|本期目录/Table of Contents|

[1]阙万才,黄宁,刘峰,等.甘蔗真核生物翻译起始因子5A基因的克隆与表达分析[J].应用与环境生物学报,2015,21(06):1120-1127.[doi:10.3724/SP.J.1145.2015.04008]
　QUE Wancai,HUANG Ning,LIU Feng,et al.Isolation and expression of a eukaryotic translation initiation factor 5A gene from sugarcane[J].Chinese Journal of Applied & Environmental Biology,2015,21(06):1120-1127.[doi:10.3724/SP.J.1145.2015.04008]
点击复制

甘蔗真核生物翻译起始因子5A基因的克隆与表达分析()
分享到：

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年06期
页码:
1120-1127
栏目:
研究论文
出版日期:
2015-12-25

文章信息/Info

Title:
Isolation and expression of a eukaryotic translation initiation factor 5A gene from sugarcane
作者:
阙万才;  黄宁;  刘峰;  肖新换;  凌辉;  张玉叶;  苏炜华;  苏亚春;  吴期滨 阙友雄
1福建医科大学附属协和医院药学部 福州 350001 2福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室/国家甘蔗产业技术研发中心 福州 350002
Author(s):
QUE Wancai;  HUANG Ning;  LIU Feng;  XIAO Xinhuan;  LING Hui;  ZHANG Yuye;  SU Weihua;  SU Yachun;  WU Qibin;  QUE Youxiong
1Department of Pharmacy, Fujian Medical University Union Hospital, Fuzhou 350001, China 2Key Laboratory of Sugarcane Biology and Genetic Breeding (Fujian), Ministry of Agriculture, Fujian Agriculture and Forestry University/Sugarcane Research & Development Center, China Agricultural Technology System, Fuzhou 350002, China
关键词:
甘蔗; 真核生物翻译起始因子5A; 甘蔗黑穗病菌; 生物信息学; 实时荧光定量PCR
Keywords:
sugarcane;  eIF5A;  Sporisorium scitamineum;  bioinformatics;  Real-time Quantitative PCR
分类号:
Q78 : S566.103.4
DOI:
10.3724/SP.J.1145.2015.04008
文献标志码:
A
摘要:
真核生物翻译起始因子5A（Eukaryotic translation initiation factor 5A，eIF5A）是一种在动植物和真菌体内普遍存在的蛋白质. 为了解eIF5A基因在甘蔗应答生物逆境胁迫中的作用，本研究首先从黑穗病菌胁迫下甘蔗抑制消减杂交（Suppression subtractive hybridization，SSH）文库中获得一条与玉米eIF5A基因（GenBank Accession Number：EU958725.1）同源性为93%的甘蔗EST序列；其次，以此序列作为探针，通过电子克隆技术获得一条甘蔗eIF5A基因的cDNA拼接序列；最后，经RT-PCR扩增和测序验证，结果显示电子克隆序列与测序序列一致，将该序列命名为SceIF5A（GenBank Accession Number：KJ577595）. 生物信息学分析显示，SceIF5A基因cDNA全长1 174 bp，含有长度为483 bp、编码160个氨基酸的完整开放读码框；SceIF5A蛋白是酸性稳定蛋白，分子量（Mr）为17 453.6，含有12个保守氨基酸序列，推测定位于细胞质. 实时荧光定量PCR（Real-time quantitative PCR，RT-qPCR）分析结果表明，在黑穗病菌、水杨酸、茉莉酸甲酯、脱落酸胁迫下，SceIF5A均上调表达，推测SceIF5A基因在甘蔗响应黑穗病菌侵染过程中被诱导表达，且其表达受内源激素信号通路的调控. 本研究获得的SceIF5A基因的结构特征和功能作用模式为研究该基因在甘蔗与黑穗病菌互作中的作用积累了基础数据.
Abstract:
Eukaryotic translation initiation factor 5A (eIF5A) is ubiquitous in animals, plants and fungi. To reveal the responses of sugarcane eIF5A gene to biotic stresses, the present study first obtained an EST sequence with 93% homologs to Zea mays eIF5A (GenBank Accession Number：EU958725.1) from a suppression subtractive hybridization (SSH) library of sugarcane challenged by Sporisorium scitamineum. The EST sequence was then used as the probe for in silico cloning to obtain a putative cDNA sequence of sugarcane eIF5A gene. This gene was validated by reverse transcription-PCR (RT-PCR) amplification sequencing, which showed that the sequence cloned by in silico cloning was in accordance with the sequence amplified by RT-PCR method, and was named as SceIF5A (GenBank Accession Number: KJ577595). Bioinformatics analysis indicated that SceIF5A, with a length of 1 174 bp, containing a 483 bp open reading frame (ORF) encoding 160 amino acids protein, was a stable acidic cytoplasm protein with a weight of 17 453.6 Da containing 12 highly conserved amino acid sequences. Real-time quantitative PCR analysis showed that the expression of SceIF5A was up-regulated under smut fungus infection and the treatments of SA, MeJA and ABA. The results suggested that SceIF5A in sugarcane is most probably involved in response to sugarcane smut fungus infection and to hormone related bioprocess signaling. The results of this study regarding the structure and function of SceIF5A should lay the foundation for further research in the elaboration of functions of this gene during the interaction between sugarcane and S. scitamineum.

