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[1]谭周进,张华玲,周赛男,等.菌群失调小鼠腹泻造模及超微中药干预过程中肠道微生物的变化[J].应用与环境生物学报,2013,19(03):449-453.[doi:10.3724/SP.J.1145.2013.00449]
　TAN Zhoujin,ZHANG Hualing,ZHOU Sainan,et al.Change of Intestinal Microbes in Dysbacteriosis-modeled Mice Treated with Ultra-micro Powder of Qiweibaizhusan[J].Chinese Journal of Applied & Environmental Biology,2013,19(03):449-453.[doi:10.3724/SP.J.1145.2013.00449]
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菌群失调小鼠腹泻造模及超微中药干预过程中肠道微生物的变化()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年03期
页码:
449-453
栏目:
研究论文
出版日期:
2013-06-25

文章信息/Info

Title:
Change of Intestinal Microbes in Dysbacteriosis-modeled Mice Treated with Ultra-micro Powder of Qiweibaizhusan
作者:
谭周进; 张华玲; 周赛男; 余望贻; 曾奥; 蔡莹; 蔡光先
（1湖南中医药大学 长沙 410208）
（2湖南中医药大学 2010级研究生班 长沙 410208）
Author(s):
TAN Zhoujin; ZHANG Hualing; ZHOU Sainan; YU Wangyi; ZENG Ao; CAI Ying; CAI Guangxian
(1Hunan University of Chinese Medicine, Changsha 410208, China)
(22010 Postgraduate Courses Class, Hunan University of Chinese Medicine, Changsha 410208, China)
关键词:
超微中药; 七味白术散; 菌群失调; 肠道微生物; 中医药微生态
Keywords:
ultra-micro powder of traditional Chinese medicine;  Qiweibaizhusan powder;  dysbacteriosis;  intestinal microbe;  microecology of traditional Chinese medicine
分类号:
R285.5
DOI:
10.3724/SP.J.1145.2013.00449
摘要:
采用灌胃头孢拉定和硫酸庆大霉素混合抗生素进行小鼠菌群失调腹泻造模，灌胃给药1/2量超微七味白术散治疗，正常组和模型组灌胃等量的无菌水，在不同时间段取肠道内容物分析5类可培养微生物的数量，研究其动态变化. 结果显示造模过程中，细菌总数、大肠杆菌数都是先急剧减少，后稍有恢复，但仍低于初始水平，乳酸菌数先急剧减少后又显著增加，高于正常组水平，酵母菌和霉菌数则呈现持续增加的趋势；经1/2量超微七味白术散治疗后，细菌总数持续增加，大肠杆菌数呈先增后减的趋势，乳酸菌和酵母菌数则先增加，治疗4 d后趋于稳定，霉菌则持续减少. 研究表明1/2量超微七味白术散对菌群失调小鼠肠道菌群具有明显的调整作用，有效缩短了肠道菌群由失调到平衡的时间.
Abstract:
This experiment aimed to study the change of intestinal microbes with dysbacteriosis treated by ultra-micro powder of Qiweibaizhusan. The mice were modeled dysbacteriosis by stomach injection of mixed cefradine and gentamycin sulfate. The treatment group received intragastric administration of half-dose ultra-micro Qiweibaizhusan, and the normal group mice and model group mice were given steriled water. Selective media were used to culture five kinds of gut microbes in different treatment times. The results showed that during modeling period, the number of bacteria and colibacillus had decreased rapidly and partly recovered, but still lower than the initial level. The number of lactobacillus increased significantly after a sharp decrease, higher than normal level. The number of yeast and mold presented an increasing trend. During the treatment period with half-dose ultra-micro Qiweibaizhusan, the total bacteria number increased all the time, and the number of Escherichia coli presented a decreasing trend after a slight increase. The number of lactobacillus and yeast increased first, and became stable after four days of treatment. But the number of mold decreased all the time. The results showed that the half-dose ultra-micro powder of Qiweibaizhusan could effectively regulate and improve intestinal micro-flora with dysbacteriosis in mice, and shorten the time from disturbance to balance.

