|本期目录/Table of Contents|

[1]唐佳频,邵宗泽,张智涛,等.南极土壤来源的恶臭假单胞菌1A00316抗南方根结线虫的机制[J].应用与环境生物学报,2014,20(06):1045-1051.[doi:10.3724/SP.J.1145.2014.05009]
　TANG Jiapin,SHAO Zongze,ZHANG Zhitao,et al.Mechanism of antagonistic bacteria Pseudomonas putida 1A00316 from the South Pole soil against Meloidogyne incongnita[J].Chinese Journal of Applied & Environmental Biology,2014,20(06):1045-1051.[doi:10.3724/SP.J.1145.2014.05009]
点击复制

南极土壤来源的恶臭假单胞菌1A00316抗南方根结线虫的机制()
分享到：

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年06期
页码:
1045-1051
栏目:
研究论文
出版日期:
2014-12-31

文章信息/Info

Title:
Mechanism of antagonistic bacteria Pseudomonas putida 1A00316 from the South Pole soil against Meloidogyne incongnita
作者:
唐佳频;  邵宗泽;  张智涛;  李光玉;  景雪萍;  喻子牛;  张吉斌
1华中农业大学农业微生物学国家重点实验室，生命科学技术学院，微生物农药国家工程研究中心 武汉 430070 2国家海洋局第三海洋研究所，海洋生物遗传资源重点实验室 厦门 361005
Author(s):
TANG Jiapin;  SHAO Zongze;  ZHANG Zhitao;  LI Guangyu;  JING Xueping;  YU Ziniu;  ZHANG Jibin
1State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, National Engineering Research Center for Microbial Pesticides, Huazhong Agricultural University, Wuhan 430070, China 2Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography State Oceanic Administration, Xiamen 361005, China
关键词:
南方根结线虫; 恶臭假单胞菌; 南极土壤; 生物防治; 诱导系统抗性
Keywords:
Meloidogyne incongnita;  Pseudomonas putida;  biological control;  induced systemic resistance
分类号:
S476 : S154.39
DOI:
10.3724/SP.J.1145.2014.05009
文献标志码:
A
摘要:
对1 432株海洋和极地来源的细菌进行抗南方根结线虫的离体筛选，并对其中活性较好的一株菌进行菌种鉴定并通过盆栽实验验证其在实际生防中的效果. 设计诱导系统抗性实验，测定植物防御酶活的变化，初步研究细菌的抗虫机制. 结果显示，从1 432株细菌离体筛选得到了9株具有抗南方根结线虫的细菌，其中南极土壤来源的菌株1A00316在离体和活体中均表现出较好的抗性，其生防效果达到71.67%，经鉴定该菌株为恶臭假单胞菌（Pseudomonas putida）. 诱导番茄系统抗性实验结果显示，经菌株1A00316发酵液处理后的番茄3种防御酶苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）以及过氧化物酶（POD）的酶活均显著提高，分析数据认为该菌株的菌体可诱导番茄系统抗性. 本研究表明恶臭假单胞菌1A00316抗南方根结线虫效果较好，可作为微生物制剂应用于实际生防中；该菌不仅有直接抗虫作用，还存在间接抗虫机制，其多重抗虫机制也具有进一步的研究价值.
Abstract:
Meloidogyne incongnita causes serious damage to crops. Nowadays, attention has been paid to the side effects of chemical insecticides control. It is important to isolate the antagonistic microbe against root knot nemtaode from microbial resources. In this study 1 432 marine and polar isolates were screened against nematode to identify 9 strains with high nematocidal activity to study its biocontrol effect on nematode by pot experiments. Among them the strain 1A00316 isolated from the South Pole showed good inhibition on M. incongnita in vitro and in pot experiment, with biocontrol efficiency on nematodes as 71.67%. The experiment of induced systemic resistance (ISR) was conducted to study the possible mechanisms of 1A00316 against M. incongnita by determining the enzymes. Strain identification showed the strain 1A00316 belonged to Pseudomonas putida. The ISR mediated by strain 1A00316 in tomato clearly showed an improvement of activity of three defense enzymes: phenylalnineammonialyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) in tomato plants. The result indicated strain 1A00316 itself could induce systematic resistance to tomato and enhance their resistance against nematode. This research showed that P. putida strain 1A00316 has good resistance to M. incongnita, of both direct and induced systemic resistance, therefore can be used as microbial agents.