参考文献/References:

1 Cheavegatti-Gianotto A, de Abreu HMC, Arruda P, Bespalhok Filho JC, Burnquist WL, Creste S, Di Ciero L, Ferro JA, de Oliveira Figueira AV, de Sousa Filgueiras T. Sugarcane (Saccharum X officinarum): a reference study for the regulation of genetically modified cultivars in Brazil [J]. Trop Plant Biol, 2011, 4 (1): 62-89
2 Que YX, Xu LP. Wu QB, Liu YF, Ling H, Liu YH, Zhang YY, Guo JL, Su YC, Chen JB, Wang SS, Zhang CG. Genome sequencing of Sporisorium scitamineum provides insights into the pathogenic mechanisms of sugarcane smut [J]. BMC Genomics, 2014, 15: 996, doi: 10.1186/1471-2164-15-996
3 许莉萍, 陈如凯. 与甘蔗抗黑穗病基因连锁的RAPD标记筛选[J]. 应用与环境生物学报, 2004, 10 (3): 263-267 [Xu LP, Chen RK. Identification of RAPD marker linked to smut resistance gene in sugarcane [J]. Chin J Appl Environ Biol, 2004, 10 (3): 263-267]
4 莫凤连, 杨丽涛, 潘如科, 宋修鹏, 李杨瑞. 甘蔗黑穗病菌胁迫对甘蔗内源激素含量的影响[J]. 南方农业学报, 2012, 43 (11): 1676-1681 [Mo FL, Yang LT, Pan RK, Song XP, Li YR. Changes of endogenous hormone content in sugarcane under smut pathogen stress [J]. J S Agric, 2012, 43 (11): 1676-1681]
5 Que YX, Su YC, Guo JL, Wu QB, Xu LP. A global view of transcriptome dynamics during Sporisorium scitamineum challenge in sugarcane by RNA-Seq [J]. PLoS ONE, 2014, 9 (8): e106476
6 Que YX, Xu LP, Lin JW, Ruan MH, Zhang MQ, Chen RK. Differential protein expression in sugarcane during sugarcane-Sporisorium scitamineum interaction revealed by 2-DE and MALDI-TOF-TOF/MS [J]. Comp Funct Genom, 2011, 2011, doi: 10.1155/2011/989016
7 Kemper W, Berry K, Merrick W. Purification and properties of rabbit reticulocyte protein synthesis initiation factors M2Balpha and M2Bbeta [J]. J Biol Chem, 1976, 251 (18): 5551-5557
8 Bartig D, Lemkemeier K, Frank J, Lottspeich F, Klink F. The archaebacterial hypusine-containing protein [J]. Eur J Biochem, 1992, 204 (2): 751-758
9 Kyrpides NC, Woese CR. Universally conserved translation initiation factors [J]. Proc Natl Acad Sci, 1998, 95 (1): 224-228
10 Zanelli C, Valentini S. Is there a role for eIF5A in translation? [J]. Amino Acids, 2007, 33 (2): 351-358
11 Parkash J, Vaidya T, Kirti S, Dutt S. Translation initiation factor 5A in Picrorhiza is up-regulated during leaf senescence and in response to abscisic acid [J]. Gene, 2014, 542 (1): 1-7
12 张利姣, 张杰伟, 陈亚娟, 管阳, 王宏芝, 魏建华. 毛白杨真核细胞翻译起始因子5A基因 (PtoeIF5A4) 的克隆与表达分析[J]. 农业生物技术学报, 2013, 21 (8): 949-956 [Zhang LJ, Zhang JW, Chen YJ, Guan Y, Wang HZ, Wei JH. Cloning and expression analysis of the eukaryotic translation initiation factor 5A gene (PtoeIF5A4) in Populus tomentosa [J]. J Agric Biotechnol, 2013, 21 (8): 949-956]
13 Hopkins MT, Lampi Y, Wang TW, Liu Z, Thompson JE. Eukaryotic translation initiation factor 5A is involved in pathogen-induced cell death and development of disease symptoms in Arabidopsis thaliana [J]. Plant Physiol, 2008, 148 (1): 479-489
14 Lan P, Schmidt W. The enigma of eIF5A in the iron deficiency response of Arabidopsis [J]. Plant Signal Behav, 2011, 6 (4): 528-530
15 Huang XL, Liu LX, Chen J, Zhai YH. Comparative proteomic analysis of the response in resistant and susceptible maize inbred lines to infection by Curvularia lunata [J]. Prog Nat Sci, 2009, 19 (7): 845-850
16 Xu J, Zhang B, Jiang C, Ming F. RceIF5A, encoding an eukaryotic translation initiation factor 5A in Rosa chinensis, can enhance thermotolerance, oxidative and osmotic stress resistance of Arabidopsis thaliana [J]. Plant Mol Biol, 2011, 75 (1-2): 167-178
