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[1]濮生财,鲁璐,焦威,等.不同品种苦荞中的4种活性黄酮含量[J].应用与环境生物学报,2015,21(03):470-476.[doi:10.3724/SP.J.1145.2014.09030]
 PU Shengcai,LU Lu,JIAO Wei,et al.Contents of four active flavonoids in different species of buckwheat[J].Chinese Journal of Applied & Environmental Biology,2015,21(03):470-476.[doi:10.3724/SP.J.1145.2014.09030]
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不同品种苦荞中的4种活性黄酮含量()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年03期
页码:
470-476
栏目:
研究论文
出版日期:
2015-06-25

文章信息/Info

Title:
Contents of four active flavonoids in different species of buckwheat
作者:
濮生财鲁璐焦威邵华武阳小成
1成都理工大学材料与化学化工学院 成都 610059 2中国科学院成都生物研究所 成都 610041
Author(s):
PU Shengcai LU Lu JIAO Wei SHAO Huawu YANG Xiaocheng
1College of Material Chemistry and Chemical Technology, Chengdu University of Technology, Chengdu 610059, China 2Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
关键词:
苦荞芦丁山奈酚-3-芸香糖苷槲皮素山奈酚HPLC
Keywords:
buckwheat rutin kmapferol-3-rutinoside quercetin kaempferol HPLC
分类号:
Q948.744.06
DOI:
10.3724/SP.J.1145.2014.09030
文献标志码:
A
摘要:
利用HPLC测定21个不同品种苦荞籽粉和麸皮中芦丁、山奈酚-3-芸香糖苷、槲皮素和山奈酚的含量. 采用 RP-HPLC,用Damonsil C18色谱柱(4.6 mm × 250 mm,5 μm),以乙腈-0.2%冰醋酸水溶液为流动相进行梯度洗脱,流速为1.0 mL/min,检测波长为354 nm,柱温30 ℃. 结果显示,在室温状态下,21种不同品种的苦荞种子中水分含量在 4.907 3%-6.993 6%之间,恒重状态下,麸皮质量分数为21.84%-30.03%,麸皮重量约占苦荞籽粒质量的1/4. 芦丁、山奈酚-3-芸香糖苷、槲皮素和山奈酚的线性范围分别在20-400 μg/mL、2-40 μg/mL、1-20 μg/mL、0.5-10 μg/mL;不同品种苦荞籽粉中,芦丁、山奈酚-3-芸香糖苷、槲皮素和山奈酚的含量范围分别为12.137 0-20.318 6 mg/g、1.478 7-2.871 9 mg/g、0.014 6- 0.901 5 mg/g、0-0.015 9 mg/g,4种主要黄酮的总含量为14.015 1-22.871 7 mg/g;麸皮中芦丁、山奈酚-3-芸香糖苷、槲皮素和山奈酚的含量范围分别为4.269 0-12.118 9 mg/g、0.258 5-1.014 9 mg/g、0.061 8-0.735 4 mg/g、0-0.026 3 mg/g,4种主要黄酮的总含量为4.651 1-13.148 6 mg/g. 本研究表明,无论在籽粉还是麸皮中,芦丁都是含量最高的黄酮,其次为山奈酚-3-芸香糖苷和槲皮素,山奈酚的含量在万分之一以下;本研究可为苦荞籽粉和麸皮中主要黄酮的开发和优良品种的利用提供重要依据.
Abstract:
In order to examine the merits of buckwheat, four main flavonoids (rutin, kaempferol-3-rutinoside, quercetin and kaempferol) were analyzed by using reversed-phase HPLC in 21 kinds of buckwheat, with chromatography column of Damonsil C18 (4.6 mm × 250 mm, 5 μm), mobile phase of acetonitrile-0.2% acetic acid solution, flow rate of 1.0 mL/min, detection wavelength of 354 nm, and column temperature of 30 oC. The results showed an average moisture content of 4.907 3-6.993 6% in 21 kinds of different species of buckwheat seeds at room temperature; under constant weight state, the bran mass fraction was 21.84-30.03%, accounting for 1/4 of buckwheat seeds quality. The linear ranges of rutin, kaempferol-3-rutinoside, quercetin and kaempferol were 20-400 μg/mL, 2-40 μg/mL, 1-20 μg/mL, 0.5-10 μg/mL, respectively. In the powder of 21 kinds of buckwheat seeds, the content ranges of rutin, kaempferol-3-rutinoside, quercetin and kaempferol were 12.137 0-20.318 6 mg/g, 1.478 7-2.871 9 mg/g, 0.014 6-0.901 5 mg/g, 0-0.015 9 mg/g, respectively. The total content of the four main flavonoids was 14.015 1-22.871 7 mg/g. In seeds bran, the content ranges of rutin, kaempferol-3-rutinoside, quercetin and kaempferol were 4.269 0-12.118 9 mg/g, 0.258 5-1.014 9 mg/g, 0.061 8-0.735 4 mg/g, 0 -0.026 3 mg/g, respectively. The total content of the four main flavonoids was 4.651 1-13.148 6 mg/g. The results indicated that whether in seed powder or bran, rutin is the flavonoid of the highest contents, followed by kaempferol-3-rutinoside and quercetin, with kaempferol levels the lowest, less than one in ten thousandth. This study provides some important evidences for the use of certain kinds of buckwheat.

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备注/Memo

备注/Memo:
中国科学院“西部之光”博士项目(科发人教字2011-1号)和国家自然科学基金项目(21002098)资助 Supported by the ‘Western Light’ Research Program of Chinese Academy of Sciences (2011-1) and the National Natural Science Foundation of China (21002098)
更新日期/Last Update: 2015-06-23