|本期目录/Table of Contents|

[1]黄琴,沈杨霞,张成静,等.铁皮石斛多酚和黄酮含量及与抗氧化活性的相关性[J].应用与环境生物学报,2014,20(03):438-442.[doi:10.3724/SP.J.1145.2014.04028]
　HUANG Qin,SHEN Yangxia,ZHANG Chengjing,et al.Correlation of the antioxidant property with the total phenolic content and total ?avonoids of different Dendrobium officinale extracts[J].Chinese Journal of Applied & Environmental Biology,2014,20(03):438-442.[doi:10.3724/SP.J.1145.2014.04028]
点击复制

铁皮石斛多酚和黄酮含量及与抗氧化活性的相关性()
分享到：

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年03期
页码:
438-442
栏目:
研究论文
出版日期:
2014-06-25

文章信息/Info

Title:
Correlation of the antioxidant property with the total phenolic content and total ?avonoids of different Dendrobium officinale extracts
作者:
黄琴; 沈杨霞; 张成静; 罗傲雪; 范益军
四川农业大学园林植物系 成都 611130
Author(s):
HUANG Qin;  SHEN Yangxia;  ZHANG Chengjing;  LUO Aoxue;  FAN Yijun
Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China
关键词:
铁皮石斛; 多酚; 黄酮; 抗氧化
Keywords:
Dendrobium officinale;  polyphenol;  ?avonoid;  antioxidant
分类号:
Q949.71+8.430.6
DOI:
10.3724/SP.J.1145.2014.04028
文献标志码:
A
摘要:
通过测试ABTS、DPPH和羟自由基的清除活性和还原能力，研究铁皮石斛不同极性提取物的体外抗氧化活性及其与多酚和黄酮含量的相关性. 采用溶剂提取法制备4个不同极性铁皮石斛提取物（EE、CE、EAE、NBE），以Vc为阳性对照. 结果显示，样品CE中总酚含量为40.96 mg/g，黄酮含量为104.48 mg/g，在各样品中含量最高. 体外抗氧化实验显示，样品CE浓度为375 μg/mL时对ABTS自由基的清除率达到100%，其IC50为88.82 μg/mL；CE浓度为1 500 μg/mL时对DPPH自由基的清除率达到78.45%，IC50为394.62 μg/mL；CE浓度为1 500 μg/mL时还原能力为62.29%，随浓度增大到3 000 μg/mL时还原能力达100%，其IC50为905.06 μg/mL. 相关性研究显示各提取物对ABTS、DPPH清除能力以及还原性与黄酮含量相关性较强，相关系数分别为0.845、0.902和0.994. 而各提取物的还原能力及对羟自由基的清除能力与样品中的多酚含量明显相关，其相关系数为0.521. 研究表明，铁皮石斛4个不同极性提取物均具有较强的体外抗氧化活性，且各提取物抗氧化活性与总酚含量和总黄酮含量之间有明显的相关性，表明铁皮石斛抗氧化活性的物质基础可能就是酚类或黄酮类成分.
Abstract:
This study aimed to understand the correlation of the antioxidant activity with the content of phenolics compounds and ?avonoids compounds in Dendrobium officinale in vitro. Four different polarity extracts (EE, CE, EAE and NBE) were prepared with different solvents. Antioxidant activity of the 4 samples was studied by reducing power assay, DPPH radical scavenging assay, ABTS radical scavenging assay and Hydroxyl radical scavenging assay with Vc as the positive control. The results indicated that CE had the highest content of phenolics compounds (40.96 mg/g) and ?avonoids compounds (104.48 mg/g). The antioxidant assays showed that CE had strong antioxidant ability on DPPH, ABTS and Hydroxyl radical. At 375 μg/mL, the scavenging rate of CE on ABTS got to 100% ( IC50 = 88.82 μg/mL). At 1 500 μg/mL, the scavenging effect on DPPH was 78.45% (IC50 = 394.62 μg/mL). The reducing power of CE was also strong, 62.29% at 1 500 μg/mL, and 100% (IC50 = 905.06 μg/mL) at 3 000 μg/mL. The results of correlation study showed that compared with the content of phenolics, the content of ?avonoids had a stronger relationship with the ABTS (r = 0.845), DPPH radical (r = 0.902) scavenging activity and the reducing power (r = 0.994), whereas the content of phenolics had a stronger relationship with Hydroxyl radical (r = 0.521) scavenging activity than the content of ?avonoids. This research indicated that there is a close relationship between the content of phenolics and ?avonoids and the antioxidant activity of all extracts, and the material basis of antioxidant activity of Dendrobium officinale may be phenolics and ?avonoids.

