来,发现多种微生物酶具催化降解HD功能,其中,卤代烷烃脱卤酶(Haloalkane dehalogenases,HLDs)(EC3.8.1.5)能够降解HD产生无毒的硫二甘醇,且催化水解的效果最好,成为环境友好地消除HD关注的焦点. 本文重点综述具较高水解HD活性HLDs,包括LinB、DhaA和DmbA等的发现与进化关系、立体结构、底物特异性和催化水解特征的最新研究成果,以及这些酶在降解HD领域当前和未来潜在的应用. 分析表明这些HLDs虽然归属于同一进化亚家族,但具不同的底物特异性;而这些HLDs一致的催化三元体空间结构决定了它们分享类似的SN2亲核取代催化水解HD的反应机制. 此外,针对现有HLDs在应用方面存在的如催化水解效率有待提高、稳定性差等诸多问题,提出通过分子生物学、基因工程和固相化修饰等技术加以解决.
Abstract:
Sulfur Mustard (bis-(2-chloroethyl) sulphide, HD), also known as yperite, is one of the most important blister agents. It could react with a large number of biological molecules with a strong cytotoxicity effect, resulting in blistering, erosion and necrosis of the skin and various tissues. Recently, several classes of microbial enzymes have been found to be able to degrade HD with high catalytic activity but no disadvantages of the common chemical decontamination of HD. Haloalkane dehalogenases (HLDs, EC3.8.1.5) draws great research attention for environmentally friendly decontaminating HD with only nontoxic thiodiglycol produced. In order to provide theoretical reference basis for enzymatic decontamination of HD, this paper reviews the observation and evolutionary relationship, structures, substrate specificities, catalytic properties and potential applications of these HLDs with high catalytic hydrolysis of HD. The analysis shows that these HLDs belonging to the same subfamily have different substrate specificities but similar spatial structures of the catalytic triad contributing to the common SN2 nucleophilic substitution reaction mechanism for catalytic hydrolysis of HD. The paper also suggests that the problems on improving poor stability of HLDs and efficiency of catalytic hydrolysis of HD should be addressed by methods of molecular biology, genetic engineering and immobilized techniques.

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备注/Memo

备注/Memo:
国民核生化灾害防护国家重点实验室基础研究项目(SKLNBC- 2013-06)资助 Supported by the State Key Laboratory of NBC Protection for Civilian (SKLNBC2013-06)
更新日期/Last Update: 2015-10-29