参考文献/References:

1 郭维军. 七味白术散加减治疗肠易激综合征脾胃虚弱证的临床观察[J]. 湖南中医药大学学报, 2010, 30 (9): 175-177 [Duo WJ. Effect of modified Qiweibaizhu Powder on irritable bowel syndrome with weakened spleen and stomach [J]. J Trad Chin Med Univ Hunan, 2010, 30 (9): 175-177]
2 蔡光先, 杨永华. 中药饮片改革的新探索——单味中药超微饮片[J]. 湖南中医杂志, 2001, 17 (6): 50 [Cai GX, Yang YH. New approaches for reform of Chinese herbal medicine - single herb ultramicro [J]. Hunan J Trad Chin Med, 2001, 17 (6): 50]
3 谷丽丽, 张雪梅, 田莉瑛, 边艳青, 赵宝华. 中药超微粉碎技术的应用及进展[J]. 生命科学仪器, 2008, 6: 49-52 [Gu LL, Zhang XM, Tian LY, Bian YQ, Zhao BH. Applications and progress Chinese medicine ultra-fine powder technology[J]. Life Sci Instrum, 2008, 6: 49-52]
4 邓红洁, 吴海, 彭晓珊, 蔡光先, 张熙, 谭周进, 欧阳锋. 超微七味白术散对菌群失调小鼠的疗效研究[J]. 现代生物医学进展, 2011, 11 (19): 3632-3635 [Deng HJ, Wu H, Peng XS, Cai GX, Zhang X, Tan ZJ, Ouyang F. Effects of ultra-micro powder of Qiweibaizhu on dysbacteriosis in mice with diarrhea [J]. Progr Mod Biomed, 2011, 11 (19): 3632-3635]
5 Backhed F, Ley RE, Sonnenburg JL, Peterson DA, Gordon JI. Host-bacterial mutualism in the human intestine [J]. Science, 2005, 307: 1915-1920
6 Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L, Sargent M, Gill SR, Nelson KE, Relman DA. Diversity of the human intestinal microbial flora [J]. Science, 2005, 308: 1635-1638
7 Round JL, Mazmanian SK. The gut microbiota shapes intestinal immune responses during health and disease[J]. Nat Rev Immunol, 2009, 9 (5): 313-323
8 李刚. 七味白术散加减治疗小儿霉菌性肠炎48例[J]. 陕西中医, 2009, 30 (3): 322-323 [Li G. Qiweibaizhu Powder for the treatment of children with fungal enteritis 48 cases[J]. Shaanxi J Trad Chin, 2009, 30 (3): 322-323]
9 刘双英. 七味白术散治疗小儿脾虚湿盛型腹泻120例[J]. 陕西中医, 2009, 30 (10): 1331-1332 [Liu SY. Qiweibaizhu Powder treatment of pediatric spleen dampness type diarrhea 120 cases [J]. Shaanxi J Trad Chin, 2009, 30 (10): 1331-1332]
10 杨波, 伍参荣, 唐小梅, 胡建中, 赵武能. 七味白术散对人轮状病毒感染乳鼠血清IFN-γ、TNF-α的影响[J]. 中国中医药信息杂志, 2005, 12 (3): 36-37 [Yang B, Wu CR, Tang XM, Hu JZ, Zhao WN. Influence of Qiweibaizhu Powder on the serum IFN- γ and TNF-α levels of suckling mice infected with human rotavirus (HRV) [J]. Chin J Inform Trad Chin Med, 2005, 12 (3): 36-37]
11 王晓强. 七味白术散对脾虚泄泻幼鼠免疫机能的影响[D]. 哈尔滨: 黑龙江中医药大学, 2007 [Wang XQ. Qiweibaizhu Powder influence on the immune function of the spleen diarrhea in young rats [D]. Harbin: Heilongjiang University Of Chinese Medicine, 2007]
12 沈萍, 陈向东. 微生物学[M]. Beijing: Higher Education Publishing House, 2008 [Shen P, Chen XD. Microbiology Experiment [M]. Beijing: Higher Education Publishing House, 2008]
