|本期目录/Table of Contents|

[1]李端行,王经伟,沈文丽,等.单壁碳纳米管对Arthrobacter sp. W1生长及苯酚降解功能的影响[J].应用与环境生物学报,2015,21(03):483-488.[doi:10.3724/SP.J.1145.2014.11039]
 LI Duanxing,WANG Jingwei,SHEN Wenli,et al.Influence of single-walled carbon nanotubes on the growth and phenol biodegradation characteristics of Arthrobacter sp. W1[J].Chinese Journal of Applied & Environmental Biology,2015,21(03):483-488.[doi:10.3724/SP.J.1145.2014.11039]
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单壁碳纳米管对Arthrobacter sp. W1生长及苯酚降解功能的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年03期
页码:
483-488
栏目:
研究论文
出版日期:
2015-06-25

文章信息/Info

Title:
Influence of single-walled carbon nanotubes on the growth and phenol biodegradation characteristics of Arthrobacter sp. W1
作者:
李端行王经伟沈文丽张照婧厉舒祯李会杰刘紫嫣马桥曲媛媛周集体
大连理工大学环境学院,工业生态与环境工程教育部重点实验室 大连 116024
Author(s):
LI Duanxing WANG Jingwei SHEN Wenli ZHANG Zhaojing LI Shuzhen LI Huijie LIU Ziyan MA Qiao QU Yuanyuan ZHOU Jiti
Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China
关键词:
单壁碳纳米管苯酚作用机制生物降解Arthrobacter sp.
Keywords:
single-walled carbon nanotubes phenol action mechanisms biodegradation Arthrobacter sp.
分类号:
X172
DOI:
10.3724/SP.J.1145.2014.11039
文献标志码:
A
摘要:
单壁碳纳米管(SWCNTs)对大肠杆菌等模式菌株的生长抑制作用明显,为了解SWCNTs对具有环境功能的菌株的作用,以苯酚降解菌Arthrobacter sp. W1为对象,考察不同浓度SWCNTs下菌株W1的生长曲线和苯酚降解曲线,并通过扫描电镜观察、细胞凋亡检测、DNA泄漏量分析和活性氧产量分析考察作用机制. 结果表明,特定浓度范围的SWCNTs(0.5-5.0 mg/L)会加快W1的苯酚降解速率,且1.5-2.0 mg/L SWCNTs不抑制W1的生长. SWCNTs在溶液中形成团簇并吸附W1细胞,且对W1产生以物理穿刺为主的毒性作用,但在特定浓度范围的SWCNTs条件下,SWCNTs-菌株-苯酚体系增加了菌体与苯酚的接触机会,从而对W1生长和苯酚降解产生明显的促进作用. 本研究结果可为进一步揭示SWCNTs的环境微生物效应提供理论依据.
Abstract:
With the extensive production and application of single-walled carbon nanotubes (SWCNTs), their potential effects on biological systems and human health have attracted much research attention. Most researches used Escherichia coli as model strain and observed strong antimicrobial activity of SWCNTs, rarely paying attention to the effects of SWCNTs on functional microorganisms. This research investigated the growth curves and degradation curves of a phenol-degrading bacterium Arthrobacter sp. W1 influenced by different concentrations of SWCNTs, through scanning electron microscopic observation, viability test, cellular integrity analysis and reactive oxidative stress analysis. The results showed that 0.5-5.0 mg/L SWCNTs would accelerate phenol degrading process, and 1.5-2.0 mg/L SWCNTs had no antibacterial effects on strain W1. SWCNTs aggregates would adsorb cells with the toxicity mainly caused by physical piercing. While in systems with certain concentrations of SWCNTs, the SWCNTs-cells-phenol coexisting systems would create a suitable microenvironment for W1 growth and phenol degradation. This study would provide theoretical foundation for revealing the microbial effects of SWCNTs.

参考文献/References:

