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[1]张斌,邱志刚,薛斌,等.聚糖菌颗粒污泥的反硝化特性与微生物群落分布[J].应用与环境生物学报,2014,20(04):551-557.[doi:10.3724/SP.J.1145.2013.12047]
 ZHANG Bin,QIU Zhigang,XUE Bin,et al.Denitrification characteristics and community distribution of glycogen accumulating organism granules[J].Chinese Journal of Applied & Environmental Biology,2014,20(04):551-557.[doi:10.3724/SP.J.1145.2013.12047]
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聚糖菌颗粒污泥的反硝化特性与微生物群落分布()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年04期
页码:
551-557
栏目:
研究论文
出版日期:
2014-08-25

文章信息/Info

Title:
Denitrification characteristics and community distribution of glycogen accumulating organism granules
作者:
张斌 邱志刚 薛斌 谌志强 龚泰石 王雪 智维佳 王景峰
1军事医学科学院卫生学环境医学研究所 天津 300050 2天津市环境与食品安全风险监控技术重点实验室 天津 300050
Author(s):
ZHANG Bin QIU Zhigang XUE Bin CHEN Zhiqiang GONG Taishi WANG Xue ZHI Weijia WANG Jingfeng
1Institute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China 2Tianjin Key Laboratory of Risk Assessment and Control for Environment & Food Safety, Tianjin 300050, China
关键词:
聚糖菌反硝化颗粒污泥微生物群落动态生物脱氮气态产物四联球菌
Keywords:
glycogen-accumulating organisms (GAOs) denitrification granular sludge microbial community development biological nitrogen removal gaseous product tetrad-forming organisms (TFOs)
分类号:
X703 : X172
DOI:
10.3724/SP.J.1145.2013.12047
文献标志码:
A
摘要:
为了解聚糖菌在污泥颗粒化中的脱氮能力及其微生物生态特性,采用反应器工艺、批式试验、显微技术和荧光原位杂交技术来评估其反硝化能力,揭示其微生物群落的微观结构,探索聚糖菌和聚磷菌在不同粒径污泥中的分布特征. 结果表明,污泥对有机物的吸收率稳定在90%以上. 颗粒污泥的沉降指数(SVI10)稳定在30-50 mL g-1,远低于接种污泥的108.2 mL g-1. 聚醣菌颗粒污泥对硝酸盐氮和亚硝酸盐氮的反硝化去除率分别达到了65%和70%,氮气为主要气态产物. 聚糖菌颗粒污泥被大量的四联球菌结构所充斥,丝状菌在其中起到了重要的构架和搭桥的作用. 荧光原位杂交结果表明聚糖菌可生存于各尺寸颗粒化污泥内;而聚磷菌受到严重抑制只能较少地分布在颗粒污泥的外围空间. 上述结果表明,在SBR反应器中采用厌氧搅拌—排水—好氧曝气的处理模式成功培育出具有聚糖特性的颗粒化微生物聚集体,培育成功的颗粒污泥对硝酸盐氮和亚硝酸盐氮均具有良好的反硝化特性. 在整体颗粒污泥微生物群落中聚糖菌为优势种群. 图8 表1 参19
Abstract:
This study aimed to investigate the denitrification ability and microbial ecological characteristic of glycogen-accumulating organisms (GAOs) during sludge granulation. A combined method of reactor process, batch test, microscale techniques and fluorescence in situ hybridization (FISH) was employed to evaluate the denitrification ability of GAOs, to reveal the microstructure of microbial communities and to characterize the distribution of GAOs and polyphosphate-accumulating organisms (PAOs) in different size granules. The results indicated that the adsorption rate of organic compound by sludge was over 92%. The SVI10 of granules stabilized at 30-50 mL g-1, a very low value compared to those measured for inoculating sludge (108.2 mL g-1). The nitrogen removal rate of nitrate and nitrite by GAOs granules reached 65% and 70% respectively, with N2 as the main gaseous product. The granules were tightly packed with a large amount of tetrad-forming organisms (TFOs), with filamentous bacteria serving as the supporting structures for the granules. FISH demonstrated that GAOs were distributed evenly throughout the granular space, whereas PAOs were severely inhibited and presented only in the edge of granules. A lab-scale sequencing batch reactor (SBR) could be successfully operated for cultivating GAOs granules in alternating anaerobic stirring-discharge-aerobic modes, with the GAOs granules showing excellent denitrifying capability. Therefore GAOs are the predominant member of the microbial population in granules.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目(51108456,50908227)、国家863计划项目(2009AA06Z312)资助 Supported by the National Natural Science Foundation of China (51108456, 50908227) and the National High Technology Research and Development Program of China (2009AA06Z312)
更新日期/Last Update: 2014-08-26