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 REN Haiwei,LI Jinping,ZHANG Yi,et al.Optimization of Saccharification for Distillers Grains and Ethanol Fermentation[J].Chinese Journal of Applied & Environmental Biology,2013,19(05):833-837.[doi:10.3724/SP.J.1145.2013.00833]





Optimization of Saccharification for Distillers Grains and Ethanol Fermentation
(1兰州理工大学西部能源与环境研究中心 兰州 730050) (2四川大学生命科学学院 成都 610041) (3中国科学院大学 北京 100049)
REN Haiwei LI Jinping ZHANG Yi WANG Yonggang CHEN Xiaoqian LI Zhizhong
(1Western China Energy & Environment Research Center, Lanzhou University of Technology, Lanzhou 730050, China) (2School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China) (3School of Energy and Power Engineering, Lanzhou University of Technology, Lanzhou 730050, China)
distiller grains acid hydrolysis saccharification reducing sugar furfural ?scanning electron microscopy (SEM) infrared spectroscopy (FTIR) ethanol fermentation
为探索白酒丢糟水解糖化的最优工艺及乙醇发酵可行性,以还原糖浓度和糠醛生成量为指标,考察温度、固液比、混合酸浓度和时间4个因素对混合酸水解糖化丢糟效果的影响,利用正交试验优化糖化条件,采用扫描电镜(SEM)和红外光谱(FTIR)技术研究丢糟水解前后的结构特性,并利用最优条件获得的丢糟水解液发酵制备乙醇. 结果表明,最优的丢糟糖化条件为温度100 ℃,固液比1:12,混合酸浓度2.5%,水解时间120 min,该条件下水解液中的还原糖浓度为59.32 g/L,糠醛生成量为8.37 g/L. SEM结果显示,酸解糖化后的丢糟结构发生了明显变化,木质纤维结构被破坏,基本形貌完全改变;FTIR证明丢糟中纤维素和半纤维素成分被部分降解,水解糖化效果明显. 酿酒酵母(Saccharomyces cerevisiae)发酵丢糟水解液72 h后的乙醇浓度和产率分别为11.82 g/L和0.14 g/g,还原糖利用率为80.47%. 说明丢糟经水解糖化后发酵乙醇是可行的. 图4 表3 参33
Distiller抯 grains (DG) is an abundant source of biomass that can be hydrolyzed to yield fermentation sugar for t?he production of fuel ethanol. In order to improve the saccharification efficiency and investigate the hydrolysis characteristics of DG, this research studied the effect of temperature, mixed acid concentration, the solid-liquid ratio and hydrolysis time on the concentration of reducing sugar and the yield quantity of furfural. In addition, the structure of DG and acid hydrolysis residue were characterized by methods of SEM and FTIR. DG saccharification liquid was fermented by Saccharomyces cerevisiae to produce ethanol. The results of the orthogonal test and range analysis of experiment showed the optimal levels for saccharification as temperature of 100 ℃, solid-liquid ratio of 1:12, mixed acid concentration of 2.5% and hydrolysis time of 120 min. The concentration of reducing sugar and the yield quantity of furfural was 59.32 g/L and 8.37 g/L respectively under optimized conditions. SEM analysis show that the structure of DG after hydrolyzing was destroyed remarkably, lignocellulosic structure destroyed, and the basic morphology changed totally. The FTIR results proved that the composition of cellulose and hemicellulose were partially hydrolyzed, with lignin removed. Accordingly, the concentration of ethanol was up to 11.82 g/L, with the yield 0.14 g/g and utilization of reducing sugar 80.47% after 72h. In conclusion, it is feasible to produce ethanol by fermenting saccharification liquid from DG. Fig 4, Tab 3, Ref 33


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[1]孙彦平,靳艳玲,郜晓峰,等.纤维素酸解副产物对Clostridium acetobutylicum CICC8012发酵的影响[J].应用与环境生物学报,2010,16(06):845.[doi:10.3724/SP.J.1145.2010.00845]
 SUN Yanping,JIN Yanling,GAO Xiaofeng,et al.Effects of Byproducts from Acid Hydrolysis of Lignocellulose on Butanol Fermentation by Clostridium acetobutylicum CICC8012[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):845.[doi:10.3724/SP.J.1145.2010.00845]


收稿日期 Received: 2012-09-03 接受日期 Accepted: 2013-06-28
更新日期/Last Update: 2013-10-28