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[1]朱启彧,安君霞,李甫,等.Curcusone C诱导卵巢癌SK-OV-3细胞凋亡[J].应用与环境生物学报,2013,19(06):956-959.[doi:10.3724/SP.J.1145.2013.00956]
　ZHU Qiyu,AN Junxia,LI Fu,et al.Curcusone C Induces Apoptosis in Human Ovarian Cancer SK-OV-3 Cells[J].Chinese Journal of Applied & Environmental Biology,2013,19(06):956-959.[doi:10.3724/SP.J.1145.2013.00956]
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Curcusone C诱导卵巢癌SK-OV-3细胞凋亡()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年06期
页码:
956-959
栏目:
研究论文
出版日期:
2013-12-25

文章信息/Info

Title:
Curcusone C Induces Apoptosis in Human Ovarian Cancer SK-OV-3 Cells
作者:
朱启彧; 安君霞; 李甫; 王明奎; 吴坚; 唐亚雄
（1西南交通大学生命科学与工程学院 成都 610031） （2中国科学院成都生物研究所 成都 610041）
Author(s):
ZHU Qiyu; AN Junxia; LI Fu; WANG Mingkui; WU Jian; TANG Yaxiong
(1School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China) (2Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
关键词:
Curcusone C; 卵巢癌细胞SK-OV-3; 细胞增殖; 细胞凋亡; PARP; 麻风树
Keywords:
Curcusone C;  SK-OV-3;  proliferation;  apoptosis;  PARP;  Jatropha curcas
分类号:
Q949.753.506 + R966
DOI:
10.3724/SP.J.1145.2013.00956
文献标志码:
A
摘要:
近年来麻风树（Jatropha curcas）的生物活性研究如火如荼，但其二萜类化合物的抗癌活性及分子机制研究仍知之甚少. 通过萃取和柱层析方法从麻风树的根皮中分离提取到一种二萜类化合物Curcusone C，探讨了该化合物对卵巢癌SK-OV-3细胞增殖、细胞凋亡的影响. 实验结果表明，Curcusone C能显著抑制SK-OV-3细胞的增殖，且随着药物浓度的增加其抑制作用呈明显的剂量依赖效应，其48 h半数抑制浓度IC50为160 nmol/L；同时，Curcusone C能显著诱导SK-OV-3细胞凋亡，DAPI染色结合显微镜观察发现400 nmol/L Curcusone C处理后的细胞呈现典型的细胞凋亡形态学特征；最后，Western blot免疫印迹表明Curcusone C能显著诱导SK-OV-3细胞的聚ADP核糖聚合酶（PARP）经Caspase切割为Mr 89×103降解产物，从而从分子水平上证实Curcusone C能以剂量依赖方式激活PARP的切割活性进而促进细胞凋亡. 上述结果表明从麻风树根皮中提取的二萜类化合物Curcusone C对人卵巢癌的预防与治疗可能具有潜在价值.
Abstract:
Curcusone C, as a diterpenoid compound, has been isolated and identified by solvent extraction and column chromatography from Jatropha curcas. This paper investigated the anti-cancer potential of Curcusone C on human ovarian cancer SK-OV-3 cells. The results in vitro demonstrated that Curcusone C significantly suppressed proliferation of SK-OV-3 cells in dose-dependent manner, with the IC50 value as 160 nmol/L under treatment with Curcusone C for 48 h. Curcusone C also markedly induced the apoptosis of SK-OV-3 cells, which showed typical apoptotic features including blebbing at the membranes and condensed chromatin with DAPI staining. In addition, Western blot clearly confirmed that the apoptotic effect was associated with activation of the cleavage of PARP, leading to the inhibitory effect of SK-OV-3 cells. All together, these findings suggest that Curcusone C may be a promising antitumor lead compound for prevention and treatment of ovarian cancer.

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备注/Memo

备注/Memo:
国家自然科学基金项目（81173095）和四川省国际合作计划（2013HH0014）资助
更新日期/Last Update: 2014-01-03