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[1]李仁强,靳艳玲,郜晓峰,等.硫酸亚铁促进Clostridium acetobutylicum CICC8012发酵麦秸水解液产丁醇[J].应用与环境生物学报,2015,21(03):447-454.[doi:10.3724/SP.J.1145.2014.04012]
 LI Renqiang,JIN Yanling,GAO Xiaofeng,et al.Ferrous sulfate improving buanol production of wheat straw hydrolysate by Clostridium acetobutylicum CICC8012[J].Chinese Journal of Applied & Environmental Biology,2015,21(03):447-454.[doi:10.3724/SP.J.1145.2014.04012]
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硫酸亚铁促进Clostridium acetobutylicum CICC8012发酵麦秸水解液产丁醇()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年03期
页码:
447-454
栏目:
研究论文
出版日期:
2015-06-25

文章信息/Info

Title:
Ferrous sulfate improving buanol production of wheat straw hydrolysate by Clostridium acetobutylicum CICC8012
作者:
李仁强靳艳玲郜晓峰张国华赵海
1中国科学院成都生物研究所 成都 610041 2中国科学院大学 北京 100049 3中国科学院环境与应用微生物重点实验室 成都 610041 4河南大学药学院 开封 475001
Author(s):
LI Renqiang JIN Yanling GAO Xiaofeng ZHANG Guohua ZHAO Hai
1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2University of Chinese Academy of Sciences, Beijing 100049, China 3Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 4College of Pharmacy, Henan University, Kaifeng 475001, China
关键词:
丙酮丁醇梭菌纤维素水解液硫酸亚铁原位脱毒燃料丁醇
Keywords:
Clostridium acetobutylicum CICC8012 cellulosic hydrolysate ferrous sulfate in situ detoxification fuel butanol
分类号:
TQ923
DOI:
10.3724/SP.J.1145.2014.04012
文献标志码:
A
摘要:
以农业废弃物小麦秸秆为原料,以丙酮丁醇梭菌Clostridium acetobutylicum CICC8012为菌种发酵生产丁醇,通过单因素实验和响应面优化设计,优选硫酸亚铁对水解液进行原位脱毒,优化发酵条件并验证硫酸亚铁原位脱毒对多种原料的有效性. 发酵结果显示,伴随发酵液残糖含量显著降低,溶剂产量和发酵效率显著提高. 在优化的发酵条件下,初始糖浓度50 g/L,接种量10.5%,硫酸亚铁浓度0.4 g/L,发酵65 h,丁醇产量达7.33 g/L,与优化模型预测值7.35 g/L的最大丁醇产量接近,模型显著可靠. 对稻草水解液、玉米秸秆水解液、标准水解液等不同原料进行处理,丁醇和总溶剂产量均得到显著提高. 本研究证明直接添加硫酸亚铁对秸秆水解液丁醇发酵具有显著促进作用,很可能是一种潜在的通用增效方法,可用于提高纤维素水解液的丁醇产量.
Abstract:
Wheat straw, a common agricultural residual, was utilized in this study as raw material to produce solvents including acetone, ethanol and butanol (ABE). To remove the inhibition of the inhibitors in the wheat straw hydrolysate, ferrous sulfate was used for in situ detoxification of the fermentation medium, resulting in improved solvent production and fermentation efficiency, together with reduced residual sugar and intermediates concentrations. Four crucial factors including sugar concentration, inoculum ratio, ferrous sulfate concentration and fermentation time were chosen to optimize fermentation conditions with response surface methodology. The optimal conditions predicted by the model were sugar concentration of 50 g/L, inoculum ratio of 10%, ferrous sulfate concentration of 0.4 g/L and fermentation time of 65 h, to obtain butanol concentration of 7.35 g/L. The verification experiment achieved a butanol concentration of 7.33 g/L, indicating the reliability of the model. Additionally, rice straw and corn straw hydrolysate media were also detoxified with ferrous sulfate to obtain improvement similar to wheat straw hydrolysate, showing the extensive effectiveness of the detoxification method.

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备注/Memo

备注/Memo:
中国科学院“西部之光”项目(Y2C5021100)资助 Supported by the Western Light Talent Cultivation Program of Chinese Academy of Sciences (Y2C5021100)
更新日期/Last Update: 2015-06-23