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[1]陆辰霞,刘龙,李江华,等.淀粉填充聚乙烯类塑料降解微生物的筛选和降解特性[J].应用与环境生物学报,2013,19(04):683-687.[doi:10.3724/SP.J.1145.2013.00683]
　LU Chenxia,LIU Long,LI Jianghua,et al.Isolation and Characterization of a Microorganism Degrading Starch/polyethylene Blends[J].Chinese Journal of Applied & Environmental Biology,2013,19(04):683-687.[doi:10.3724/SP.J.1145.2013.00683]
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淀粉填充聚乙烯类塑料降解微生物的筛选和降解特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年04期
页码:
683-687
栏目:
研究论文
出版日期:
2013-08-25

文章信息/Info

Title:
Isolation and Characterization of a Microorganism Degrading Starch/polyethylene Blends
作者:
陆辰霞; 刘龙; 李江华; 堵国成; 陈坚
（1江南大学工业生物技术教育部重点实验室 无锡 214122）
（2江南大学生物工程学院 无锡 214122）
（3江南大学食品科学与技术国家重点实验室 无锡 214122）
Author(s):
LU Chenxia;  LIU Long;  LI Jianghua;  DU Guocheng;  CHEN Jian
(1Key laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China)
(2School of Biotechnology, Jiangnan University, Wuxi 214122, China)
(3State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
关键词:
淀粉填充类塑料; 菌种筛选; 生物降解; 解淀粉芽孢杆菌
Keywords:
starch/polyethylene blends;  strain isolation;  biodegradation;  Bacillus amyloliquefaciens
分类号:
X172
DOI:
10.3724/SP.J.1145.2013.00683
文献标志码:
A
摘要:
为了加速塑料降解、减少环境污染，通过富集培养、透明圈、菌浓检测等方法从垃圾堆埋场土壤中筛选出降解淀粉填充聚乙烯（PE）类塑料的微生物1株，经16S rDNA同源性比对，鉴定出该菌株与解淀粉芽孢杆菌（Bacillus amyloliquefaciens）同源性为99%. 在基本培养基中利用该菌降解淀粉填充PE类塑料，通过失重、热重分析、扫描电镜法研究其降解特性，发现降解两周后塑料失重率达23.2%，淀粉含量减少13.8%，聚乙烯含量减少9.4%，并且塑料颗粒表面缺损变化明显. 研究表明该菌株能够高效降解淀粉填充PE类塑料，可用于该类塑料的回收利用和降解性能快速评价. 图6 表1 参26
Abstract:
In order to speed up plastic degradation and reduce contamination, a microorganism degrading starch/polyethylene blends was isolated from the soil of a wastes disposal site by the means of enrichment, clear zone and optical density (OD) detection. From the genetic homology analysis based on 16S rDNA sequence, the isolated strain was identified to be closely related to Bacillus amyloliquefaciens. To clarify the microbial degradability for starch/polyethylene blends, the degradation was investigated in minimal salt medium (MSM) with starch/polyethylene blend particles as sole carbon source, with its biodegradability verified by weight loss, thermogravimetric analysis and scanning electron microscope (SEM) analysis. After 14 days of biodegradation, 23.2% of the particles, 13.8% of starch and 9.4% of PE in particles were degraded, accompanied with obvious surface morphologic changes. The results show that the isolated strain can degrade starch/polyethylene blends efficiently and be applied in plastic recycle and rapid evaluation of biodegradability. Fig 6, Tab 1, Ref 26

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备注/Memo

备注/Memo:
国家质检总局质检公益性行业科研专项（200910036）资助 Supported by the General Administration of Quality Supervision for the Public Welfare Industry Research Special Project of China (No. 200910036)
更新日期/Last Update: 2013-08-22