|本期目录/Table of Contents|

[1]罗聪佩,何涛,淳泽.红外光谱法在石斛鉴别中的应用研究进展[J].应用与环境生物学报,2013,19(03):537-541.[doi:10.3724/SP.J.1145.2013.00537]
 LUO Congpei,HE Tao,CHUN Ze.Advances in Infrared Spectroscopy Methods for Discriminating Dendrobium[J].Chinese Journal of Applied & Environmental Biology,2013,19(03):537-541.[doi:10.3724/SP.J.1145.2013.00537]
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红外光谱法在石斛鉴别中的应用研究进展()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年03期
页码:
537-541
栏目:
综述
出版日期:
2013-06-25

文章信息/Info

Title:
Advances in Infrared Spectroscopy Methods for Discriminating Dendrobium
作者:
罗聪佩何涛淳泽
(1中国科学院成都生物研究所 成都 610041)
(2中国科学院大学 北京 100049)
Author(s):
LUO CongpeiHE TaoCHUN Ze
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
(2University of Chinese Academy of Sciences, Beijing 100049, China)
关键词:
石斛生药鉴别红外光谱化学计量学中药
Keywords:
Dendrobium discrimination infrared spectroscopy chemometrics traditional Chinese medicine
分类号:
R282.5 : Q949.718.43
DOI:
10.3724/SP.J.1145.2013.00537
摘要:
名贵中药石斛原植物来源属于珍稀濒危植物,是多基源(中国记载分布有76种)、同部位入药的中药材,为中国药典中基源收载最为复杂的中药之一,需要简便、快速的方法来对不同基源的石斛进行鉴别. 红外光谱法具有快速简便、指纹性强等特点,特别适合中药材这一复杂体系的快速鉴别. 本文简要介绍了红外光谱及红外光谱法鉴别中药的原理以及与红外光谱法鉴别中药相关的化学计量学方法的基本原理和特点;综述了近年来红外光谱及其二阶导数谱对比法、二维相关红外光谱法、红外光谱结合化学计量学方法在石斛属植物鉴别中的应用研究进展,研究表明红外光谱鉴别石斛具有简便快速、特征性强、取样量少等优点. 随着红外光谱技术本身的发展及其在石斛研究中的广泛应用,该方法将成为石斛鉴别的主要方法之一.
Abstract:
Dendrobium, as a precious Chinese medicine, is a rare and endangered plant. Because the same part of the plant of multiple sources is used for medical purposes, Dendrobium is one of the most complicated entries recorded in Chinese Pharmacopoeia. Therefore, a convenient and fast method is needed to discriminate different species of Dendrobium. Infrared spectroscopy is fast, convenient, and highly sensitive with fingerprint, thus extremely suitable for fast discriminating the complex system of traditional Chinese medicines. This paper introduces infrared spectroscopy and its application in discriminating traditional Chinese medicines, as well as the basic principles and characteristics of infrared spectroscopy-related Chemometrics. We examined the application progresses in discriminating Dendrobium of infrared spectroscopy and derivative methods, including two-dimensional infrared spectroscopy method and infrared spectroscopy-related Chemometrics method, showing that infrared spectroscopy has the advantages of convenience, efficiency, fingerprint and little-dosage in discriminating Dendrobium. With the development of infrared spectroscopy and its extensive use in research on Dendrobium, this technology will become one of the main discriminating methods for Dendrobium discrimination.

参考文献/References:

