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[1]吴限,高会,周世瑶,等.海洋沉积物中六溴环十二烷的微生物降解特性及降解菌的分离鉴定[J].应用与环境生物学报,2014,20(06):1070-1075.[doi:10.3724/SP.J.1145.2014.03019]
 WU Xian,GAO hui,ZHOU Shiyao,et al.Characteristics of hexabromocyclododecane (HBCDs) degradation by marine sediment microbes and the isolation and identification of degradation bacteria[J].Chinese Journal of Applied & Environmental Biology,2014,20(06):1070-1075.[doi:10.3724/SP.J.1145.2014.03019]
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海洋沉积物中六溴环十二烷的微生物降解特性及降解菌的分离鉴定()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年06期
页码:
1070-1075
栏目:
研究论文
出版日期:
2014-12-31

文章信息/Info

Title:
Characteristics of hexabromocyclododecane (HBCDs) degradation by marine sediment microbes and the isolation and identification of degradation bacteria
作者:
吴限 高会 周世瑶 张婉茹 党红蕾 邱丽娜 高艳飞 祖国仁 那广水
1大连工业大学生物工程学院 大连 116034 2国家海洋环境监测中心,国家海洋局近岸海域生态环境重点实验室 大连 116023 3大连科技学院 旅顺 116052
Author(s):
WU Xian GAO hui ZHOU Shiyao ZHANG Wanru DANG Honglei QIU Lina GAO Yanfei ZU Guoren NA Guangshui
1Dalian Polytechnic University, Dalian 116034, China 2Key Laboratory of coastal ecology and environment of State Oceanic Administration, National Marine Environmental Monitoring Center, Dalian 116023, China 3Dalian Institute of Science and Technology, Lüshun, 116052, china
关键词:
HBCDs海洋沉积物微生物降解降解菌株
Keywords:
HBCDs marine sediment microbial degradation degradation strain
分类号:
X172 : X55
DOI:
10.3724/SP.J.1145.2014.03019
文献标志码:
A
摘要:
为了解六溴环十二烷(HBCDs)在海洋沉积物中的好氧微生物降解特性,在以HBCDs为唯一碳源的无机培养基中添加海洋沉积物悬浮液,利用HPLC-MS/MS测定HBCDs降解率,并驯化分离得到HBCDs降解菌株,进行菌种鉴定. 结果显示:海洋沉积物好氧微生物对HBCDs的降解半衰期为38-61 d;在120 d中HBCDs共降解了72%;从海洋沉积物中富集、分离得到5株能够以HBCDs为唯一碳源的降解菌株,分别命名为CTF711-A、CTF711-C、CTF711-D、CTF711-E和CTF711-X,结合形态学观察、生理生化试验和16S rDNA序列分析,将其依次鉴别为假单胞菌属、红球菌属、不动杆菌属、微杆菌属和盐单胞菌属,除假单胞菌属外,其余菌株对HBCDs的降解能力还尚未见报道. 同时将这5株菌分别培养与混合培养,混合菌株对HBCDs降解率最高. 本研究报道了5株不同属的HBCDs好氧降解菌株,且混合菌株对HBCDs降解效果较好,结果可为HBCDs的生物修复提供理论和应用依据.
Abstract:
In order to study the characteristics of aerobic microbial degradation of hexabromocyclododecane (HBCDs) in marine sediments, the degradation rate of HBCDs was investigated using HPLC-MS/MS. The suspension of marine sediment was added to inorganic mediums with HBCDs as the sole carbon source. The HBCDs degradation strains were isolated after domestication, then molecular identification was performed. The results showed the half-life of HBCDs degradation by aerobic microorganisms in marine sediment as 31-62 days. Five strains of bacteria were isolated and named CTF711-A, CTF711-C, CTF711-D, CTF711-E, and CTF711-X, respectively. Based on morphology, biochemical tests and 16S rDNA sequence analysis, the five strains were classified as Pseudomonas, Rhodococcus, Acinetobacter, Microbacterium and Halomonas. The degrading rate of mixed bacteria strains was higher than that of single strains. The results provided supporting data for HBCDs bioremediation of contaminated environment.

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备注/Memo

备注/Memo:
1Dalian Polytechnic University, Dalian 116034, China 2Key Laboratory of coastal ecology and environment of State Oceanic Administration, National Marine Environmental Monitoring Center, Dalian 116023, China 3Dalian Institute of Science and Technology, Lüshun, 116052, china
更新日期/Last Update: 2015-01-05