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[1]杨希智,王长庭,字洪标,等.三江源区不同建植年限人工草地土壤微生物群落结构特征[J].应用与环境生物学报,2015,21(02):341-349.[doi:10.3724/SP.J.1145.2014.10033]
 YANG Xizhi,WANG Changting,ZI Hongbiao,et al.Soil microbial community structure characteristics in artificial grassland with different cultivation years in the headwater region of Three Rivers, China[J].Chinese Journal of Applied & Environmental Biology,2015,21(02):341-349.[doi:10.3724/SP.J.1145.2014.10033]
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三江源区不同建植年限人工草地土壤微生物群落结构特征()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年02期
页码:
341-349
栏目:
研究论文
出版日期:
2015-04-25

文章信息/Info

Title:
Soil microbial community structure characteristics in artificial grassland with different cultivation years in the headwater region of Three Rivers, China
作者:
杨希智 王长庭 字洪标 刘敏
西南民族大学生命科学与技术学院 成都 610041
Author(s):
YANG Xizhi WANG Changting ZI Hongbiao LIU Min
College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
关键词:
三江源区人工草地PLFA土壤微生物群落土壤养分
Keywords:
headwater region of Three Rivers artificial grassland PLFA soil microbial community soil nutrient
分类号:
S812.2
DOI:
10.3724/SP.J.1145.2014.10033
文献标志码:
A
摘要:
近年来,对退化生态系统恢复的研究逐渐由地上向地下部分转移,地下部分特别是土壤微生物群落对土壤的恢复机理和过程已倍受关注. 运用常规实验室分析法和磷脂脂肪酸法,研究三江源区不同建植年限人工草地土壤养分和微生物群落结构及其分布特征. 结果显示:土壤总磷脂脂肪酸(总PLFAs)、真菌PLFAs(F PLFAs)、细菌PLFAs(B PLFAs)、革兰氏阳性菌PLFAs(G+ PLFAs)、革兰氏阴性菌PLFAs(G- PLFAs)含量与PLFA类型随建植年限的增加呈先降低后升高的变化趋势,随土层深度的增加而降低. 土壤全量、速效养分含量均随建植年限的增加呈现同样的变化规律. 主成分分析表明,在0-10 cm、10-20 cm土层中,4年和16年人工草地土壤微生物群落PLFA类型丰富多样,群落结构更加复杂稳定,而7年和9年的土壤微生物群落结构较为单一. 相关分析表明,总PLFAs、B PLFAs、F PLFAs、G+ PLFAs和G- PLFAs含量与土壤全量和速效养分含量(除速效氮、速效磷)均呈极显著或显著正相关(P < 0.01;P < 0.05). 综上,在合理放牧条件下,人工草地的建植有利于土壤微生物生物量的提高和群落结构的改变. 利用微生物PLFA含量和类型的多样性作为监测土壤环境的指标,能及时有效地判别土壤养分的变化情况,有利于及时管理人工草地.
Abstract:
Recent studies of restoration mechanisms and processes are concentrated more and more on belowground systems, especially the soil microbial community. In this study, soil microbial community structure and distribution characteristics were evaluated in artificial grassland with different cultivation years in the headwater region of Three Rivers, China. Soil nutrients were investigated by conventional laboratory analysis; the soil microbial composition was analyzed by phospholipid fatty acid (PLFA) method. The results showed that the PLFAs decreased with soil depth, with PLFA abundance and contents of total PLFAs, including fungal PLFAs (F PLFAs), bacterial PLFAs (B PLFAs), Gram-positive PLFAs (G+ PLFAs) as well as Gram-negative PLFAs (G- PLFAs) initially decreasing but subsequently increasing with the cultivation year. Contents of soil organic matter (SOM), total nitrogen (TN), total phosphorus (TP), available nitrogen (AN) and available phosphorus (AP) followed the same trend as the contents of PLFAs. Soil microbial communities at 0-20 cm soil were abundant and diverse in 4-year and 16-year artificial grasslands, but less so in 7-year and 9-year cultivations. Correlation analysis showed that the contents of total PLFAs, B PLFAs, F PLFAs G+ PLFAs and G- PLFAs were significantly and positively correlated to the concentrations of SOM, TN, TP, AN and AP (P < 0.01); fungal contents were significantly and positively correlated with TN and TP (P < 0.01) besides with organic matter (P < 0.05), and also positively, though not significantly, related to AN and AP. Therefore, under reasonable grazing conditions, artificial grasslands can be planted to increase soil microbial biomass and change microbial community structure. Soil microbial biomass and types as monitoring indicators of soil environment can help to timely identify changes of soil nutrients, which is beneficial for efficient management of artificial grasslands.