1 何涛, 淳泽, 罗傲雪, 范益军, 刘静, 胡明珠. 四川石斛野生资源及其保护研究[J]. 应用与环境生物学报, 2008, 14 (5): 710-715 [He T, Chun Z, Luo AX, Fan YJ, Liu J, Hu MZ. Wild resources and conservation of Dendrobium in Sichuan, China [J]. Chin J Appl Environ Biol, 2008, 14 (5): 710-715]
2 林萍, 毕志明, 徐红, 王峥涛, 徐珞珊. 石斛属植物药理活性研究进展[J]. 中草药, 2003, 34 (11): 19-21 [Lin P, Bi ZM, Xu H, Wang ZT, Xu LS. Advances in studies on pharmacology of plants from Dendrobium Sw [J]. Chin Trad Herbal Drugs, 2003, 34 (11): 19-21]
3 国家药典委员会. 中国药典[M]. 北京: 中国医药科技出版社, 2010 [Chinese Pharmacopoeia Commission. Chinese Pharmacopoeia [M]. Beijing: China Medical Science Press, 2010]
4 陈亚, 江滨, 曾元儿. 红外光谱在中药鉴别中的应用[J]. 广州中医药大学学报, 2004, 21 (3): 237-240
5 姜大成, 何淑华, 张洁. 红外光谱鉴定中药材的原理与方法[J]. 中药材, 1993, 16 (7): 42-43
6 Lei Y, Zhou Q, Ya T, Chen JB, Sun SQ, Noda I. The study of Cistanche deserticola using Fourier transform infrared spectroscopy combined with two-dimensional correlation infrared spectroscopy [J]. J Mol Struct, 2010, 974: 156-160
7 Noda I. Two-dimensional infrared spectroscopy [J]. J Am Chem Soc, 1989, 111: 8116-8118
8 孙素琴, 周群, 秦竹. 中药二维相关红外光谱鉴定图集[J]. 北京: 化学工业出版社, 2003 [Sun SQ, Zhou Q, Qin Z. Atlas of two-dimensional correlation infrared spectroscopy for traditional Chinese medicine identification [J]. Beijing: Chemical Industry Press, 2003]
9 Wold S. Chemometrics; what do we mean with it, and what do we want from it [J]? Chemom Intell Lab Syst, 1995, 30 (1995): 109-115
10 Pearson K. On lines and planes of closest fit to systems of points in space [J]. Philos Mag, 1901, 2 (6): 559-572
11 LU HF, Cheng CG, Tang X, Hu ZH. FTIR spectrum of Hypericum and Triadenum with reference to their identification [J]. Acta Bot Sin, 2004, 46 (4): 401-406
12 邹华彬, 袁久荣, 杜爱琴, 孙琳琳, 秦正冉. 甘草水提物红外指纹图谱共有峰率和变异峰率双指标序列分析法[J]. 中成药, 2004, 26 (10): 779-783 [Zou HB, Yuan JR, Du AQ, Sun LL, Qin ZR. Common and variant peak ratio in IR fingerprint of extraction from Radix Glycyrrhizae by dual-index sequence analysis [J]. Chin Trad Patent Med, 2004, 26 (10): 779-783]
13 赵同芳, 王宪楷. 不同树生和石生金钗石斛成分的光谱和层析谱的研究[J]. 华西医科大学学报, 1986, 17 (4): 332-335 [Zhao TF, Wang XK. Studies on the application of spectra and thin-layer chromatography to the constituents of Dendrobium nobile cultivated on various trees and stone [J]. J West China Univ Med Sci, 1986, 17 (4): 332-335]
14 华文俊, 周洪雷, 孙勇. 石斛、天竺黄、牛蒡子与伪品的红外光谱比较[J]. 中医药研究, 2000, 16 (2): 53-54
15 邓月娥, 孙素琴, 牛立元. 不同产地金钗石斛红外光谱解析[J]. 河南科技学院学报, 2007, 35 (2): 38-40 [Deng YE, Sun SQ, Niu LY. Infrared spectrum features of Dendrobium nobile Lindl from different growth places [J]. J Henan Inst Sci Technol, 2007, 35 (2): 38-40]
