|本期目录/Table of Contents|

[1]王兴彪,张晴,刘洋,等.脱水原油中的微生物及其原油降解活性[J].应用与环境生物学报,2013,19(03):515-518.[doi:10.3724/SP.J.1145.2013.00515]
 WANG Xingbiao,ZHANG Qing,LIU Yang,et al.Microorganisms and Their Oil-degrading Activities in Dehydrated Crude Oil[J].Chinese Journal of Applied & Environmental Biology,2013,19(03):515-518.[doi:10.3724/SP.J.1145.2013.00515]
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脱水原油中的微生物及其原油降解活性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年03期
页码:
515-518
栏目:
研究论文
出版日期:
2013-06-25

文章信息/Info

Title:
Microorganisms and Their Oil-degrading Activities in Dehydrated Crude Oil
作者:
王兴彪张晴刘洋汤岳琴吴钢吴晓磊
(1中国科学院生态环境研究中心 北京 100085)
(2北京大学工学院 北京 100871)
(3中国科学院大学 北京 100049)
Author(s):
WANG XingbiaoZHANG QingLIU YangTANG YueqinWU GangWU Xiaolei
(1Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China)
(2College of Engineering, Peking University, Beijing 100871, China)
(3University of Chinese Academy of Sciences, Beijing 100049, China)
关键词:
原油细菌群落分散乳化活性原油微生物原油降解活性大庆油田
Keywords:
crude oil bacterial community oil-dispersing and oil-emulsifying activity microorganism in crude oil oil-degrading activity Daqing Oilfield
分类号:
Q938.15 : X74
DOI:
10.3724/SP.J.1145.2013.00515
摘要:
为研究大庆油田脱水原油中的微生物群落结构、单菌及其降解原油的活性,系统分析了大庆油田脱水原油中的微生物分布及细胞活性. 结果表明,大庆油田脱水原油中存在活体微生物细胞,进一步筛选到一个具有乳化分散活性的微生物群落,群落结构分析证明群落中的微生物种类和比例随着培养时间延长不断变化. 从原油培养体系中分离纯化到166株细菌,活性测试结果表明,具有分散或乳化活性的菌株比例占测试菌株总数的13.1%. 研究结果表明,大庆油田油藏中存在对原油具有降解乳化分散活性的微生物群落,恶劣的地层环境和营养的缺乏导致群落密度较小,但降解原油的活性菌株比例较高.
Abstract:
This study analyzed the microbial community structure, microorganisms and the oil-degrading activities in the dehydrated crude oil from Daqing Oilfield. The results showed the existence of many microbial cells in the dehydrated crude oil, and screened a microbial community with oil-emulsifying and oil-dispersing activity. Community analysis showed that the types and rations of the microorganisms in the community changed with incubation time. All together 166 bacteria were isolated and purified from the crude oil culture system, up to 13.1% of the tested strains having oil-dispersing or oil-emulsifying activities. The results showed that there are microbial communities with oil emulsifying and oil dispersing activities in Daqing oil reservoir, that the harsh conditions and the lack of nutrition leads to smaller community density, and that the oil degrading strains occupy a high proportion.

参考文献/References:

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备注/Memo

备注/Memo:
国家自然科学基金项目(31070107)资助
更新日期/Last Update: 2013-06-20