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[1]黄天培,张君,戴瑞卿,等.5个杀虫调控基因对苏云金芽胞杆菌407解毒Cr(Ⅵ)的影响[J].应用与环境生物学报,2014,20(05):785-790.[doi:10.3724/SP.J.1145.2014.01023]
 HUANG Tianpei,ZHANG Jun,DAI Ruiqing,et al.Effects of 5 insecticidal regulatory genes on Cr(Ⅵ) detoxification of Bacillus thuringiensis 407[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):785-790.[doi:10.3724/SP.J.1145.2014.01023]
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5个杀虫调控基因对苏云金芽胞杆菌407解毒Cr(Ⅵ)的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年05期
页码:
785-790
栏目:
研究论文
出版日期:
2014-10-25

文章信息/Info

Title:
Effects of 5 insecticidal regulatory genes on Cr(Ⅵ) detoxification of Bacillus thuringiensis 407
作者:
黄天培 张君 戴瑞卿 潘洁茹 张灵玲 关雄
1福建农林大学生物农药与化学生物学教育部重点实验室 福州 350002 2福州市疾病预防控制中心 福州 350004
Author(s):
HUANG Tianpei ZHANG Jun DAI Ruiqing PAN Jieru ZHANG Lingling GUAN Xiong
1Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2Fuzhou Center for Disease Control and Prevention, Fuzhou 350004, China
关键词:
苏云金芽胞杆菌解毒Cr(Ⅵ)Cr(Ⅵ)还原Cr(Ⅵ)抗性调控基因
Keywords:
Bacillus thuringiensis detoxification Cr(Ⅵ) Cr(Ⅵ) reduction Cr(Ⅵ) resistance regulatory gene
分类号:
S476 : X172
DOI:
10.3724/SP.J.1145.2014.01023
文献标志码:
A
摘要:
测定苏云金芽胞杆菌(Bacillus thuringiensis,简称Bt)407及其突变株407 ΔplcR、407 ΔinhA2、407 ΔmpbE、407 ΔsigmaE和407 ΔsigmaK对数生长期与稳定期的拐点时间t0,测定不同时间点发酵液上清中的总铬(Cr)和Cr(Ⅵ)的浓度,并分析Bt 407基因组的抗性相关基因,进而初步构建Bt解毒Cr(Ⅵ)模式图. 结果表明,plcR、inhA2、mpbE、sigmaK负调控Bt 407解毒Cr(Ⅵ)的能力并主要影响Cr(Ⅵ)还原过程,sigmaE不影响对Cr(Ⅵ)的解毒过程. 利用Bt 407基因组序列分析得到与Cr(Ⅵ)抗性相关基因NADPH偶氮还原酶基因azoR、外排蛋白基因efx和chrA. 推测Bt 407对Cr(Ⅵ)抗性机制可能为:azoR编码NADPH偶氮还原酶将细胞内Cr(Ⅵ)还原为Cr(Ⅲ),Cr(Ⅲ)再通过ChrA或与azoR共表达的外排蛋白Efx从细胞质中排出,这一还原过程直接/间接受到plcR、inhA2、mpbE、sigmaK等调控基因的负调控. 因此,Bt 407对Cr(Ⅵ)的解毒机制是由azoR、efx、chrA等主效基因与plcR、inhA2、mpbE、sigmaK等调控基因共同作用的复杂网络.
Abstract:
This study aimed to investigate the effects of insecticidal regulatory genes on Cr(Ⅵ) detoxification of Bacillus thuringiensis 407. The growth curve of Bt 407 and its mutants (407 ΔplcR, 407 ΔinhA2, 407 ΔmpbE, 407 ΔsigmaE and 407 ΔsigmaK) were applied to determine the t0 (time of the strain enter into the stationary phase). The Cr(VI) and total Cr concentrations at t-3 (3 h before t0), t-2 (2 h before t0), t-1 (1 h before t0), t0, t1 (1 h after t0), t2 (2 h after t0), t3 (3 h after t0) and t4 (4 h after t0) were also detected. The genomic sequence of Bt 407 was analyzed for the existence of Cr(Ⅵ) resistant genes. And the model chart of Bt 407 Cr(Ⅵ) detoxification was primarily constructed. The results showed that PlcR, InhA2, MpbE and SigmaK negatively regulated the detoxification of Cr(Ⅵ) by reducation, while SigmaE had no effects on the process. Cr(Ⅵ) resistant genes azoR, efx and chrA were obtained by genomic analysis of Bt 407. The mechanisms of Cr(Ⅵ) detoxification by Bt 407 was proposed as follows: AzoR reduces Cr(Ⅵ) to Cr(Ⅲ), which is negatively regulated by PlcR, InhA2, MpbE and SigmaK. The efflux of chromium was conferred by antiporters Efx and ChrA. These results suggested that the resistance of Bt 407 to Cr(Ⅵ) is a complicated network regulated by azoR, efx, chrA, plcR, inhA2, mpbE and sigmaK genes.

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备注/Memo

备注/Memo:
“十二五”农村领域国家科技计划课题(2011AA10A203)、国家自然科学基金项目(31201574,31071745)和福建省教育厅高校领军人才项目(k8012012a)资助 Supported by the National Science and Technology Project for Rural Development of the Twelfth Five-Year-Plan of China (2011AA10A203), the National Natural Science Foundation of China ( 31201574, 31071745) and the Leading Talents of Fujian Province Colleges (k8012012a)
更新日期/Last Update: 2014-10-29