17 Chamot D, Kuhlemeier C. Differential expression of genes encoding the hypusine-containing translation initiation factor, elF-5A, in tobacco [J]. Nucleic Acids Res, 1992, 20 (4): 665-669
18 Chou WC, Huang YW, Tsay WS, Chiang TY, Huang DD, Huang HJ. Expression of genes encoding the rice translation initiation factor, eIF5A, is involved in developmental and environmental responses [J]. Physiol Plant, 2004, 121 (1): 50-57
19 Wang TW, Lu L, Zhang CG, Taylor C, Thompson JE. Pleiotropic effects of suppressing deoxyhypusine synthase expression in Arabidopsis thaliana [J]. Plant Mol Biol, 2003, 52 (6): 1223-1235
20 黄宁, 张玉叶, 凌辉, 罗俊, 吴期滨, 阙友雄. 甘蔗二氨基庚二酸异构酶基因的克隆与表达分析[J]. 热带作物学报, 2013, 34 (11): 2200-2208 [Huang N, Zhang YY, Ling H, Luo J, Wu QB, Que YX. Cloning and expression analysis of a diaminopimelate epimerase gene in sugarcane [J]. Chin J Trop Crops, 2013, 34 (11): 2200-2208]
21 Su YC, Xu LP, Xue BT, Wu QB, Guo JL, Wu LG, Que YX. Molecular cloning and characterization of two pathogenesis-related beta-1,3-glucanase genes ScGluA1 and ScGluD1 from sugarcane infected by Sporisorium scitamineum [J]. Plant Cell Rep, 2013, 32 (10): 1503-1519
22 Gasteiger E, Hoogland C, Gattiker A, Wilkins MR, Appel RD, Bairoch A. Protein Identification and Analysis Tools on the ExPASy Server [M]. New York: Humana Press, 2005
23 Nakai K, Horton P. PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization [J]. Trends Biochem Sci, 1999, 24 (1): 34-35
24 Hua S, Sun Z. Support vector machine approach for protein subcellular localization prediction [J]. Bioinformatics, 2001, 17(8): 721-728
25 Letunic I, Doerks T, Bork P. SMART: recent updates, new developments and status in 2015 [J]. Nucleic Acids Res, 2015, 43 (D1): D257-D260
26 Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer ELL, Tate J, Punta M. Pfam: the protein families database [J]. Nucleic Acids Res, 2013, 42 (D1): D222-230
27 Biasini M, Bienert S, Waterhouse A, Arnold K, Studer G, Schmidt T, Kiefer F, Cassarino TG, Bertoni M, Bordoli L, Schwede T. SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information [J]. Nucleic Acids Res, 2014, 42 (W1): W252-W258
28 阙友雄, 许莉萍, 徐景升, 张积森, 张木清, 陈如凯. 甘蔗基因表达定量 PCR 分析中内参基因的选择[J]. 热带作物学报, 2009, 30 (3): 274-278 [Que YX, Xu LP, Xu JS, Zhang JS, Zhang MQ, Chen RK. Selection of control genes in real-time qPCR analysis of gene expression in sugarcane [J].Chin J Trop Crops, 2009, 30 (3): 274-278]
29 Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2? ΔΔCTmethod [J]. Methods, 2001, 25 (4): 402-408
30 肖新换, 黄宁, 张玉叶, 杨宗锋, 凌辉, 黄珑, 苏炜华, 阙友雄. 甘蔗光合系统Ⅰ亚基O基因的克隆与表达分析[J]. 应用与环境生物学报, 2015, 21 (2): 208-214 [Xiao XH, Huang N, Zhang YY, Yang ZF, Ling H, Huang L, Su WH, Que YX. Cloning and expression of photosystem I subunit O gene from sugarcane [J]. Chin J Appl Environ Biol, 2015, 21 (2): 208-214]
31 肖新换, 黄珑, 黄宁, 张玉叶, 凌辉, 刘峰, 苏炜华, 阙友雄. 甘蔗ScBAK基因及其可变剪接体的克隆与表达分析[J]. 应用与环境生物学报, 2015, 21 (5): 872-881 [Xiao XH, Huang L, Huang N, Zhang YY, Ling H, Liu F, Su WH, Que YX. Cloning and expression analysis of ScBAK gene and its alternative spliceosome gene in sugarcane [J]. Chin J Appl Environ Biol, 2015, 21 (5): 872-881
32 Li YR, Yang LT. Sugarcane agriculture and sugar industry in China [J]. Sugar Tech, 2015, 17(1): 1-8
33 LaO M, Arencibia AD, Carmona ER, Acevedo R, Rodríguez E, León O, Santana I. Differential expression analysis by cDNA-AFLP of Saccharum spp. after inoculation with the host pathogen Sporisorium scitamineum [J]. Plant Cell Rep, 2008, 27 (6): 1103-1111