参考文献/References:

1 丁忠兵. 论三江源地区的生态地位与可持续发展[J]. 青海社会科学, 2006 (2): 45-50 [Ding ZB. On the ecological status and sustainable development in the Headwater Region of Three Rivers [J]. Soc Sci Qinghai, 2006 (2): 45-50]
2 冯瑞章, 周万海, 龙瑞军, 马玉寿, 齐文娟. 江河源区不同建植期人工草地土壤养分及微生物量磷和磷酸酶活性研究[J]. 草业学报, 2007, 16 (6): 1-6 [Feng RZ, Zhou WH, Long RJ, Ma YS, Qi WJ. Study on the changes of soil nutrient, microbial biomass P and neutral phosphatase activity of artificial grassland sown in different years in the headwaters of the Yangtze and Yellow Rivers [J]. Acta Pratac Sin, 2007, 16 (6): 1-6]
3 曹广民, 龙瑞军. 三江源区“黑土滩”型退化草地自然恢复的瓶颈及解决途径[J]. 草地学报, 2009, 17 (1): 4-9 [Cao GM, Long RJ. The bottleneck and its resolutions to the natural recovery of black soil type degraded grassland in the three river source [J]. Acta Agrestia Sin, 2009, 17 (1): 4-9]
4 王斌, 李洁, 姜微微, 赵亮, 古松. 草地退化对三江源区高寒草甸生态系统CO2能量的影响及其原因[J]. 中国环境科学, 2012, 32 (10): 1764-1771 [Wang B, Li J, Jiang WW, Zhao L, Gu S. Impacts of the rangeland degradation on CO2 flux and the underlying mechanisms in the Three-River Source Region on the Qinghai-Tibetan Plateau [J]. China Environ Sci, 2012, 32 (10): 1764-1771]
5 温璐, 董世魁, 朱磊, 施建军, 刘德梅, 王彦龙, 马玉寿. 环境因子和干扰强度对高寒草甸植物多样性空间分异的影响[J]. 生态学报, 2011, 31 (7): 1844-1854 [Wen L, Dong SK, Zhu L, Shi JJ, Liu DM, Wang YL, Ma YS. The effect of natural factors and disturbance intensity on spacial heterogeneity of plant diversity in alpine meadow [J]. Acta Ecol Sci, 2011, 31 (7): 1844-1854]
6 王长庭, 龙瑞军, 王启兰, 景增春, 施建军, 杜岩功, 曹广民. 三江源区高寒草甸不同退化演替阶段土壤有机碳和微生物量碳的变化[J]. 应用与环境生物学报, 2008, 14 (2): 225-230 [Wang CT, Long RJ, Wang QL, Jing ZC, Shi JJ, Du YG, Cao GM. Changes in soil organic carbon and microbial biomass carbon at different degradation successional stages of alpine meadows in the Headwater Region of Three Rivers in China[J]. Chin J Appl Environ Biol, 2008, 14 (2): 225-230]
7 刘纪远, 徐新良, 邵全琴. 近30年来青海三江源地区草地退化的时空特征[J]. 地理学报, 2008, 63 (4): 364-376 [Liu JY, Xu XL, Shao QQ. The spatial and temporal characteristics of grassland degradation in the Three-River Headwaters Region in Qinghai province [J]. Acta Geogr Sin, 2008, 63 (4): 364-376]
8 赵新全, 周华坤. 三江源区生态环境退化, 恢复治理及其可持续发展[J]. 中国科学院院刊, 2006, 20 (6): 471-476 [Zhao XQ, Zhou HK. Eco-environmental degradation vegetation regeneration and sustainable development in the head-waters of three rivers on Tibetan plateau [J]. China Acad J Electr Publ House, 2006, 20 (6): 471-476]
9 王学霞, 董世魁, 李媛媛, 李小艳, 温璐, 吴娱. 三江源区草地退化与人工恢复对土壤理化性状的影响[J]. 水土保持学报, 2012, 26 (4): 113-117 [Wang XX, Dong SK, Li YY, Li XY, Wen L, Wu Y. Effects of grassland degradation and artificial restoration on soil physicochemical properties in three-river headwater [J]. J Soil Water Conserv, 2012, 26 (4): 113-117]
10 董全民, 赵新全, 马玉寿, 代勇, 王启基, 施建军, 王柳英. 放牧强度对江河源区垂穗披碱草(Elymus natans)/星星草(Puccinellia tenuflora)混播草地群落和高原鼠兔 (Ochotona curzoniae)的影响[J]. 西北农业学报, 2006, 15 (2): 28-33 [Dong QM, Zhao XQ, Ma YS, Dai Y, Wang QL, Shi JJ, Wang LY. Effects of grazing intensity on mixed-sown community of Elymus natans and Puccinellia tenuflora and Ochotona curzoniae in Yangtze and Yellow River Headwater Region [J]. Acta Agric Bor-occid Sin, 2006, 15 (2): 28-33]
