|本期目录/Table of Contents|

[1]郭倩,张铁雨,冯进辉,等.高效转化胆固醇菌株的筛选、鉴定及转化条件优化[J].应用与环境生物学报,2014,20(01):101-106.[doi:10.3724/SP.J.1145.2014.00101]
 GUO Qian,ZHANG Tieyu,FENG Jinhui,et al.Screening, identification and condition optimization of a bacterial strain efficient in biotransforming cholesterol[J].Chinese Journal of Applied & Environmental Biology,2014,20(01):101-106.[doi:10.3724/SP.J.1145.2014.00101]
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高效转化胆固醇菌株的筛选、鉴定及转化条件优化()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年01期
页码:
101-106
栏目:
研究论文
出版日期:
2014-02-25

文章信息/Info

Title:
Screening, identification and condition optimization of a bacterial strain efficient in biotransforming cholesterol
作者:
郭倩张铁雨冯进辉王玉陈曦路福平吴洽庆朱敦明
1天津科技大学生物工程学院 天津 300457 2中国科学院天津工业生物技术研究所,天津市生物催化技术工程中心 天津 300308 3浙江省食品药品检验研究院 浙江 310004
Author(s):
GUO Qian ZHANG Tieyu FENG Jinhui WANG Yu CHEN Xi LU Fuping WU Qiaqing ZHU Dunming
1 College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China 2 Tianjin Engineering Center for Biocatalytic Technology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China 3 Zhejiang Institute for Food and Drug Control, Hangzhou 310004, China
关键词:
胆固醇4-胆甾烯-3-酮胆固醇氧化酶红球菌植物甾醇
Keywords:
cholesterol 4-cholesten-3-one cholesterol oxidase Rhodococcus phytosterol
分类号:
Q939.9 : TQ464
DOI:
10.3724/SP.J.1145.2014.00101
文献标志码:
A
摘要:
胆固醇由甾体母核和侧链构成,是一种新的甾体资源. 以胆固醇为唯一碳源的选择培养基,利用富集培养法从土壤中筛选出一株细菌,编号5901. 该菌能够快速高效转化胆固醇,专一性生成4-胆甾烯-3-酮. 经形态学观察和16S rDNA序列比对分析,该菌株为Rhodococcus sp. 该菌株转化胆固醇的最优条件为:生长温度和转化温度均为30 ℃,初始pH 8.0. 胆固醇质量浓度为5 g/L,反应24 h后,胆固醇转化率为95%. 本研究表明,5901菌株较之前报道的菌株,底物浓度高,转化速率快,也可以催化其它甾体化合物的3β-羟基氧化和5位双键位移反应,具有潜在的研究和应用价值.
Abstract:
Cholesterol is a new kind of steroidal resources, which is composed of steroidal mother nucleus and side chain. A new strain 5901, which effectively converted cholesterol to 4-cholesten-3-one, was isolated from soil samples and identified as Rhodococcus sp. by the morphological observation and 16S rDNA gene sequence analysis. The optimal condition for the strain to convert cholesterol was 30 °C for growth and transformation, and 8.0 for initial pH. The conversion of cholesterol reached 95% under the optimal condition after 24 h when the substrate concentration was 5 g/L. From practical point of view, this strain has relatively higher substrate tolerance and transformation rate than the earlier reported strains. In addition, it also oxidizes 3b-hydroxyl group and ?5→4 isomerization of other steroids. These results suggest that it may be a useful strain for potential industrial application.

参考文献/References:

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备注/Memo

备注/Memo:
国家高技术研究发展计划(“863”计划)项目(2011AA02A211)和天津市科技支撑计划项目(10ZCZDSY06700)资助
更新日期/Last Update: 2014-03-06