16 李兆奎, 孙彩华, 李美琴. 铁皮石斛与几种常用混淆品的红外光谱鉴别[J]. 海峡药学, 2005, 17 (3): 91-93 [LI ZK, Sun CH, Li MQ. IR identification of D. candidum Wall ex Lindl from some common confused articles [J]. Strait Pharm J, 2005, 17 (3): 91-93]
17 陈祝霞, 时雅旻. 石斛类药材的红外光谱鉴别研究及其抗氧化活性比较[J]. 中国现代中药, 2007, 9 (8): 22-27 [Chen ZX, Shi YM. Studies on the IR spectra and antioxidative activities of 12 species plant from caulis Dendrobii [J]. Mod Chin Med, 2007, 9 (8): 22-27]
18 刘宁. 金钗石斛质量控制方法研究[R]. 北京: 北京中医药大学, 2009. 69-74
19 Wang CY, Xiang BR, Zhang W. Application of two-dimensional near-infrared (2D-NIR) correlation spectroscopy to the discrimination of three species of Dendrobium [J]. J Chemom, 2009, 23: 463-470
20 白音. 药用石斛鉴别方法的系统研究[R]. 北京: 北京中医药大学, 2007. 69-81
21 吕献康, 程存归, 杨国平, 金勇, 叶涵, 徐东尉. 11种石斛植物的FTIR直接测定和主成分分析[J]. 中国中药杂志, 2005, 30 (10): 738-740 [Lü XK, Cheng CG, Yang GP, Jin Y, Ye H, Xu DW. Application of FTIR spectroscopy to the analysis of eleven kinds of Dendrobium [J]. China J Chin Mat Med, 2005, 30 (10): 738-740]
22 沈宗根, 吕洪飞, 程存归. 3种石斛属植物和细叶石仙桃的红外光谱分析和鉴定[J]. 西北植物学报, 2008, 28 (1): 97-102 [Shen ZG, Lü HF, Cheng CG. FTIR identification and analysis of three species in Dendrobium and Pholidata cantonensis [J]. Acta Bot Bor-Occid Sin, 2008, 28 (1): 97-102]
23 李莹, 孙卓然, 刘圆, 刘超. 不同种石斛的红外指纹图谱研究[J]. 西南民族大学学报(自然科学版), 2009, 35 (5): 1024-1027 [李莹, 孙卓然, 刘圆, 刘超. Fingerprint analysis on different species of herba Dendrobii by IR [J]. J Southwest Univ for Nationalities (Nat Sci Ed), 2009, 35 (5): 1024-1027]
24 赵前程, 王晓兰, 武中臣, 关洪斌. FTIR在中药研究中的应用及优化[J]. 时珍国医国药, 2011, 22 (6): 1466-1468 [Zhao QC, Wang XL, Wu ZC, Guan HB. Research of traditional Chinese medicine by Fourier transform infrared spectroscopy and its optimization [J]. LISHIZHEN Med Mat Med Res, 2011, 22 (6): 1466-1468]
25 李燕, 吴然然, 于佰华, 王俊德. 红外光谱在中药定性定量分析中的应用[J]. 光谱学与光谱分析, 2006, 26 (10): 1846-1849 [Li Y, Wu RR, Yu BH, Wang JD. A review on applications of infrared spectroscopy to the study of traditional Chinese medicine [J]. Spectrosc Spectral Anal, 2006, 26 (10): 1846-1849]
26 王一兵, 吴卫红, 陈植成, 覃国乐, 姚辉璐, 黄庶识. 傅里叶变换红外光谱法和紫外-可见谱线组法分析广西特产罗汉果[J]. 光谱实验室, 2009, 24 (4): 907-911 [Wang YB, Wu WH, Chen ZC, Qin GL, Yao HL, Huang SS. Qualitative analysis of dry-fruit of Siraitia Grosvenorii by FT-IR and UV-Vis spectrometry [J]. Chin J Spectrosc Lab, 2009, 24 (4): 907-911]

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备注/Memo

备注/Memo:
中国科学院知识创新工程重要方向项目和院地合作项目(XBCD-2011-007)、国家科技支撑计划项目(2011BAC09B04)、国家及四川省农业科技成果转化资金项目(2011GB2F000004)、四川省“十二五”中药材育种攻关项目(2011NZ0098-12-10)、四川省科技支撑计划项目(2010NZ0057)和四川省科技条件平台建设项目资助
更新日期/Last Update: 2013-06-20