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[1]郭抗萧,谭周进,谢梦洲,等.超微七味白术散与酵母菌协同治疗小鼠菌群失调腹泻[J].应用与环境生物学报,2015,21(01):61-67.[doi:10.3724/SP.J.1145.2013.10002]
 GUO Kangxiao,TAN Zhoujin,XIE Mengzhou,et al.The synergic effect of ultra-micro powder Qiweibaizhusan combined with yeast on dysbacteriotic diarrhea mice[J].Chinese Journal of Applied & Environmental Biology,2015,21(01):61-67.[doi:10.3724/SP.J.1145.2013.10002]
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超微七味白术散与酵母菌协同治疗小鼠菌群失调腹泻()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
21卷
期数:
2015年01期
页码:
61-67
栏目:
研究论文
出版日期:
2015-02-25

文章信息/Info

Title:
The synergic effect of ultra-micro powder Qiweibaizhusan combined with yeast on dysbacteriotic diarrhea mice
作者:
郭抗萧 谭周进 谢梦洲 佘颜 王学红
1湖南中医药大学 长沙 410208 2长沙卫生职业学院 长沙 410100 3中南大学湘雅二医院消化内科 长沙 410011
Author(s):
GUO Kangxiao TAN Zhoujin XIE Mengzhou SHE Yan WANG Xuehong
1Hunan University of TCM, Changsha 410208, China 2Changsha Health Vocational College, Changsha 410100, China 3Department of Gastroenterology, Xiangya Second Hospital, Central South University, Changsha 410011, China
关键词:
超微七味白术散酵母菌菌群失调肠道微生物肠道酶
Keywords:
ultra-micro powder Qiweibaizhusan yeast dysbacteriotic intestinal microorganisms intestinal enzyme
分类号:
R285.5
DOI:
10.3724/SP.J.1145.2013.10002
文献标志码:
A
摘要:
探讨超微七味白术散与酵母菌联用对菌群失调腹泻的疗效,可为超微七味白术散与酵母菌配伍制剂的研发提供依据. 采用抗生素建立小鼠菌群失调腹泻模型,灌胃给药七味白术散50%量超微汤药、50%量超微汤药+12.5%量酵母、25%量超微汤药+25%量酵母、12.5%量超微汤药+50%量酵母及全量酵母,比较分析小鼠体重变化率、免疫器官指数、肠道菌群数及酶活. 结果表明,超微七味白术散和酵母菌对小鼠体重和脾脏无显著影响,各治疗组的胸腺指数为正常水平(P > 0.05);超微七味白术散和酵母菌均能促进双歧杆菌和乳酸菌的生长(P < 0.01),调节微生态平衡;50%量超微汤药组和25%量超微汤药+25%量酵母组的大肠杆菌、细菌数恢复至正常水平(P > 0.05). 治疗后肠道内纤维素酶活性均恢复至正常组(对照)水平(P > 0.05);50%量超微汤药+12.5%量酵母组和25%量超微汤药+25%酵母量组的淀粉酶活性恢复至正常水平(P > 0.05);木聚糖酶、蛋白酶活性与超微饮片的浓度及酵母菌的量有一定的关系,但均未达到正常组水平(P < 0.05). 说明七味白术散25%量超微汤药+25%量酵母配伍能够有效治疗菌群失调小鼠腹泻,其效果与超微七味白术散50%量相当,达到全量传统汤剂的效果.
Abstract:
This research aimed to provide scientific basis for preparation development of ultra-micro powder Qiweibaizhusan combined with yeast by exploring its clinic effect on dysbacteriotic diarrhea. First, dysbacteriotic diarrheal mice model was constructed via administration of antibiotics. The modeled mice were treated with 50% amount of ultra-micro Qiweibaizhusan, 50% amount of ultra-micro Qiweibaizhusan + 12.5% amount of yeast, 25% amount of ultra-micro Qiweibaizhusan + 25% amount of yeast, 12.5% amount of ultra-micro Qiweibaizhusan + 50% amount of yeast, and yeast alone, respectively. The body weight, spleen weight, thymus weight, intestinal microbiota and enzyme activities were analyzed. The results showed that the body weight and spleen weight of mice was not influenced by Qiweibaizhusan and yeast. Weight of thymus was promoted by Qiweibaizhusan and yeast (P > 0.05). Ultra-micro Qiweibaizhusan and yeast could regulate the microecological balance by promoting the growth of Bifidobacterium and Lactobacillus (P < 0.01). The Escherichia coli and total bacteria amount of the 50% amount ultra-micro Qiweibaizhusan group and the 25% amount ultra-micro decoction + 25% yeast group recovered to the normal level (P > 0.05). After treatment, the intestinal cellulase enzyme activity recovered to the normal group level (P > 0.05). The amylase activity of the 50% amount ultra-micro Qiweibaizhusan + 12.5% yeast group and the 25% amount ultra-micro Qiweibaizhusan + 25% yeast group recovered to the normal (P > 0.05). The activity of xylanase and protease had a certain relationship with the concentration of ultra-micro Qiweibaizhusane and yeast, but did not recover to the normal level (P < 0.05). Among all ratios, 25% amount ultra-micro Qiweibaizhusan + 25% yeast had the best compatibility and the same effect on dysbacteriosis diarrhea with 50% amount ultra-micro Qiweibaizhusan and 100% amount traditional Qiweibaizhusan.

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备注/Memo

备注/Memo:
国家自然科学基金项目(81173214)资助 Supported by the National Natural Science Foundation of China (81173214)
更新日期/Last Update: 2015-02-15