|本期目录/Table of Contents|

[1]周阳,彭银仙,刘朝莹,等. 集胞藻PCC 6803染色体上relNEs位点TA系统编码产物对蓝藻细胞生长的影响[J].应用与环境生物学报,2014,20(05):934-937.[doi:10.3724/SP.J.1145.2014.01045]
 ZHOU Yang,PENG Yinxian,LIU Zhaoying,et al. Effect of proteins encoded by chromosomal TA locus relNEs on growth of Syenchocystis PCC 6803[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):934-937.[doi:10.3724/SP.J.1145.2014.01045]
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 集胞藻PCC 6803染色体上relNEs位点TA系统编码产物对蓝藻细胞生长的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年05期
页码:
934-937
栏目:
研究简报
出版日期:
2014-10-25

文章信息/Info

Title:
 Effect of proteins encoded by chromosomal TA locus relNEs on growth of Syenchocystis PCC 6803
作者:
 周阳彭银仙刘朝莹赵卫飞
 1江苏科技大学生物与化学工程学院 镇江 212013
2江苏大学环境与安全工程学院 镇江 212013
Author(s):
 ZHOU Yang PENG Yinxian LIU Zhaoying ZHAO Weifei
 1College of Biological and Chemical engineering, Jiangsu University of Science and Technology, Zhenjiang 212013, China
2College of Environmental and Safety Engineering, Jiangsu University, Zhenjiang 212013, China
关键词:
 集胞藻PCC 6803Cu2+诱导启动子TA系统relNEs位点
Keywords:
 Synechocystis PCC 6803 copper ion-inducible promoter TA system relNEs locus
分类号:
Q75 : Q945.78
DOI:
10.3724/SP.J.1145.2014.01045
文献标志码:
A
摘要:
细菌毒素-抗毒素(Toxin-antitoxin,TA)系统最重要的特点是毒素蛋白具有生长抑制作用,抗毒素蛋白能拮抗这种生长抑制作用. 为证明蓝藻细胞染色体上relNEs系统的生理功能,利用受Cu2+诱导的启动子PpetE控制集胞藻PCC 6803染色体上公认的TA系统位点relNEs基因的表达调控突变株,分析编码产物对蓝藻细胞生长的影响. 结果显示:Cu2+诱导毒素基因relEs过表达,藻细胞生长明显受到抑制,而Cu2+诱导毒素抗毒素基因relNEs共表达的菌株(DR381-486)则可以正常生长. 研究结果表明诱导relEs表达产物能抑制自源宿主集胞藻细胞的生长,而RelN能拮抗RelEs的这种生长抑制作用,进一步证明集胞藻染色体上的操纵子构成rel家族TA系统.
Abstract:
A crucial characteristics of bacterial toxin-antitoxin (TA) systems is the growth inhibition effect of the toxins, which can be neutralize by their cognate antitoxins. This study aimed to prove the physiological function of relNEs TA systerm on the chromosome of Synechocystis PCC6803. We constructed the regulation mutants where the Cu2+-inducible promoter PpetE was used to control expression of the TA system relNEs on the chromosome of Synechocystis PCC6803. Growth of the mutants was analyzed in the absence or presence of copper ion. The results showed that under the background of deletion of relNEs system, the over expression of relEs induced with copper ion significantly inhibited algae cell growth, and that the coexpression of relNEs restored cell growth. These results showed that expression of the gene relEs causes growth arrest of cyanobacteria cells, and that RelN can counteract this inhibition effect of RelEs, further demonstrating that the operon relNEs forms a functional TA system of the rel TA family.

参考文献/References:

 

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备注/Memo

备注/Memo:
 国家自然科学基金项目(30771176)资助 Supported by the National Natural Science Foundation of China (30771176)
更新日期/Last Update: 2014-10-30