11 王长庭, 曹广民, 王启兰, 施建军, 杜岩功, 龙瑞军. 三江源地区不同建植期人工草地植被特征及其与土壤特征的关系[J]. 应用生态学报, 2007, 18 (11): 2426-2431 [Wang CT, Cao GM, Wang QL, Shi JJ, Du YG, Long RJ. Characteristics of artificial grassland plant communities with different establishment duration and their relationships with soil properties in the source region of Three Rivers in China [J]. Chin J Appl Ecol, 2007, 18 (11): 2426-2431]
12 温军, 周华坤, 姚步青, 李以康, 赵新全, 陈哲, 连利叶, 郭凯先. 三江源区不同退化程度高寒草原土壤呼吸特征[J]. 植物生态学报, 2014, 38 (2): 209-218 [Wen J, Zhou HK, Yao BQ, Li YK, Zhao XQ, Chen Z, Lian LY, Guo KX. Characteristics of soil respiration in different degraded alpine grassland in the source region of Three-River [J]. Chin J Plant Ecol, 2014, 38 (2): 209-218]
13 刘艳书, 樊江文, 李愈哲, 张良侠. 三江源地区不同剥蚀退化高寒草甸群落生物量与多样性特征[J]. 草业学报, 2014, 23 (3): 1-7 [Liu YS, Fan JW, Li YZ, Zhang LX. Plant community productivity and diversity on alpine meadow steppe in the Three River Headwater Region, Qinghai Province under different denudation levels [J]. Acta Pratac Sin, 2014, 23 (3): 1-7]
14 张莉, 党军, 刘伟, 王启兰, 向泽宇, 王长庭. 高寒草甸连续围封与施肥对土壤微生物群落结构的影响[J]. 应用生态学报, 2012, 23 (11): 3072-3078 [Zhang L, Dang J, Wang QL, Xiang ZY, Wang CT. Effects of continuous enclosure and fertilization on soil microbial community structure in alpine meadow [J]. Chin J Appl Ecol, 2012, 23 (11): 3072-3078]
15 于树, 汪景宽, 李双异. 应用 PLFA 方法分析长期不同施肥处理对玉米地土壤微生物群落结构的影响[J]. 生态学报, 2008, 28 (9): 4221-4227 [Yu S, Wang JK, Li SY. Effect of long-term fertilization on soil microbial community structure in corn field with the method of PLFA [J]. Acta Ecol Sin, 2008, 28 (9): 4221-4227]
16 颜慧, 蔡祖聪, 钟文辉. 磷脂脂肪酸分析方法及其在土壤微生物多样性研究中的应用[J]. 土壤学报, 2006, 43 (5): 851-859 [Yan H, Cai ZC, Zhong WH. PLFA analysis and its applications in the study of soil microbial diversity [J]. Acta Pedol Sin, 2006, 43 (5): 851-859]
17 钟文辉, 蔡祖聪. 土壤微生物多样性研究方法[J]. 应用生态学报, 2004, 15 (5): 899-904 [Zhong WH, Cai ZC. Methods for studying soil microbial diversity [J]. Chin J Appl Ecol, 2004, 15 (5): 899-904]
18 王长庭, 龙瑞军, 王启兰, 刘伟, 景增春, 张莉. 三江源区不同建植年代人工草地群落演替与土壤养分变化[J]. 应用与环境生物学报, 2009, 15 (6): 737-744 [Wang CT, Long RJ, Wang QL, Liu W, Jing ZC, Zhang L. Community succession of differently aged artificial grasslands and their soil nutrient changes in three rivers’ source regions in Qinghai, China [J]. Chin J Appl Environ Biol, 2009, 15 (6): 737-744]
19 鲍士旦. 土壤农化分析[M]. 北京: 中国农业出版社, 2000: 25-82 [Bao SD. Analysis of Agricultural Chemistry [M]. Beijing: China Agriculture Press, 2000: 25-82]
20 Frosteg?rd ?, B??th E, Tunlio A. Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis [J]. Soil Biol Biochem, 1993, 25 (6): 723-730
21 Bligh E, Dyer WJ. A rapid method of total lipid extraction and purification [J]. Canad J Biochem Phys, 1959, 37 (8): 911-917
22 Frosteg?rd?, Tunlid A, B??th, E. Microbial biomass measured as total lipid phosphate in soils of different organic content [J]. J Microb Meth, 1991, 14 (3): 151-163