34 Que YX, Lin JW, Song XX, Xu LP, Chen RK. Differential gene expression in sugarcane in response to challenge by fungal pathogen Ustilago scitaminea revealed by cDNA-AFLP [J]. BioMed Res Int, 2011, 2011, doi: 10.1155/2011/160934
35 Huang N, Zhang YY, Xiao XH, Huang L, Wu QB, Que YX, Xu LP. Identification of smut-responsive genes in sugarcane using cDNA-SRAP [J]. Genet Mol Res, 2015, 14 (2): 6808-6818
36 Que YX, Yandg ZX, Xu LP, Chen RK. Isolation and identification of differentially expressed genes in sugarcane infected by Ustilago scitaminea [J]. Acta Agron Sin, 2009, 35 (3): 452-458
37 Wu QB, Xu LP, Guo JL, Su YC, Que YX. Transcriptome profile analysis of sugarcane responses to Sporisorium scitaminea infection using Solexa sequencing technology [J]. BioMed Res Int, 2013, 2013, http://dx.doi.org/10.1155/2013/298920
38 黄宁. 黑穗病菌胁迫下甘蔗SSH文库构建及差异表达基因的克隆与分析[D]. 福建: 福建农林大学, 2014 [Huang N. Construction of suppressionsubtractive hybridization libraries of sugarcane challenged by Sporisorium scitamineum and cloning/ analysis of several differentially expressed genes [D]. Fujian: Fujian Agriculture and Forestry University, 2014]
39 Shi XP, Yin KC, Zimolo ZA, Stern AM, Waxman L. The subcellular distribution of eukaryotic translation initiation factor, eIF-5A, in cultured cells [J]. Exp Cell Res, 1996, 225 (2): 348-356
40 Ruhl M, Himmelspach M, Bahr GM, Hammerschmid F, Jaksche H, Wolff B, Aschauer H, Farrington GK, Probst H, Bevec D. Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans-activation [J]. J Cell Biol, 1993, 123 (6): 1309-1320
41 Jao DL, Chen KY. Subcellular localization of the hypusine-containing eukaryotic initiation factor 5A by immunofluorescent staining and green fluorescent protein tagging [J]. J Cell Biochem, 2002, 86 (3): 590-600
42 Peat TS, Newman J, Waldo GS, Berendzen J, Terwilliger TC. Structure of translation initiation factor 5A from Pyrobaculum aerophilum at 1.75 ? resolution [J]. Structure, 1998, 6 (9): 1207-1214
43 Kim KK, Hung LW, Yokota H, Kim R, Kim SH. Crystal structures of eukaryotic translation initiation factor 5A from Methanococcus jannaschii at 1.8 ? resolution [J]. PNatl Acad Sci USA, 1998, 95 (18): 10419-10424
44 Yao M, Ohsawa A, Kikukawa S, Tanaka I, Kimura M. Crystal structure of hyperthermophilic archaeal initiation factor 5A: a homologue of eukaryotic initiation factor 5A (eIF-5A) [J]. J Biochem, 2003, 133 (1): 75-81
45 Kunkel BN, Brooks DM. Cross talk between signaling pathways in pathogen defense [J]. Curr Opin Plant Biol, 2002, 5 (4): 325-331
46 阮期平, 周立, 刘勇. 小麦在与水杨酸诱导的应答过程中 PGIP 的积累[J]. 应用与环境生物学报, 2000, 6 (4): 313-316 [Ruan QP, Zhou L, Liu Y. PGIP accumulation in wheat in response to induction with salicylic acid [J]. Chin J Appl Environ Biol, 2000, 6 (4): 313-316]
47 宾金华, 潘瑞炽. 茉莉酸甲酯诱导烟草幼苗抗病与过氧化物酶活性和木质素含量的关系[J]. 应用与环境生物学报, 1999, 5 (2): 160-164 [Bin JH, Pan RC. The relationship of the disease resistance of tobacco seedlings induced by methyl jasmonate with peroxidase activity and lignin content [J]. Chin J Appl Environ Biol, 1999, 5 (2): 160-164]
48 Asselbergh B, De Vleesschauwer D, H?fte M. Global switches and fine-tuning-ABA modulates plant pathogen defense [J]. Mol Plant Microbe In, 2008, 21 (6): 709-719
49 Fontaniella B, Montani A, Rodrlla B, auwPi, Ro D, Solasd MT, Vicentea C, Legaz ME. A role for sugarcane glycoproteins in the resistance of sugarcane to Ustilago scitaminea [J]. Plant Physiol Biochem, 2002, 40 (10): 881-889