参考文献/References:

1 Babbs CF. Free radicals and the etiology of colon cancer[J]. Free Radical Biol Med, 1990, 8 ( 2): 191-200
2 Olas B, Wachowicz B, Mielicki WP, Buczn A. Free radicals are involved in cancer procoagulant-induced platelet activation [J]. Thromb Res, 2000, 97 (3): 169-175
3 Dreher D, Junod AF. Role of oxygen free radicals in cancer development [J]. Eur J Cancer, 1996, 32 (1): 30-38
4 魏小勇, 龙艳, 詹宇坚, 李熙灿, 徐勤, 徐传磊. 金钗石斛提取物抗白内障的体外实验研究[J]. 现代中药研究与实践, 2008, 22 (2): 27-31 [Wei XY, Long Y, Zhan YJ, Li XS, Xu Q , Xu CL. Study on the prevention of cataract in rat lens from Dendrobium nobile Lindl.extractives in vitro [J]. Res Practice Chin Med, 2008, 22 (2): 27-31]
5 范益军, 罗傲雪, 罗傲霜, 何涛, 刘静, 淳泽, 何兴金.迭鞘石斛中性多糖DDP1-1体内免疫活性研究[J]. 应用与环境生物学报, 2010, 16 (3): 376-379 [Fan YJ, Luo AX, Luo AS, He T, Liu J, Chun Z, He XJ. In vivo immunomodulatory activities of neutral polysaccharide (DDP1-1) from Dendrobium denneanum [J]. Chin J Appl Environ Biol, 2010, 16 (3): 376-379
6 Zha XQ, Luo JP, Luo SZ, Jiang ST. Structure identification of a new immunostimulating polysaccharide from the stems of Dendrobium huoshanense [J]. Carbohyd Res, 2007, 69 (1): 86-93
7 Wang JH, Luo JP, Zha XQ, Feng BJ. Comparison of antitumor activities of different polysaccharide fractions from the stems of Dendrobium nobile Lindl [J]. Carbohyd Polym, 2010, 79 (1): 114-118
8 罗傲雪, 宋关斌, 淳泽. 迭鞘石斛抗肿瘤作用研究[J]. 应用与环境生物学报, 2007, 13 (2): 184-187 [Luo AX, Song GB, Chun Z. Inhibiting effect of tumor by Dendrobium denneanum [J]. J Appl Environ Biol, 2007, 13 (2): 184-187]
9 罗傲霜, 淳泽, 葛绍荣, 罗傲雪, 范益军, 刘鹏, 彭文景. 迭鞘石斛多糖降血糖作用研究[J]. 应用与环境生物学报, 2006, 12 (3): 334-337 [Luo AS, Chun Z, Ge SR, Luo AX, Fan YJ, Liu P, Peng WJ. Effect of Dendrobium denneanum polysaccharide reducing blood glucose [J]. Chin J Appl Environ Biol, 2006, 12 (3): 334-337]
10 Fan YJ, He XJ, Zhou SD, Luo AX, Chun Z. Composition analysis and antioxidant activity of polysaccharide from Dendrobium denneanum [J]. Int J Biol Macromol, 2009, 45: 169-173
11 鲍素华, 查学强, 郝杰, 罗建平. 不同分子量铁皮石斛多糖体外抗氧化活性研究[J]. 食品科学, 2009, 30 (21): 123-127 [Bao SH, Zha XQ, Hao J, Luo JP. In vitro antioxidant activity of polysaccharides with different molecular weights from Dendrobium candidum [J]. Food Sci, 2009, 30 (21): 123-127]
12 李玲, 邓晓兰, 赵兴兵, 曾勇, 欧阳冬生. 铁皮石斛化学成分及药理作用研究进展[J]. 肿瘤药学, 2011, 1 (2): 25-45 [Li L, Deng XL, Zhao XB, Zeng Y, Ouyang DS. Progress in chemical constituents and pharmacological effects of Dendrobium officinale [J]. Oncol Pharm, 2011, 1 (2): 25-45]