23 Frosteg?rd ?, B??th E. The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil [J]. Biol Fertil Soils, 1996, 22 (1-2): 59-65
24 文倩, 林启美, 赵小蓉, 李贵桐, 赵沛一. 北方农牧交错带林地、耕地和草地土壤微生物群落结构特征的PLFA分析[J]. 土壤学报, 2008, 45 (2): 321-327 [Wen Q, Lin QM, Zhao XR, Li GT, Zhao PY. Application of PLFA analysis in determination of soil microbial community structure in woodland, cropland and grassland in farmland-pasture interleaving zone of north China [J]. Acta Pedol Sin, 2008, 45 (2): 321-327]
25 Zelles L. Phospholipid fatty acid profiles in selected members of soil microbial communities [J]. Chemosphere, 1997, 35 (1): 275-294
26 Zogg GP, Zak DR, Ringelberg DB, White DC, Macdonald NW, Pregitzer KS. Compositional and functional shifts in microbial communities due to soil warming [J]. Soil Sci Soc Am J, 1997, 61 (2): 475-481
27 Zelles L. Fatty acid patterns of phospholipids and lipopolysaccharides in the characterisation of microbial communities in soil: A review [J]. Biol Fertil Soils, 1999, 29 (2): 111-129
28 Knoblauch C, Zimmermann U, Blumenberg M, Michaelis W, Pfeiffer EM. Methane turnover and temperature response of methane-oxidizing bacteria in permafrost-affected soils of northeast Siberia [J]. Soil Biol Biochem, 2008, 40 (12): 3004-3013
29 Myers RT, Zak DR, White DC, Peacock A. Landscape-level patterns of microbial community composition and substrate use in upland forest ecosystems [J]. Soil Sci Soc Am J, 2001, 65 (2): 359-367
30 Zechmeister-Boltenstern S, Michel K, Pfeffer M. Soil microbial community structure in European forests in relation to forest type and atmospheric nitrogen deposition [J]. Plant Soil, 2011, 343 (1-2): 37-50
31 Yu M, Zeng G, Chen Y, Yu H, Huang D, Tang L. Influence of Phanerochaete chrysosporium on microbial communities and lignocellulose degradation during solid-state fermentation of rice straw [J]. Proc Biochem, 2009, 44 (1): 17-22
32 张焕军, 郁红艳, 丁维新. 长期施用有机无机肥对潮土微生物群落的影响[J]. 生态学报, 2011, 31 (12): 3308-3314 [Zhang HJ, Yu HY, Ding WX. The influence of the long-term application of organic manure and mineral fertilizer on microbial community in calcareous fluvo-aquic soil [J]. Acta Ecol Sin, 2011, 31 (12): 3308-3314]
33 王蓓, 孙庚, 罗鹏, 王志远, 张艳博, 吴宁, 罗光荣. 模拟升温和放牧对高寒草甸土壤微生物群落的影响[J]. 应用与环境生物学报, 2011, 17 (2): 151-157 [Wang B, Sun G, Luo P, Wang ZY, Zhang YB, Wu N, Luo GR. Microbial communities of alpine meadow soil in the eastern Qinghai-Tibetan plateau subjected to experimental warming and grazing [J]. Chin J Appl Environ Biol, 2011, 17 (2): 151-157]
34 Nüsslein K, Tiedje JM. Soil bacterial community shift correlated with change from forest to pasture vegetation in a tropical soil [J]. Appl Environ Microbiol, 1999, 65 (8): 3622-3626
35 章家恩, 刘文高, 胡刚. 不同土地利用方式下土壤微生物数量与土壤肥力的关系[J]. 土壤与环境, 2002, 11 (2): 140-143 [Zhang JE, Liu WG, Hu G. The relationship between quantity index of soil microorganisms and soil fertility of different land use systems [J]. Soil Environ Sci, 2002, 11 (2): 140-143]
36 刘世贵, 葛绍荣, 龙章富. 川西北退化草地土壤微生物数量与区系研究[J]. 草业学报, 1994, 3 (4): 70-76 [Liu SG, Ge SR, Long ZF. Studies on soil microorganism numbers and microbiota of degenerated rangelands in Northwest Region of Sichuan. P. R. China [J]. Acta Pratac Sin, 1994, 3 (4): 70-76]