相似文献/References:

[1]罗俊,袁照年,张华,等.宿根甘蔗产量性状的稳定性分析[J].应用与环境生物学报,2009,15(04):488.[doi:10.3724/SP.J.1145.2009.00488]
　LUO Jun,YUAN Zhaonian,ZHANG Hua,et al.Stability Analysis on Yield Characters of Sugarcane Ratoon[J].Chinese Journal of Applied & Environmental Biology,2009,15(06):488.[doi:10.3724/SP.J.1145.2009.00488]
[2]叶冰莹,邱思,周平,等.甘蔗蔗糖磷酸合成酶SPSⅡ cDNA片段克隆与表达分析[J].应用与环境生物学报,2011,17(05):673.[doi:10.3724/SP.J.1145.2011.00673]
　YE Bingying,QIU Si,ZHOU Ping,et al.Cloning and Expression Analysis of Sucrose-phosphate Synthase II Gene from Sugarcane[J].Chinese Journal of Applied & Environmental Biology,2011,17(06):673.[doi:10.3724/SP.J.1145.2011.00673]
[3]黄祖新,黄镇,叶冰莹,等.宿根甘蔗根际土壤细菌多样性分析中培养法与非培养法比较研究[J].应用与环境生物学报,2011,17(05):742.[doi:10.3724/SP.J.1145.2011.00742]
　HUANG Zuxin,HUANG Zhen,YE Bingying,et al.Comparison of Culture-dependent and -independent Approaches for Diversity Analysis of Soil Bacteria in the Rhizosphere of Sugarcane[J].Chinese Journal of Applied & Environmental Biology,2011,17(06):742.[doi:10.3724/SP.J.1145.2011.00742]
[4]罗俊,邓祖湖,阙友雄,等.国家甘蔗第七轮区域试验品种的丰产性及稳定性[J].应用与环境生物学报,2012,18(05):734.[doi:10.3724/SP.J.1145.2012.00734]
　LUO Jun,DENG Zuhu,QUE Youxiong,et al.Productivity and Stability of Sugarcane Varieties in the 7th Round National Regional Trial of China[J].Chinese Journal of Applied & Environmental Biology,2012,18(06):734.[doi:10.3724/SP.J.1145.2012.00734]
[5]苏亚春,凌辉,王恒波,等.甘蔗SCoT-PCR反应体系优化与多态性引物筛选及应用[J].应用与环境生物学报,2012,18(05):810.[doi:10.3724/SP.J.1145.2012.00810]
　SU Yachun,LIN Hui,WANG Hengbo,et al.Optimization of SCoT-PCR Reaction System, and Screening and Utilization of Polymorphic Primers in Sugarcane[J].Chinese Journal of Applied & Environmental Biology,2012,18(06):810.[doi:10.3724/SP.J.1145.2012.00810]
[6]肖新换,黄宁,张玉叶,等.甘蔗光合系统Ⅰ亚基O基因的克隆与表达分析[J].应用与环境生物学报,2015,21(02):208.[doi:10.3724/SP.J.1145.2014.09033]