1 Endo M, Takeuchi K, Kobori K, Takahashi K, Kroto HW, Sarkar A. Pyrolytic carbon nanotubes from vapor-grown carbon fibers [J]. Carbon, 1995, 33 (7): 873-881
2 Cai Y, Lin JD, Chen HB, Zhang HB, Lin GD, Liao DW. Novel Ru-K/carbon nanotubes catalyst for ammonia synthesis [J]. Chin Chem Lett, 2000, 11 (4): 373-374
3 Salvetat JP, Briggs GAD, Bonard JM, Bacsa RR, Kulik AJ, Stockli T, Burnham NA, Forro L. Elastic and shear moduli of single-walled carbon nanotube ropes [J]. Phys Rev Lett, 1999, 82 (5): 944
4 Lam CW, James JT, McCluskey R, Hunter RL. Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation [J]. Toxicol Sci, 2004, 77 (1): 126-134
5 Muller J, Huaux F, Moreau N, Misson P, Heilier JF, Delos M, Arras M, Fonseca A, Nagy JB, Lison D. Respiratory toxicity of multi-wall carbon nanotubes [J]. Toxicol Appl Pharm, 2005, 207 (3): 221-231
6 金霏霏, 尹颖, 黄娟, 郭红岩, 杨柳燕. 腐殖酸 (HA) 作用下纳米氧化锌对鲫鱼的毒性效应[J]. 应用与环境生物学报, 2011, 17 (6): 829-832 [Jin FF, Yin Y, Huang J, Guo HY, Yang LY. Effect of HA on ecotoxicity of nanoZnO in Carassius auratus [J]. Chin J Appl Environ Biol, 2011, 17 (6): 829-832]
7 刘银银, 李峰, 孙庆业, 谢永宏. 湿地生态系统土壤微生物研究进展[J]. 应用与环境生物学报, 2013, 19 (3): 547-552 [Liu YY, Li F, Sun QY, Xie YH. Review on the study of soil microorganisms in wetland ecosystems [J]. Chin J Appl Environ Biol, 2013, 19 (3): 547-552]
8 Kang S, Pinault M, Pfefferle LD, Elimelech M. Single-walled carbon nanotubes exhibit strong antimicrobial activity [J]. Langmuir, 2007, 23: 8670-8673
9 Kang S, Herzberg M, Rodrigues DF, Elimelech M. Antibacterial effects of carbon nanotubes: size does matter! [J]. Langmuir, 2008, 24: 6409-6413
10 Sayes CM, Gobin AM, Ausman KD, Mendez J, West JL, Colvin VL. Nano-C60 cytotoxicity is due to lipid peroxidation [J]. Biomaterials, 2005, 26 (36): 7587-7595
11 Ong YT, Ahmad AL, Zein SHS, Tan SH. A review on carbon nanotubes in an environmental protection and green engineering perspective [J]. Braz J Chem Eng, 2010, 27 (2): 227-242
12 Rodrigues DF, Elimelech M. Toxic effects of single-walled carbon nanotubes in the development of E. coli biofilm [J]. Environ Sci Technol, 2010, 44 (12): 4583-4589
13 王平. 高盐含酚废水生物处理及微生物群落结构研究[D]. 大连: 大连理工大学, 2009 [Wang P. Studies on biodegradation of phenolic hyper-saline wastewater and dynamics of microbial community structure during the treatment process [D]. Dalian: Dalian University of Technology, 2009]
14 Yang C, Mamouni J, Tang Y, Yang L. Antimicrobial activity of single-walled carbon nanotubes: length effect [J]. Langmuir, 2010, 26 (20): 16013-16019
15 Bai Y, Park IS, Lee SJ, Bae TS, Watari F, Uo M, Lee MH. Aqueous dispersion of surfactant-modified multiwalled carbon nanotubes and their application as an antibacterial agent [J]. Carbon, 2011, 49 (11): 3663-3671
16 Arias LR, Yang L. Inactivation of bacterial pathogens by carbon nanotubes in suspensions [J]. Langmuir, 2009, 25 (5): 3003-3012
17 Fan JL, Cai HB, Tan WS. Role of the plasma membrane ROS-generating NADPH oxidase in CD34+ progenitor cells preservation by hypoxia [J]. J Bio Technol, 2007, 130 (4): 455-462.
18 孙明礼, 成荣明, 徐学诚, 陈奕卫, 李茂刚. 苯酚及取代酚在碳纳米管上的吸附研究[J]. 化学研究与应用, 2006, 18 (1): 13-18 [Sun ML, Cheng RM, Xu XC, Chen YW, Li MG. Studies on adsorption of phenol and substituted phenols on carbon nanotubes [J]. Chem Res Appl, 2006, 18 (1): 13-18]
19 崔春月, 郑庆柱, 胡春光, 刘强. 多壁碳纳米管对水中五氯苯酚的吸附性能研究[J]. 环境科学与技术, 2010, 33 (5): 67-70 [Cui CY, Zheng QZ, Hu CG, Liu Q. Adsorption of pentachlorophenol on multi-walled carbon nanotubes [J]. Environ Sci Technol, 2010, 33 (5): 67-70]
20 Song MY, Zeng LZ, Yuan SP, Yin JF, Wang HL, Jiang GB. Study of cytotoxic effects of single-walled carbon nanotubes functionalized with different chemical groups on human MCF7 cells [J]. Chemosphere, 2013, 92: 576-582
21 Park HJ, Nguyen TTM, Yoon J, Lee C. Role of reactive oxygen species in Escherichia coli inactivation by cupric ion [J]. Environ Sci Technol, 2012, 46 (20): 11299-11304
22 Cui Y, Zhao YY, Tian Y, Zhang W, Lu XY, Jiang XY. The molecular mechanism of action of bactericidal gold nanoparticles on Escherichia coli [J]. Biomaterials, 2012, 33: 2327-2333

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备注/Memo

备注/Memo:
国家自然科学基金项目(21176040)、新世纪优秀人才支持计划(NCET-13-0077)和中央高校基本科研业务费专项资金(DUT14YQ107)项目资助 Supported by the National Natural Science Foundation of China (21176040), the Program for New Century Excellent Talents in University (NCET-13-0077), and the Fundamental Research Funds for the Central Universities (DUT14YQ107).
更新日期/Last Update: 2015-06-23