37 韩玉竹, 陈秀蓉, 王国荣, 杨成德, 徐长林. 东祁连山高寒草地土壤微生物分布特征初探[J]. 草业科学, 2007, 24 (4): 14-18 [Han YZ, Chen XR, Wang GR, Yang CD, Xu CL. Distribution characteristics of soil micro-organism in alpine grassland of Eastern Qilian Mountains [J]. Pratac Sci, 2007, 24 (4): 14-18]
38 胡雷, 王长庭, 王根绪, 马力, 刘伟, 向泽宇. 三江源区不同退化演替阶段高寒草甸土壤酶活性和微生物群落结构的变化[J]. 草业学报, 2014, 23 (3): 8-19 [Hu L, Wang CT, Wang GX, Ma L, Liu W, Xiang ZY. Changes in the activities of soil enzymes and microbial community structure at different degradation successional stages of alpine meadow in the headwater region of Three Rivers, China [J]. Acta Pratac Sin, 2014, 23 (3): 8-19]
39 Byss M, Elhottová D, T?íska J, Baldrian M. Fungal bioremediation of the creosote-contaminated soil: Influence of Pleurotusostreatus and Irpexlacteus on polycyclic aromatic hydrocarbons removal and soil microbial community composition in the laboratory-scale study [J]. Chemosphere, 2008, 73 (9): 1518-1523
40 Huang H, Zhang S, Wu N, Luo L. Influence of Glomusetunicatum/Zea mays mycorrhiza on atrazine degradation, soil phosphatase and dehydrogenase activities, and soil microbial community structure [J]. Soil Biol Biochem, 2009, 41 (4): 726-734
41 时鹏, 王淑平, 贾书刚, 高强, 孙晓强. 三种种植方式对土壤微生物群落组成的影响[J]. 植物生态学报, 2011, 35 (9): 965-972 [Shi P, Wang SP, Jia SG, Gao Q, Sun XQ. Effects of three planting patterns on soil microbial community composition [J]. Chin J Plant Ecol, 2011, 35 (9): 965-972]
42 王启兰, 王长庭, 刘伟, 曹广民, 龙瑞军. 三江源区不同建植年限人工草地植物群落与土壤微生物生理类群的变化[J]. 应用生态学报, 2009, 20 (11): 2646-2651 [Wang QL, Wang CT, Liu W, Cao GM, Long RJ. Changes in plant communities and soil microbial physiological groups of artificial grasslands established for different years in headwater region of Yangtze River and Yellow River [J]. Chin J Appl Ecol, 2009, 20 (11): 2646-2651]
43 蔡晓布, 钱成, 张元, 普琼. 西藏中部地区退化土壤秸秆还田的微生物变化特征及其影响[J]. 应用生态学报, 2004, 15 (3): 463-468 [Cai XB, Qian C, Zhang Y, Pu Q. Microbial characteristics of straw amended degraded soils in central Tibet and its effect on soil fertility [J]. Chin J Appl Ecol, 2004, 15 (3): 463-468]
44 赵帅, 张静妮, 赖欣, 杨殿林, 赵建宁, 李刚, 邹雨坤. 放牧与围栏内蒙古针茅草原土壤微生物生物量碳、氮变化及微生物群落结构PLFA分析[J]. 农业环境科学学报, 2011, 30 (6): 1126-1134 [Zhao S, Zhang JN, Lai X, Yang DL, Zhao JN, Li G, Zou YK. Analysis of microbial biomass C, N and soil microbial community structure of stipe steppes using PLFA at grazing and fenced in Inner Mongolia, China [J]. J Agro-Environ Sci, 2011, 30 (6): 1126-1134]

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备注/Memo

备注/Memo:
国家自然科学基金项目(31370542)、国家重点基础研究发展计划(973计划)(2013CBA01807)、四川省教育厅创新团队项目(14TD0049)和西南民族大学研究生创新型科研项目(CX2014SZ113)资助 Supported by the National Natural Science Foundation of China (31370542), the National Basic Research Program of China (2013CBA01807), the Innovation Team Project of Education Office of Sichuan Province (14TD0049), and the Graduate Innovative Research Project of Southwest University for Nationalities (CX2014SZ113)
更新日期/Last Update: 2015-04-28