　XIAO Xinhuan,HUANG Ning,ZHANG Yuye,et al.Cloning and expression of photosystem I subunit O gene from sugarcane[J].Chinese Journal of Applied & Environmental Biology,2015,21(06):208.[doi:10.3724/SP.J.1145.2014.09033]
[7]肖新换,黄珑,黄宁,等.甘蔗ScBAK1基因及其可变剪接体的克隆与表达分析[J].应用与环境生物学报,2015,21(05):872.[doi:10.3724/SP.J.1145.2015.03005]
　XIAO Xinhuan,HUANG Long,HUANG Ning,et al.Cloning and expression analysis of ScBAK1 gene and its alternative spliceosome in sugarcane[J].Chinese Journal of Applied & Environmental Biology,2015,21(06):872.[doi:10.3724/SP.J.1145.2015.03005]
[8]苏炜华# 黄 珑# 黄 宁 刘 峰 苏亚春 肖新换 凌 辉 阙友雄.甘蔗细胞色素P450还原酶基因的RT-PCR扩增与表达分析[J].应用与环境生物学报,2016,22(02):173.[doi:10.3724/SP.J.1145.2015.07029]
　SU Weihua#,HUANG Long#,HUANG Ning,et al.RT-PCR amplification and expression analysis of a cytochrome P450 reductasegene from sugarcane[J].Chinese Journal of Applied & Environmental Biology,2016,22(06):173.[doi:10.3724/SP.J.1145.2015.07029]
[9]苏炜华,黄宁,凌辉,等.甘蔗乙醇脱氢酶基因的克隆与表达分析[J].应用与环境生物学报,2017,23(03):474.[doi:2016.0704]
　SU Weihua,HUANG Ning,LING Hui,et al.Cloning and expression of ScADH from sugarcane[J].Chinese Journal of Applied & Environmental Biology,2017,23(06):474.[doi:2016.0704]
[10]黄宁,李聪娜,汤翰臣,等.甘蔗泛素结合酶基因的克隆与表达[J].应用与环境生物学报,2018,24(04):845.[doi:10.19675/j.cnki.1006-687x.2017.11006]
　HUANG Ning,LI Congna,TANG Hanchen,et al.Cloning and expression analysis of a ubiquitin-conjugating enzyme gene in sugarcane[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):845.[doi:10.19675/j.cnki.1006-687x.2017.11006]

备注/Memo

备注/Memo:
国家自然科学基金项目（31101196）、福建省杰出青年科学基金项目（2015J06006）、福建省高等学校新世纪优秀人才支持计划（JA14095）和农业部甘蔗生物学与遗传育种重点实验室开放课题项目资助 Supported by the National Natural Science Foundation of China (31101196), the Natural Science Foundation of Fujian Province (2015J06qyx), the Program for New Century Excellent Talents in Fujian Province Universities (JA14095), and the Open Fund of Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture
更新日期/Last Update: 2016-01-05