13 Li Y, Wang CL, Wang YJ, Guo SX, Yang JS, Chen XM, Xiao PG. Three new bibenzyl derivatives from Dendrobium candidum [J]. Chem Pharm Bull, 2009, 57 (2): 218-219
14 李燕, 王春兰, 王芳菲, 董海玲, 郭顺星, 杨峻山, 肖培根. 铁皮石斛中的酚酸类及二氢黄酮类成分[J]. 中国药学杂志, 2010, 45 (13): 975-980 [Li Y, Wang CL, Dong HL, Guo SX, Yang JS, Xiao PG. Phenolic components and flavanones from Dendrobium candidum [J]. China J Chin Mat Med, 2010, 45 (13): 975-980]
15 Terpinc P, ˇCeh B, Ulrih NP, Abramoviˇc H. Studies of the correlation between antioxidant properties and the total phenolic content of different oil cake extracts [J]. Ind Crops Prod, 2012, 39: 210-217
16 Gutfinger T. Polyphenols in olive oils [J]. J Am Oil Chem Soc, 1981, 58: 966-968
17 Sakanaka S, Tachibana Y, Okada Y. Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha) [J]. Food Chem, 2005, 89: 569-575
18 Re R., Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay[J]. Free Rad Biol Med, 1999, 26 (9-10): 1231-1237
19 Luo AX, Fan YJ. Antioxidant activities of berberine hydrochloride [J]. J Med Plants Res, 2011, 5: 3702-3707
20 Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion [J]. J Agr Food Chem, 1992, 40 (6): 945-948
21 Gyamfi MA, Yonamine M, Aniya Y. Free radical scavenging activity of medicinal herb of Ghana: Thonningia sanguinea on experimentally induced liver injuries [J]. General Pharmacol, 1999, 32 (6): 661-667
22 Fan YJ, Ge ZF, Luo AX. In vitro antioxidant activity of polysaccharide from Gardenia jasminoides Ellis [J]. J Med Plants Res, 2011, 5 (14): 2963-2968
23 Yen GY, Chen HY. Antioxidant activity of various tea extracts in relation to their antimutagenicity [J]. J Agric Food Chem, 1995, 43: 27-32
24 Petkovsek MM, Slatnar A, Stampar F. The influence Of organic/integrated production on the content of phenolic compounds in apple leaves and fruits in four different varieties over a 2-year period [J]. J Sci Food Agric, 2010, 90 (14): 2366-2378
25 张文华, 曹明容. 常见分类化合物抗油脂氧化活性-结构关系理论研究[J]. 皮革科学与工程, 2010, 20 (5): 10-15 [Zhang WH, Cao MR. Theoretical study on the relationship between structure and antioxidative activity of typical phenols in lipid [J]. Leather Sci Eng, 2010, 20 (5): 10-15]
26 黄池宝, 罗宗铭, 宾丽英.黄酮类化合物抗氧化性与其结构关系的研究[J]. 广东工业大学学报, 2000, 17 (2): 71-75 [Huang CB, Luo ZM, Bin LY. Study on the relation between structure and antioxygenic activity of flavonoid [J]. J Univ Technol, 2000, 17 (2): 71-75]