13 凌代文. 乳酸细菌分类鉴定及实验方法[M]. Beijing: 中国轻工业出版社, 1999 [Ling DW. The Experimental Method of Classification and Identification for Lactobacillus [M]. Beijing: China Light Industry Publishing House, 1999]
14 曾奥, 张华玲, 谭周进, 蔡莹, 蔡光先, 吴海. 小鼠菌群失调腹泻模型的建立及超微七味白术散的疗效[J]. 微生物学通报, 2012, 39 (9): 1341-1348 [Zeng A, Zhang HL, Tan ZJ, Cai Y, Cai GX, Wu H. The construction of mice diarrhea model due to dysbacteriosis and curative effect of ultra-micro Qiweibaizhusan[J]. Microbiology, 2012, 39, (9): 1341-1348]
15 谭周进, 吴海, 刘富林, 蔡莹, 蔡光先, 张华玲, 曾奥. 超微七味白术散对肠道微生物及酶活性的影响[J]. 生态学报, 2012, 32 (21): 6856-6863 [Tan ZJ, Wu H, Liu FL, Cai Y, Cai GX, Zhang HL, Zeng A. Effect of ultra-micro powder Qiweibaishusan on the intestinal microorganisms and enzyme activities [J]. J Ecol, 2012, 32 (21): 6856-6863]
16 梁冰, 吴力克, 房芳, 姜峰, 詹瑛, 张学成. 螺旋藻制剂调节实验性肠菌群失调症药效学研究[J].中国海洋药物, 2002, 1: 29-33 [Liang B, Wu LK, Fang F, Jiang F, Zhan Y, Zhang XC. Determination of pharmacodynamics of spirulina on the experimental imbalance of mouse intestinal bacterial flora [J]. Chin J Mar Drugs, 2002, 1: 29-33]
17 宋金春, 蔡华, 谢腾芳, 马占军. 抗微生物药物学[M]. 北京: 科学出版社, 2010 [Song JC, Cai H, Xie TF, MA ZJ. Antimicrobial Drugs [M]. Beijing: Science Press, 2010]
18 姜良铎, 赵长琦. 中药学和微生态学[M]. 北京: 化学工业出版社, 2008 [Jiang LD, Zhao CQ. Traditional Chinese medicine and micro-ecology[M]. Beijing: Chemical Industry Publishing House, 2008]
19 张凤普. 人参皂苷的作用机理研究[J]. 临床合理用药杂志, 2010, 3 (21): 158
20 陈晓萍, 张长林. 白术不同化学成分的药理作用研究概况[J]. 中医药信息, 2011, 28 (2): 124-126 [Chen XP, Zhang CL. Pharmacological effects profiles of different chemical composition of Atractylodes [J]. Inform Trad Chin, 2011, 28 (2): 124-126]
21 徐轶尔, 李秋红, 杨菲菲. 中药葛根的药理药效研究[J]. 吉林中医药, 2010, 30 (11): 993-995 [Xu YE, Li QH, Yang FF. The pharmacological effect of the Radix Puerariae [J]. Jilin J Trad Chin, 2010, 30 (11): 993-995]
22 Haraguchi H, Tanimoto K, Tanmura Y, izutani K, Kinoshita T. Mode of antibacterialaction of retrochalcones from Glycyrrhiza inflata [J]. Phytochemistry, 1998, 48 (1): 125-129
23 朱婷, 蔡光先, 吴海, 文丹, 谭周进. 植物成分对肠道微生物的影响[J]. 中国药业 , 2010, 19 (18): 19-21 [Zhu T, Cai GX, Wu H, Wen D, Tan ZJ. Impacts of plant components to intestinal tract microflora [J]. China Pharm, 2010, 19 (18): 19-21]
24 小桥恭一. 中药活性成分与肠道微生物的关系[J]. 中医药理论研究, 1995, 16 (11): 598 [Kyoichi K. The relationship between active ingredients of Chinese medicine and intestinal bacteria [J]. Theory Chin Med, 1995, 16 (11): 598]

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备注/Memo

备注/Memo:
国家自然科学基金项目（81173214）、湖南省科技厅重点项目（2010SK2002）和湖南省科技厅一般项目（2011RS4018）资助
更新日期/Last Update: 2013-06-20