参考文献/References:

1 Curtis RHC. Plant parasitic nematode proteins and the host and parasite interaction [J]. Brief Funct Genomics, 2007, 6 (8): 50-58
2 Huang X, Zhao N, Zhang K. Extracellular enzymes serving as virulence factors in nematophagous fungi involved in infection of the host [J]. Res Microbiol, 2004, 115 (10): 811-816
3 Tikhonov VE, Lopez-Llorcm LV, Salinas J Jansson, HB. Puri?cation and characterization of chitinases from the nematophagous fungi Verticillium chlamydosporium and V. suchlasporium [J]. Fungal Genet Biol, 2002, 35 (1): 67-78
4 Dababat A, Sikora RA, Hauschild R. Use of Trichoderma harzianum and Trichoderma viride for the biological control of Meloidogyne incognita on tomato [J]. Commun Agric Appl Biol Sci, 2006, 71 (3): 953-961
5 Dong J, Zhou Y, Li R, Zhou W, Li L, Zhu Y, Huang R, Zhang K. New nematicidal azaphilones from the aquatic fungus Pseudohalonectria adversaria YMF1.01019 [J]. FEMS, 2006, 264 (1): 65-69
6 Khan Z, Kim SG, Jeon YH, Khanc HU, Sona SH, Kim YH. A plant growth promoting rhizobacterium, Paenibacillus polymyxa strain GBR-1, suppresses root-knot nematode [J]. Bioresour Technol, 2008, 99 (8): 3016-3023
7 Oka YJ, Chet I, Spiegel Y. Control of the root-knot nematode Meloidogyne javanica by Bacillus cereus [J]. Biocontrol Sci Technol, 1993, 3 (2): 115-126
8 Almaghrabi OA, Massoud SI, Abdelmoneim TS. Abdelmoneim. In?uence of inoculation with plant growth promoting rhizobacteria (PGPR) on tomato plant growth and nematode reproduction under greenhouse conditions [J]. Saudi J Biol Sci, 2013, 20 (1): 57-61
9 Negron JA, Acosta N. The Fusarium oxysporum f. sp. coffeae-Meloidogyne incognita complex in Bourboncoffee [J]. Nematropica, 1989, 19 (2): 161-168
10 谭可菲, 段玉玺, 朱晓峰, 罗宝君, 赵秀梅, 王连霞, 姜晓军. 根结线虫生防菌snef8对番茄诱导抗性的初步研究[J]. 黑龙江农业科学, 2011 (1): 22-24 [Tan KF, Duan YX, Zhu XF, Luo BJ, Zhao XM, Wang LX, Jiang XJ. The preliminary study on the induced systemic resistance mediated by strain snef8 against root-knot nematode in tomato. Heilongjiang Agric Sci, 2011 (1): 22-24]
11 Siddiqui IA, Shaukat SS. Suppression of root-knot disease by Pseudomonas ?uorescens CHA0 in tomato: importance of bacterial secondary metabolite,2,4-diacetylpholoroglucinol [J]. Soil Biol Biochem, 2003, 35: 1615-1623
12 Burkhead KD, Schisler DA, Slininger PJ. Pyrrolnitrin production by biological control agent Pseudomonas cepacia B37w in culture and in colonized wounds of potatoes [J]. Appl Environ Microbiol, 1994, 60 (6): 2031-2039
13 Duponnois R, Ba AM, Mateille T. Beneficial effects of Enterobacter cloacae and Pseudomonas mendocina for biocontrol of Meloidogyne incognita with the endospore-forming bacterium Pasteuria penetrans [J]. Nematologyleiden, 1999, 1: 95-102
14 Samaliev HY, Andreoglou FI, Elawad SA. The nematicidal effects of the bacteria Pseudomonas oryzihabitans and Xenorhabdus nematophilus on the root-knot nematode Meloidogyne javanica [J]. Nematology, 2000, 2 (5): 507-514
15 田宝玉, 黄薇, 江贤章, 黄建忠. 植物寄生线虫生防细菌及其作用机制研究[J]. 生物技术，2009, 19 (4): 90-93 [Tian BY，Huang H, Jiang XZ, HUANG JZ. Bacteria Used in the biocontrol of plant-parasitic nematodes and its pathogenesis [J]. Biotechnology, 2009, 19 (4): 90-93]
16 Meyer SLF, Halbrendt JM, Carta LK, Skantar AM, Liu T, Abdelnabby HME , Vinyard BT. Toxicity of 2, 4-diacetylphloroglucinol (DAPG) to plant-parasitic and bacterial-feeding nematodes [J]. J Nematol, 2009, 41 (4): 274
17 张璐, 张瑶, 刘丽丹, 曾凯芳. 膜醭毕赤酵母对草莓采后灰霉病抗病性的诱导[J]. 食品科学, 2013, 34 (22): 1-8 [Zhang L, Zhang Y, Liu LD, Zeng KF. Induction of disease resistance to gray mold by Pichia membranaefaciens in postharvest strawberry fruit. Food Sci, 2013, 34 (22): 1-8]

备注/Memo

备注/Memo:
国家重点基础研究发展计划项目（2013CB127504）资助 Supported by the National Basic Research Program of China (2013CB127504)
更新日期/Last Update: 2015-01-05