相似文献/References:

[1]杨雪飞,王瑛,罗建平.铁皮石斛外源lea3基因的转化及耐盐性分析[J].应用与环境生物学报,2010,16(05):622.[doi:10.3724/SP.J.1145.2010.00622]
　YANG Xuefei,WANG Ying,LUO Jianping.Transformation of lea3 Gene into Dendrobium candidum Wall. ex Lindl for Enhancing Its Salt Tolerance[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):622.[doi:10.3724/SP.J.1145.2010.00622]
[2]孙晶,何涛,万闰兰,等.铁皮石斛尿苷二磷酸葡萄糖焦磷酸化酶基因（UGP）的密码子偏好性分析[J].应用与环境生物学报,2014,20(05):759.[doi:10.3724/SP.J.1145.2014.03041]
　SUN Jing,HE Tao,WAN Runlan,et al.The Codon usage bias of UDP-glucose pyrophosphorylase gene (UGP) in Dendrobium officinale[J].Chinese Journal of Applied & Environmental Biology,2014,20(03):759.[doi:10.3724/SP.J.1145.2014.03041]
[3]赵兴兵,吴维佳,肖嫩群,等.超微铁皮石斛对脾虚便秘小鼠肠道细菌分子多样性的影响[J].应用与环境生物学报,2015,21(01):170.[doi:10.3724/SP.J.1145.2013.12035]
　ZHAO Xingbing,WU Weijia,XIAO Nenqun,et al.Effect of ultra-micro powder Dendrobium officinale on the molecular diversity of intestinal bacteria in mice with spleen-deficiency constipation[J].Chinese Journal of Applied & Environmental Biology,2015,21(03):170.[doi:10.3724/SP.J.1145.2013.12035]
[4]费雯,淳泽,何沁嶷,等.金钗石斛总多酚提取工艺及体外抗氧化活性[J].应用与环境生物学报,2015,21(04):623.[doi:10.3724/SP.J.1145.2015.01047]
　FEI Wen,CHUN Ze,HE Qinyi,et al.Extraction conditions and in vitro antioxidant activities of polyphenols from Dendrobium nobile[J].Chinese Journal of Applied & Environmental Biology,2015,21(03):623.[doi:10.3724/SP.J.1145.2015.01047]
[5]莫雨轩,张浩涵,田红怡,等.蚯蚓粪对铁皮石斛抗氧化能力及次生代谢产物的影响?[J].应用与环境生物学报,2017,23(04):642.[doi:10.3724/SP.J.1145.2016.09008]
　MO Yuxuan,ZHANG Haohan,TIAN Hongyi,et al.Synthesis and antibacterial activity of flavonols[J].Chinese Journal of Applied & Environmental Biology,2017,23(03):642.[doi:10.3724/SP.J.1145.2016.09008]
[6]范益军,淳泽,梅娟娟,等.镧在UV-B应力下对迭鞘石斛生理及药用成分的影响[J].应用与环境生物学报,2020,26(05):1218.[doi: 10.19675/j.cnki.1006-687x.2019.10003]
　FAN Yijun,CHUN Ze,MEI Juanjuan,et al.Effects of lanthanum on physiology and medicinal components of Dendrobium?aurantiacum?Rchb. f. var. denneanum (Kerr) under UV-B stress[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):1218.[doi: 10.19675/j.cnki.1006-687x.2019.10003]
[7]乔自鹏,张君毅,王明元,等.一株胜红蓟内生真菌Letendraea sp. WZ07的鉴定、培养条件与抗氧化活性[J].应用与环境生物学报,2021,27(02):449.[doi:10.19675/j.cnki.1006-687x.2020.09001]
　QIAO Zipeng,ZHANG Junyi,WANG Mingyuan,et al.Identification of endophytic fungi Letendraea sp. WZ07 from Ageratum conyzoides and its fermentation extract antioxidant activity[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):449.[doi:10.19675/j.cnki.1006-687x.2020.09001]
[8]赵桦,张舒婷,刘蒲东,等.石斛PRMT基因家族的鉴定及其在不同光周期下的表达[J].应用与环境生物学报,2022,28(02):413.[doi:10.19675/j.cnki.1006-687x.2020.10060]
　ZHAO Hua,ZHANG Shuting,LIU Pudong,et al.Genome-wide identification of PRMT gene family and expression analysis under different photoperiods in Dendrobium[J].Chinese Journal of Applied & Environmental Biology,2022,28(03):413.[doi:10.19675/j.cnki.1006-687x.2020.10060]

备注/Memo

备注/Memo:
大学生创新实验计划项目（121062655）和四川省教育厅重点项目（12ZA274）资助 Supported by the National Innovation Experiment Program for University Students (121062655) and the Education Department Foundation of Sichuan Province ( 12ZA274)
更新日期/Last Update: 2014-07-01